Potentialization of N-a-tosyl-L-phenylalanine chloromethyl ketone (TPCK) cytotoxic activity by 2-(1-adamantylamino)-6-methylpyridine (AdAMP) in human ovarian cancer cells

Objectives: TNF is one of the key cytokines involved in cancer development. TNF signaling can result in both stimulating and inhibitory signals that can result in opposite biological effects in cancerogenesis. 2-(1-adamantylamino)-6-methylpyridine (AdAMP) enhances TNF secretion whereas N-a-tosyl-L-phenylalanine chloromethyl ketone (TPCK) is a NF-κB inhibitor potentially stimulating proapoptotic TNF signals. The aim of the study was to assess the effect of TPCK in combination with AdAMP on human ovarian cells. Material and methods: CAOV-1 human ovarian cell line was incubated with TPCK and AdAMP for 24 hours. The cytotoxic effect was evaluated in a crystal violet assay. A monoclonal antibody against TNF, Infliximab, was added to examine the possible mechanism of interactions. Results: Depending on concentration, AdAMP potentialized cytotoxic activity of TPCK or had a synergistic effect with TPCK. Infliximab did not reverse cytotoxicity of AdAMP and TPCK and in some cytotoxic and non-cytotoxic concentrations even enhanced their cytotoxicity. Conclusions: AdAMP and TPCK cytotoxicity seems to be dependent on TNF signaling, however, the exact mechanism of interactions remains unclear

Antitumor effects of sulindac in ovarian cell cultures

Abstract Objective: The purpose of our study was to assess susceptibility of cells of various ovarian cell lines on different nonsteroidal anti-inflammatory drugs (NSAIDs). Materials and methods: Cytotoxic effect of NSAIDs was tested using MTT colorimetric assay. Results: Amongst 6 NSAIDs tested: sulindac, sulindac sulfide, sulindac sulfone, acetylsalicylic acid, nimesulide, and rofecoxib, viability of ovarian carcinoma cells was compromised most strongly by sulindac and sulindac sulfide and concerned all the cell lines tested: SKOV-3, MDAH 2774, OVCA-1, and OVP-10. Sulindac sulfone and rofecoxib also displayed some cytotoxic effect during prolonged 72-hour incubation. Other NSAIDs tested: nimesulide and acetylsalicylic acid were devoid of cytotoxic effect on ovarian cancer cells. Conclusion: Our results are encourage enough to conduct clinical trials that could allow to draw conclusions regarding potential application of sulindac in the adjuvant treatment of a standard chemotherapy of ovarian cancer

Immunomodulatory effects of inosine pranobex on cytokine production by human lymphocytes

Inosine pranobex (inosine dimepranol acedoben, isoprinosine) (Inos) is an immunomodulatory and antiviral drug used in some viral infections, especially in patients with weakened immunity. In the present study, effects of Inos on the production of cytokines attributable to Th1 (IL-2, IFN-γ, and TNF-α) or Th2 cells (IL-4, IL-5, and IL-10) were tested in human peripheral blood lymphocyte cultures stimulated with phytohemagglutinin (PHA). Inos enhanced TNF-α secretion significantly (in short-term – 24-hour, and prolonged term – 72-hour cultures) and IFN-γ (in 72-hour cultures). Surprisingly, production of IL-10 by PHA-stimulated lymphocytes was suppressed by Inos in a dose-dependent manner in both 24-hour and 72-hour cultures. These results shed some light on immunomodulatory properties of Inos and suggest applicability of this agent in patients with a depressed function of the immune system

Wewnątrznaczyniowa terapia niedokrwiennego udaru mózgu — doniesienie wstępne nt. doświadczeń jednego ośrodka

A thrombolytic therapy is still more and more commonly used treatment in the cases of the brain ischemia of the vascular origin. Despite the growing experience in this kind of therapy, its clinical efficacy seems to be still not sufficient. Looking for the new ways of the acute brain ischemia treatment, the potential role of the brain vessel thrombectomy is taken into account. In the paper, the own experiences concerning the treatment of 28 patients with ischemic stroke who underwent intracranial endovascular interventions due to the acute vessel occlusion, are presented.Leczenie trombolityczne udaru mózgu jest coraz częściej stosowanym postępowaniem terapeutycznym w przypadku niedokrwienia mózgu pochodzenia naczyniowego. Ciągle jednak jego skuteczność, jak i możliwości zastosowania wydają się niewystarczające. Stąd też zrozumiałe wydaje się coraz większe zainteresowanie w ostatnich latach metodami mechanicznej trombektomii naczyń wewnątrzczaszkowych w leczeniu przyczynowym niedokrwiennego udaru mózgu. W pracy przedstawiono własne doświadczenia w leczeniu wewnątrznaczyniowym w grupie 29 chorych z udarem niedokrwiennym mózgu, u których wykonano interwencje wewnątrznaczyniowe w obrębie naczyń wewnątrzczaszkowym z powodu ich ostrej niedrożności

Recanalisation of cerebral artery aneurysms treated endovascularly — a midterm follow-up

