Total ionospheric electron content calibration using SERIES GPS satellite data

The current status of the Deep Space Network advanced systems research into ionospheric calibration techniques, based on Global Positioning System (GPS) data is described. A GPS-based calibration system is planned to replace the currently used Faraday rotation method by 1989. The SERIES receiver system determines the differential group delay of signals transmitted at two different carrier frequencies. This differential delay includes an ionospheric component and a GPS transmitter offset. The transmitter offsets are different for each GPS satellite. Tests were conducted to assess the effect of the offsets on the ionospheric calibration accuracy. From the obtained data, the total electron content and GPS transmitter offsets are calculated by a least squares estimation method employing a local model of total ionospheric electron content. The end product is an estimation of the total ionospheric content for an arbitrary line-of-sight direction. For the presented polynomial fitting technique, the systematic error due to mismodeling is estimated to be approximately 6 x 10 to the 16th power el/sq m, while the formal error is approximately 2 x 10 to the 16th power el/sq m. The final goal is an error of 3 x 10 to the 16th power el/sq m (approximately 0.7 ns at 2.3 GHz)

Archaebacterial rhodopsin sequences: Implications for evolution

It was proposed over 10 years ago that the archaebacteria represent a separate kingdom which diverged very early from the eubacteria and eukaryotes. It follows that investigations of archaebacterial characteristics might reveal features of early evolution. So far, two genes, one for bacteriorhodopsin and another for halorhodopsin, both from Halobacterium halobium, have been sequenced. We cloned and sequenced the gene coding for the polypeptide of another one of these rhodopsins, a halorhodopsin in Natronobacterium pharaonis. Peptide sequencing of cyanogen bromide fragments, and immuno-reactions of the protein and synthetic peptides derived from the C-terminal gene sequence, confirmed that the open reading frame was the structural gene for the pharaonis halorhodopsin polypeptide. The flanking DNA sequences of this gene, as well as those of other bacterial rhodopsins, were compared to previously proposed archaebacterial consensus sequences. In pairwise comparisons of the open reading frame with DNA sequences for bacterio-opsin and halo-opsin from Halobacterium halobium, silent divergences were calculated. These indicate very considerable evolutionary distance between each pair of genes, even in the dame organism. In spite of this, three protein sequences show extensive similarities, indicating strong selective pressures

The effect of the dynamic wet troposphere on VLBI measurements

Calculations using a statistical model of water vapor fluctuations yield the effect of the dynamic wet troposphere on Very Long Baseline Interferometry (VLBI) measurements. The statistical model arises from two primary assumptions: (1) the spatial structure of refractivity fluctuations can be closely approximated by elementary (Kolmogorov) turbulence theory, and (2) temporal fluctuations are caused by spatial patterns which are moved over a site by the wind. The consequences of these assumptions are outlined for the VLBI delay and delay rate observables. For example, wet troposphere induced rms delays for Deep Space Network (DSN) VLBI at 20-deg elevation are about 3 cm of delay per observation, which is smaller, on the average, than other known error sources in the current DSN VLBI data set. At 20-deg elevation for 200-s time intervals, water vapor induces approximately 1.5 x 10 to the minus 13th power s/s in the Allan standard deviation of interferometric delay, which is a measure of the delay rate observable error. In contrast to the delay error, the delay rate measurement error is dominated by water vapor fluctuations. Water vapor induced VLBI parameter errors and correlations are calculated. For the DSN, baseline length parameter errors due to water vapor fluctuations are in the range of 3 to 5 cm. The above physical assumptions also lead to a method for including the water vapor fluctuations in the parameter estimation procedure, which is used to extract baseline and source information from the VLBI observables

