2 research outputs found
Protective Effects of Bovine Serum Albumin on Superparamagnetic Iron Oxide Nanoparticles Evaluated in the Nematode Caenorhabditis elegans
Nanomaterials give rise to unique
biological reactivity that needs
to be thoroughly investigated. The quest for enhanced magnetic nanomaterials
of different shapes, magnetic properties, or surface coatings continues
for applications in drug delivery, targeting therapies, biosensing,
and magnetic separation. In this context, the use of simple in vivo
models, such as Caenorhabditis elegans, to biologically evaluate nanoparticles is currently in increasing
demand as it offers low-cost and information-rich experiments. In
this work, we evaluated how surface modification (citrate- and protein-coated)
of superparamagnetic iron oxide nanoparticles (C-SPIONs and BSA-SPIONs,
respectively) induces changes in their toxicological profile and biodistribution
using the animal model C. elegans and
combining techniques from materials science and biochemistry. The
acute toxicity and nanoparticle distribution were assessed in two
populations of worms (adults and larvae) treated with both types of
SPIONs. After 24 h treatment, nanoparticles were localized in the
alimentary system of C. elegans; acute
toxicity was stronger in adults and larvae exposed to C-SPIONs rather
than BSA-SPIONs. Adult uptake was similar for both SPION types, whereas
uptake in larvae was dependent on the surface coating, being higher
for BSA-SPIONs. Nanoparticle size was evaluated upon excretion, and
a slight size decrease was found. Interestingly, all results indicate
the protective effects of the BSA to prevent degradation of the nanoparticles
and decrease acute toxicity to the worms, especially at high concentrations.
We argue that this relevant information on the chemistry and toxicity
of SPIONs in vivo could not be gathered using more classical in vitro
approaches such as cell culture assays, thus endorsing the potential
of C. elegans to assess nanomaterials
at early stages of their synthetic formulations
Protective Effects of Bovine Serum Albumin on Superparamagnetic Iron Oxide Nanoparticles Evaluated in the Nematode Caenorhabditis elegans
Nanomaterials give rise to unique
biological reactivity that needs
to be thoroughly investigated. The quest for enhanced magnetic nanomaterials
of different shapes, magnetic properties, or surface coatings continues
for applications in drug delivery, targeting therapies, biosensing,
and magnetic separation. In this context, the use of simple in vivo
models, such as Caenorhabditis elegans, to biologically evaluate nanoparticles is currently in increasing
demand as it offers low-cost and information-rich experiments. In
this work, we evaluated how surface modification (citrate- and protein-coated)
of superparamagnetic iron oxide nanoparticles (C-SPIONs and BSA-SPIONs,
respectively) induces changes in their toxicological profile and biodistribution
using the animal model C. elegans and
combining techniques from materials science and biochemistry. The
acute toxicity and nanoparticle distribution were assessed in two
populations of worms (adults and larvae) treated with both types of
SPIONs. After 24 h treatment, nanoparticles were localized in the
alimentary system of C. elegans; acute
toxicity was stronger in adults and larvae exposed to C-SPIONs rather
than BSA-SPIONs. Adult uptake was similar for both SPION types, whereas
uptake in larvae was dependent on the surface coating, being higher
for BSA-SPIONs. Nanoparticle size was evaluated upon excretion, and
a slight size decrease was found. Interestingly, all results indicate
the protective effects of the BSA to prevent degradation of the nanoparticles
and decrease acute toxicity to the worms, especially at high concentrations.
We argue that this relevant information on the chemistry and toxicity
of SPIONs in vivo could not be gathered using more classical in vitro
approaches such as cell culture assays, thus endorsing the potential
of C. elegans to assess nanomaterials
at early stages of their synthetic formulations