Endovascular methods of aneurysm treatment, as an alternative to neurosurgical clipping, have proved a welcome opportunity to treat patients with unruptured aneurysms or those disqualified from neurosurgical intervention. This paper presents our own experience of endovascular treatment of cerebral aneurysms in 107 patients. It includes clinical and technical data from the perioperative period and a 12-month radiological follow-up of 78 patients. Method. Our retrospective evaluation covered patients with intracranial aneurysms treated endovascularly. The following were analysed: age, sex, neurological symptoms, and familial burden of intracranial aneurysm. Multivariate analysis was performed to determine independent factors of recanalisation of the cerebral aneurysm 12 months after embolisation. Results. The data of 107 patients at a mean age of 61 years [57.09 ± 14.27] treated with embolisation was analysed. The indication for intervention in 16 patients was subarachnoid haemorrhage; in the remaining 91 cases, aneurysms were revealed during diagnostic procedures for different symptoms or during imaging examinations. The intracranial segment of the internal carotid artery and the anterior communicating artery were the most common locations for aneurysms. After embolisation, subarachnoid haemorrhage occurred in one patient, ischaemic stroke in two patients, and one patient died because of acute circulatory insufficiency. The functional status of 94 patients on the day of discharge from the department (on days 4-21) was very good. 78 patients completed a 12-month follow-up period. In 11 of those, a follow-up MR angiography revealed recanalisation 12 months after the intervention. Except for one patient reporting vertigo, aneurysm recanalisation procedures were asymptomatic. The only independent risk factor for recanalisation was the size of aneurysm > 10 mm; OR 3.0; CI [1.15–7.83] p = 0.0255. Conclusions. Embolisation of cerebral aneurysms is a safe method with few perioperative complications, and most of these are mild and transient.The size of the aneurysm during qualification for embolisation is a risk factor for recanalisation in the subsequent 12 months. Recanalisation of embolised cerebral aneurysms concerns less than 20% of patients in a one-year follow-up and is most often asymptomatic

Statins Impair Antitumor Effects of Rituximab by Inducing Conformational Changes of CD20

Jakub Golab and colleagues found that statins significantly decrease rituximab-mediated complement-dependent cytotoxicity and antibody-dependent cellular cytotoxicity against B cell lymphoma cells

Mechanical thrombectomy in acute stroke – Five years of experience in Poland

Objectives Mechanical thrombectomy (MT) is not reimbursed by the Polish public health system. We present a description of 5 years of experience with MT in acute stroke in Comprehensive Stroke Centers (CSCs) in Poland. Methods and results We retrospectively analyzed the results of a structured questionnaire from 23 out of 25 identified CSCs and 22 data sets that include 61 clinical, radiological and outcome measures. Results Most of the CSCs (74%) were founded at University Hospitals and most (65.2%) work round the clock. In 78.3% of them, the working teams are composed of neurologists and neuro-radiologists. All CSCs perform CT and angio-CT before MT. In total 586 patients were subjected to MT and data from 531 of them were analyzed. Mean time laps from stroke onset to groin puncture was 250±99min. 90.3% of the studied patients had MT within 6h from stroke onset; 59.3% of them were treated with IV rt-PA prior to MT; 15.1% had IA rt-PA during MT and 4.7% – emergent stenting of a large vessel. M1 of MCA was occluded in 47.8% of cases. The Solitaire device was used in 53% of cases. Successful recanalization (TICI2b–TICI3) was achieved in 64.6% of cases and 53.4% of patients did not experience hemorrhagic transformation. Clinical improvement on discharge was noticed in 53.7% of cases, futile recanalization – in 30.7%, mRS of 0–2 – in 31.4% and mRS of 6 in 22% of cases. Conclusion Our results can help harmonize standards for MT in Poland according to international guidelines

Od immunologii do modelowania, przetwarzania i analiz danych

Observation of nature is often the inspiration for unconventional and innovative ideas, proposals, suggestions, overall concepts for carrying out modeling phenomena and technical processes, economic, processing and analysis of statistical data. Artificial neural networks, genetic algorithms and evolutionary algorithms were created as an imitation of biological solutions. In recent years, the rapid development of immunological algorithms which are based on the action of the human immune system, has occurred. These algorithms are used in mathematics, engineering, problem solving in decision making, management, economics, finance. At the beginning of this article we describe the action of the human immune system, which is the basis for the construction of immune algorithms. Then we present the most important mechanisms influencing the operation and construction of the algorithms based on biological mechanisms of the immune system, in particular the negative selection, immune network, clonal selection. Then we describe how the elements and mechanisms of artificial immune system algorithms are represented to enable modeling, processing and analysis of data. In the last part of the article to illustrate the wide range of possibilities of using immune algorithms to solve various sorts of problems from a completely different fields, we present examples of applications that involve the assessment of bank borrowers, the task of coloring a graph belonging to combinatorial optimization problems, a multi-dimensional classification, fault detection

Zwężenie światła i masywne zwapnienia jako następstwa zakrzepicy w żyle głównej dolnej : obrazowanie w spiralnej tomografii komputerowej z zastosowaniem techniki modelowania

Background: Thrombosis of the IVC is a rare and often under-recognized problem. It is mostly associated with deep-vein thrombosis, but there are several conditions which may lead to IVC thrombosis as well. Complications of IVC thrombosis may be of embolic or thrombotic nature. Post-thrombotic IVC calcifications have been rarely described. Case report: We report of a 42-year-old man with a history of DVT who complained of abdominalgia and developed venous collateral circulation on the anterior thoracic and abdominal wall. Using Doppler ultrasound and CTA, massive calcifications and a critical stricture of the infra-renal portion of the IVC were diagnosed. Conclusions: The authors describe the imaging procedures used to diagnose this case and give a short review of literature concerning similar cases