The Neighbor net analysis identifies seven distinct genetic clusters.

<p>Neighbor-Net analysis of the concatenated nucleotide sequences based on uncorrected p-distance matrices. Bootstrap values (1,000 replicates) are shown on the edges (percentages). Seven different genetic clusters were identified (I to VII). The MLEE-based taxa and MLSA-based clusters (I to VII) are color-coded. Sub-networks of clusters I, II, IV, VI and VII are magnified and color-coded. Clusters III and V are not magnified due to the low number of genotypes.</p

Geographical distribution of cluster II, VI and VII genotypes.

<p>The neighbor-Net analyses of clusters II, VI and VII (defined in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002255#pntd-0002255-g001" target="_blank">Figure 1</a>) are represented. The genotypes are color-coded according to their geographical origins (detailed in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002255#pntd.0002255.s009" target="_blank">Table S1</a>).</p

Individual Neighbor net analysis of the seven housekeeping genes.

<p>Neighbor-Net analysis of each of the seven gene sequences based on uncorrected p-distance matrices. The seven genetic clusters are color-coded according to <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002255#pntd-0002255-g001" target="_blank">Figure 1</a>.</p

Disseminated infection rate, transmission rate and transmission efficiency to CHIKV strains of <i>Ae. albopictus</i> from France (AL-CSM) and <i>Ae. aegypti</i> from India (AA-INDIA) at different days post-infection.

<p>DIR: Disseminated infection rate corresponding to the proportion of mosquitoes with infected head among tested ones; TR: Transmission rate corresponding to the proportion of mosquitoes with infectious saliva among mosquitoes able to disseminate the virus beyond the midgut barrier; TE: Transmission efficiency corresponding to the proportion of mosquitoes with infectious saliva among tested ones; In parenthesis for DIR, number of mosquitoes analyzed; In parenthesis for TR, number of mosquitoes able to disseminate the virus beyond the midgut; In parenthesis for TE, number of mosquitoes analyzed; ^† Strain isolated from a French autochthonous case; ^‡ Strain isolated from a French imported case.</p

Transmission of CHIKV in saliva of <i>Ae. albopictus</i> from France (AL-CSM) and <i>Ae. aegypti</i> from India (AA-India).

<p>At days 3, 6, 9, and 14 after infection with an infectious blood-meal, mosquitoes were sacrificed and saliva was collected from individual mosquitoes and titrated by focus fluorescent assay on C6/36 <i>Ae. albopictus</i> cells. Two viral strains were tested: (A) CHIKV-1630 strain that circulated in India in 2010 and (B) CHIKV-1909 strain isolated from a CHIKV autochthonous case from Fréjus. Error bars refer to the standard error between different mosquitoes.</p

Dissemination of CHIKV in <i>Ae. albopictus</i> from France (AL-CSM) and <i>Ae. aegypti</i> from India (AA-India).

<p>At days 3, 6, 9, and 14 after infection with an infectious blood-meal, mosquitoes were sacrificed and heads were removed for viral titration. The number of infectious particles per head homogenate was estimated by focus fluorescent assay on C6/36 <i>Ae. albopictus</i> cells. Two viral strains were tested: (A) CHIKV-1630 strain that circulated in India in 2010 and (B) CHIKV-1909 strain isolated from a CHIKV autochthonous case from Fréjus. Error bars refer to the standard error between different mosquitoes.</p

Disseminated infection rate, transmission rate and transmission efficiency to DENV strains of <i>Ae. albopictus</i> from France (AL-CSM) and <i>Ae. aegypti</i> from Martinique (AA-Martinique) at two days post-infection.

<p>DIR: Disseminated infection rate corresponding to the proportion of mosquitoes with infected head among tested ones; TR: Transmission rate corresponding to the proportion of mosquitoes with infectious saliva among mosquitoes able to disseminate the virus beyond the midgut barrier; TE: Transmission efficiency corresponding to the proportion of mosquitoes with infectious saliva among tested ones; In parenthesis for DIR, number of mosquitoes analyzed; In parenthesis for TR, number of mosquitoes able to disseminate the virus beyond the midgut; In parenthesis for TE, number of mosquitoes analyzed;</p>†<p>Strain isolated from a French autochthonous case;</p>‡<p>Strain isolated from a French imported case.</p

Transmission of DENV in saliva of <i>Ae. albopictus</i> from France (AL-CSM) and <i>Ae. aegypti</i> from Martinique (AA-Martinique).

<p>At days 9 and 14 after infection with an infectious blood-meal provided, mosquitoes were sacrificed and saliva was collected from individual mosquitoes and titrated by focus fluorescent assay on C6/36 <i>Ae. albopictus</i> cells. Two viral strains were tested: (A) DENV-2025 strain that circulated in Martinique in 2010 and (B) DENV-1806 strain isolated from a DENV autochthonous case from Nice. Error bars refer to the standard error between different mosquitoes.</p

RepPCR (Diversilab®) and UPGMA dendrogram obtained with 41 A/B1 CVP+ strains and 9 ECOR strains.

<p>(1) Phylogenetic group, as determined by PCR; (2) Phylogenetic group of ECOR strains; (3) ST: sequence type according to Achtmann's scheme; (4) STc: sequence type complexes according to Achtmann's scheme (corresponding phylogenetic group in brackets); (5) PCR O78. Strains were responsible for Urinary Tract nfection (UTI), Neonatal Meningitis (NM) or Bacteremia (B). Clusters of clinical isolates are framed in black and ECOR strains are underlined.</p

Epidemiological, phenotypic and genotypic features of the <i>A. baumannii</i> studied.

<p>PFGE, pulsed-field gel electrophoresis; MLST, Multilocus sequence typing; ST, sequence type;</p><p>IMP, imipenem; MEM, meropenem.</p>a<p>Number of strains belonging to a single ST.</p>b<p>These PFGE types were each observed in two distinct centres.</p><p>Epidemiological, phenotypic and genotypic features of the <i>A. baumannii</i> studied.</p