School racial climate and discipline practices: A systematic review

The disproportionate use of exclusionary discipline with African American students in American schools is systemic and well documented in the literature. School climate has been found to be related to suspension rates and as the literature has begun to demonstrate the differences in how African American students experience their school’s climate when compared to White students, an area of intervention has revealed itself. As school psychologists begin work improving school climates with the intentional goal of reducing the racial discipline gap, they may find success by improving school climate, and more specifically, the school’s racial climate. The current systematic review explored what relationship, if any, exists between school racial climate and discipline within K-12 schools. After a search of databases, six studies were selected. While none measured school racial climate explicitly as described in Byrd (2017)’s framework, the selected studies were included because they directly measure discipline in K-12 schools and school climate by racial group or as it affects racial discipline disparities. Results indicated that schools with an authoritative climate have fewer suspensions overall but not necessarily a smaller racial discipline gap; and several other aspects of school climate including positive student-teacher relationships, disciplinary structure, and African American students’ perceptions of equity and sense of belonging are associated with smaller racial discipline gaps. Implications for future research and school psychologists’ practice are discussed

Municipal Torts: The Rule Is Liability—The Exception Is Immunity

Enghauser Manufacturing Co. v. Eriksson Engineering Ltd., 6 Ohio St. 3d 31, 451 N.E.2d 228 (1983)

Halide binding by the purified halorhodopsin chromoprotein. II. New chloride-binding sites revealed by 35Cl NMR

Halorhodopsin is a light-driven chloride pump in the cell membrane of Halobacterium halobium. Recently, a polypeptide of apparent Mr = 20,000 has been purified that contains the halorhodopsin chromophore. Here we use 35Cl NMR to show that the purified chromoprotein possesses two previously unknown classes of chloride-binding sites. One class exhibits a low affinity (KD much greater than 1 M) for chloride and bromide. The second class exhibits a higher affinity (KD = 110 ± 50 mM) for chloride and also binds other anions according to the affinity series I-, SCN- greater than Br-, NO-3 greater than Cl- greater than F- , citrate. Both classes of NMR site remain intact at pH 11, indicating that the essential positive charges are provided by arginine. Also, both classes are unaffected by bleaching, suggesting that the sites are not in the immediate vicinity of the halorhodopsin chromophore. Although the chromoprotein also appears to contain the chloride- transport site (Steiner, M., Oesterhelt, D., Ariki, M., and Lanyi, J. K. (1984) J. Biol. Chem. 259, 2179-2184), this site was not detected by 35Cl NMR, suggesting that the transport site is in the interior of the protein where it is sampled slowly by chloride in the medium. It is proposed that the purified chromoprotein possesses a channel leading from the medium to the transport site and that the channel contains the high affinity NMR site which facilitates the migration of chloride between the medium and the transport site. We have also used 35Cl NMR to study chloride binding to purified monomeric bacteriorhodopsin; however, this protein contains no detectable chloride-binding sites

Protein structural change at the cytoplasmic surface as the cause of cooperativity in the bacteriorhodopsin photocycle

The effects of excitation light intensity on the kinetics of the bacteriorhodopsin photocycle were investigated. The earlier reported intensity-dependent changes at 410 and 570 nm are explained by parallel increases in two of the rate constants, for proton transfers to D96 from the Schiff base and from the cytoplasmic surface, without changes in the others, as the photoexcited fraction is increased. Thus, it appears that the pKa of D96 is raised by a cooperative effect within the purple membrane. This interpretation of the wild-type kinetics was confirmed by results with several mutant proteins, where the rates are well separated in time and a model-dependent analysis is unnecessary. Based on earlier results that demonstrated a structural change of the protein after deprotonation of the Schiff base that increases the area of the cytoplasmic surface, and the effects of high hydrostatic pressure and lowered water activity on the photocycle steps in question, we suggest that the pKa of D96 is raised by a lateral pressure that develops when other bacteriorhodopsin molecules are photoexcited within the two-dimensional lattice of the purple membrane. Expulsion of no more than a few water molecules bound near D96 by this pressure would account for the calculated increase of 0.6 units in the pKa