ROC curves were constructed to compare ELISPOT results when the cells were stimulated with rMtb-HBHA (A), PPD (B), and peptide mixtures of ESAT-6 and CFP-10 (C–D).

<p>The curves were established for infected (Active and Inactive TB) and the healthy control group. The calculated AUC and the respective confidence intervals (in brackets) are shown for each tested antigen.</p

Characteristics of the individuals enrolled in the study.

<p>Characteristics of the individuals enrolled in the study.</p

Production of IFNγ by lymphocytes stimulated with ESAT-6 (Panel A) and CFP-10 (Panel B) was tested in the ELISPOT technique.

<p>The groups of patients with inactive TB (n = 5), active TB (n = 6) and healthy vaccinated subjects (n = 13) were tested. Cell-mediated immunological responses were determined as the number of reactive cells per 10⁶ lymphocytes. The lower dotted line is the level of positivity suggested by the manufacturer, the upper dotted line is the level of positivity adopted at the HUSLAB diagnostic laboratory. The area between the two dotted lines represents the so-called grey-zone, an area of uncertainty for interpretation that was calculated based on assay imprecision (data not published).</p

The ability of rMtb-HBHA and PPD to induce the production of IFNγ was tested in the ELISPOT technique.

<p>The groups of patients with inactive TB (n = 5); active TB (n = 6), healthy young (n = 7) and middle-age (n = 8) vaccinated subjects and patients with isolation of so-called atypical mycobacteria (n = 4) and were enrolled. Comparison of the cell-mediated immunological responses to rMtb-HBHA was performed as the determination of the number of reactive cells per10⁶ lymphocytes (A) and as the measurement of IFNγ production activities expressed as arbitrary units (B). Cell-mediated immunological responses in healthy vaccinated individuals was studied by division by age into two groups (C). The correlation of rMtb-HBHA with the PPD ELISPOT reactivities expressed as the frequencies of reactive cells per10⁶ is presented in (D). Data are shown as individual reactivities; the horizontal bars represent arithmetic median values.</p