DataSheet_1_Integrated profiling of endoplasmic reticulum stress-related DERL3 in the prognostic and immune features of lung adenocarcinoma.pdf

BackgroundDERL3 has been implicated as an essential element in the degradation of misfolded lumenal glycoproteins induced by endoplasmic reticulum (ER) stress. However, the correlation of DERL3 expression with the malignant phenotype of lung adenocarcinoma (LUAD) cells is unclear and remains to be elucidated. Herein, we investigated the interaction between the DERL3 and LUAD pathological process.MethodsThe Cancer Genome Atlas (TCGA) database was utilized to determine the genetic alteration of DERL3 in stage I LUAD. Clinical LUAD samples including carcinoma and adjacent tissues were obtained and were further extracted to detect DERL3 mRNA expression via RT-qPCR. Immunohistochemistry was performed to evaluate the protein expression of DERL3 in LUAD tissues. The GEPIA and TIMER website were used to evaluate the correlation between DERL3 and immune cell infiltration. We further used the t-SNE map to visualize the distribution of DERL3 in various clusters at the single-cell level via TISCH database. The potential mechanisms of the biological process mediated by DERL3 in LUAD were conducted via KEGG and GSEA.ResultsIt was indicated that DERL3 was predominantly elevated in carcinoma compared with adjacent tissues in multiple kinds of tumors from the TCGA database, especially in LUAD. Immunohistochemistry validated that DERL3 was also upregulated in LUAD tissues compared with adjacent tissues from individuals. DERL3 was preliminarily found to be associated with immune infiltration via the TIMER database. Further, the t-SNE map revealed that DERL3 was predominantly enriched in plasma cells of the B cell population. It was demonstrated that DERL3 high-expressed patients presented significantly worse response to chemotherapy and immunotherapy. GSEA and KEGG results indicated that DERL3 was positively correlated with B cell activation and unfolded protein response (UPR).ConclusionOur findings indicated that DERL3 might play an essential role in the endoplasmic reticulum-associated degradation (ERAD) process in LUAD. Moreover, DERL3 may act as a promising immune biomarker, which could predict the efficacy of immunotherapy in LUAD.</p

Measurement and Correlation of Solubility of Cefathiamidine in Water + (Acetone, Ethanol, or 2‑Propanol) from (278.15 to 308.15) K

In this study, the solubility of cefathiamidine was measured in water + (acetone, ethanol, or 2-propanol) at temperature from (278.15 to 313.15) K by using a gravimeteic method. The solubility of cefathiamidine increased with increasing temperature. Besides, at a given temperature, solubility in different mixtures follows different rules. In water + (acetone or 2-propanol), it decreases with the increase of the initial molar fraction of acetone or 2-propanol; whereas synergistic effect of mixed solvents was observed in water + ethanol mixtures, and solubility reached maximum at the ethanol molar fraction of 0.4. The experimental data were well correlated by the modified Apelblat equation, the CNIBS/R-K model, the combined version of the Jouyban–Acree and van’t Hoff model, and the combined version of the Jouyban–Acree and the modified Apelblat model. Furthermore, the thermodynamic functions of dissolution of cefathiamidine in different mixtures were obtained based on the van’t Hoff equation and the modified Apelblat equation. The results indicate that the dissolution process of cefathiamidine is endothermic

Solubility Correlation and Thermodynamic Analysis of Sorafenib Free Base and Sorafenib Tosylate in Monosolvents and Binary Solvent Mixtures

The solubility of sorafenib free base (SFB) and sorafenib tosylate (ST) in five monosolvents and binary solvents of 2-propanol + 1,4-dioxane was measured over the temperature ranged from 283.15 to 333.15 K by using a UV spectroscopy method. The solubility of SFB and ST in different monosolvents increases with increasing temperature, while in the binary solvents, the solubility shows the maximum value at 0.50 and 0.75 2-propanol mole fraction for SFB and ST, respectively. The Apelblat model and the CNIBS/R-K model were applied to correlate the solubility data, which shows that the two selected thermodynamic models could give satisfactory results. Moreover, mixing thermodynamic properties of enthalpy, entropy, and Gibbs free energy of SFB and ST were obtained based on the nonrandom two-liquid model for further understanding of the mixing behavior