Relationship between polyGT allele length and nausea sub-score.

<p>In patients with diabetic gastroparesis, the relationship between polyGT allele length and nausea sub-score on the GCSI questionnaire fit to a slope that was significantly different from zero (P < 0.006 by linear regression, dotted lines are 95% confidence intervals) and b) subjects with one or two long alleles (open circles in Fig 4a) had significantly higher nausea sub-scores than subjects with zero long alleles (closed circles in Fig 4a, P = 0.022, Mann Whitney test). Whiskers are the medians with the interquartile ranges for the nausea sub-score.</p

Size distribution of the polyGT repeat lengths.

<p>a) The size distribution of the polyGT repeat length in type 2 diabetic controls is significantly longer than the allele length in non-diabetic controls. The fractional frequency distribution of the lengths of polyGT repeat in <i>HMOX1</i> from non-diabetic control subjects with no GI motility disorders (n = 170) compared to subjects with type 2 diabetes and no GI motility disorders (n = 84) are shown (P < 0.05 Dunn’s test). b) Fractional frequency distribution of the lengths of polyGT repeat in <i>HMOX1</i> from subjects with type 2 diabetes and no GI motility disorders (n = 84) compared to subjects with type 2 diabetes and gastroparesis (n = 72, P = NS, Dunn’s test). c) Fractional frequency distribution of the lengths of polyGT repeat in <i>HMOX1</i> from subjects with type 1 diabetes and no GI motility disorders (n = 84) compared to subjects with type 1 diabetes and gastroparesis (n = 99, P = NS, Dunn’s test). Allele length was determined by PCR amplification of genomic DNA from blood using the ABI 3730 platform and capillary electrophoresis and using primers flanking the GT repeat region.</p

Data on subjects that were studied.

<p>Data on subjects that were studied.</p

Allele distribution in gastroparetic and control subjects.

<p>Allele distribution of long (L), medium (M) and short (S) alleles a) for subjects with gastroparesis is significantly different from the distribution for control subjects (Chi² P value = 0.019) and b) for subjects with idiopathic gastroparesis compared to non-diabetic controls (Chi² P value = 0.049). Short alleles were defined as shorter than 29 polyGT repeats and long alleles were defined as longer than 32 repeats (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187772#pone.0187772.g001" target="_blank">Fig 1a</a> for definition).</p

Frequency distribution of the lengths of the polyGT repeat allele in the <i>HMOX1</i> gene.

<p>a) Frequency distribution of the lengths of the polyGT repeat allele in the <i>HMOX1</i> gene from control subjects with no diabetes and no symptoms of gastroparesis. Dotted lines indicate division chosen between short, medium and long alleles. b) Fractional frequency distribution of the lengths of polyGT repeat in <i>HMOX1</i> from control, non-diabetic subjects (n = 170) compared to all gastroparetic subjects (n = 576). The distribution of the allele lengths was significantly different between the groups (P = 0.004, Mann Whitney test). c) Distribution of allele lengths in subjects with type 2 diabetes and gastroparesis, d) type 1 diabetes and gastroparesis, e) idiopathic gastroparesis. Allele length was determined by PCR amplification of genomic DNA from blood using the ABI 3730 platform and capillary electrophoresis and using primers flanking the GT repeat region.</p

Data on gastroparetic subjects that were studied.

<p>Data on gastroparetic subjects that were studied.</p