725 research outputs found
Early development of the human mesonephros
The mesonephrogenic cord disintegrates into approximately 35-40 provesicular cell masses which are in close contact with the mesonephric (Wolffian) duct (WD) on their lateral side. Here, the epithelium of the WD is columnar and shares a common basal lamina with the provesicular cell masses. This in turn gives rise to a sickle-shaped pseudostratified epithelium. The concavity of the sickle is filled by spherical cells, the transition of which into the surrounding connective tissue is continuous. The sickle is transformed into a distillation flask and becomes separated from the mesonephric duct while the spherical cells maintain a connection to it by a—for the time being—solid outlet pipe. The columnar epithelium of the mesonephric duct becomes a multilayered cone, whose surface is in contact with the outlet tube. Shortly after, a continuous lumen is formed in the cone and the outlet pipe which is delimited by cells becoming columnar and forming a basal lamina. The epithelial anlage of the nephron is clearly separated from the surrounding mesenchyma by these processes. The flask eventually becomes a corpusculum, the outlet pipe a secretory (proximal) as well as collecting tubule, and the cone of the mesonephric duct a mesoureter. The various sections display differentially differentiated epithelia that are clearly distinct from each other. The mesoureter behaves differently during differentiation of epi- and paragenitale: in the epigenitale, it is short and runs into the collecting tubules of the nephrons at the lateral side of the convolved tubules, whereas a long mesoureter crosses the dorsal side of the convolved tubules and joins the corresponding collecting tubules at the far end of the mesonephros in the paragenital
The value of different resistance parameters in distinguishing biopsy-proved dysfunction of renal allografts
The data concerning the value of duplex sonography in diagnosing parenchymatous renal allograft dysfunction are controversial. Most early studies did not take into consideration the many factors influencing resistance parameters. We therefore performed a prospective, biopsy-controlled study with exclusion of all known sources of error regarding resistance parameters. Furthermore we investigated the value of a new resistance parameter, the systolic deceleration percentage. Forty-seven duplex sonographic studies were performed on 43 patients (30 male, 13 female, median age 47 years, range 7-70). Fourteen studies were done on normally functioning grafts (control group) an average of 33 days after transplantation. Thirty-three studies were performed on dysfunctional grafts immediately prior to biopsy. Grafts which had been transplanted more than a year previously or with vascular findings or any other clinical or sonographic pathology probably explaining function deterioration were excluded. In all patients, the resistive index (RI), pulsatility index (PI) and systolic deceleration percentage (DP) were calculated in the main renal artery and in the interlobar artery. Of the 33 grafts with dysfunction, nine had vascular rejection (VR), 11 interstitial rejection (IR), 11 cyclosporin A toxicity (CAT) and two other histologies (OR). The mean RI in normal grafts (NO) was 0.71±0.06 in the main artery and 0.68±0.06 in the interlobar artery, in VR 0.86±0.12 and 0.80±0.18, in IR 0.72±0.05 and 0.70±0.07, in CAT 0.67±0.06 and 0.65±0.07 and in OR 0.64±0.07 and 0.60±0.01. For PI, the values were 1.45±0.23 and 1.41±0.28 (NO), 3.5±2.13 and 2.92±2.16 (VR), 1.55±0.26 and 1.46±0.33 (IR), 1.32±0.25 and 1.27±0.26 (CAT) and 1.30±0.34 and 1.13±0.04 (OR). For DP we calculated 28±5% and 29±6% (NO), 43±14% and 36±6% (VR), 29±9% and 27±9% (IR), 31±8% and 32±7% (CAT ) and 32±4% and 28±3% (OR). The sensitivity/specificity for VR with a cutoff mean+2 SD was 0.44/1 for RI, 0.55/0.97 for PI and 0.33/0.89 for DP. It was concluded that:(1) despite the high selection of our patient group, diagnostic accuracy of duplex sonography for diagnosing parenchymatous function disorder in renal allograft remains insufficient; (2) in vascular rejection only, the resistance parameters differ significantly from the values of normal allografts; (3) the higher the cutoff of resistance parameters, the better the specificity and the worse the sensitivity for diagnosing vascular rejection; (4) of all investigated resistance parameters, the RI is the most practical due to a simple measurement techniqu
Early renal transplant dysfunction due to arterial kinking stenosis
The main differential diagnoses of early renal trans-plant dysfunction include ischaemic damage, cyclospo-rin toxicity, and rejection [1]. Rarer causes include bleeding, ureteral obstruction, urinary leak, venou
Assessing Long-Term Trends In Vegetation Productivity Change Over the Bani River Basin in Mali (West Africa)
Using time series of Normalized Difference Vegetation Index (NDVI) and rainfall
data, we investigated historical vegetation productivity trends from 1982 to 2011
over the Bani River Basin in Mali. Statistical agreements between long-term trends
in vegetation productivty, corresponding rainfall and rate of land cover change from
Landsat time-series imagery was used to discern climate versus human-induced
vegetation cover change. Spearman correlation was used to investigate the
relationship between metrics of vegetation, rainfall trends and land cover change
categories. The results show there is a positive correlation between increases in
rainfall and some land cover classes, while some classes such as settlements were
negatively correlated with vegetation productivity trends. Croplands and Natural
Vegetation were positively correlated (r=0.89) with rainfall while settlements have a
negative correlation with NDVI time series trend (r=-057). Despite the fact that
rainfall is the major determinant of vegetation cover dynamics in the study area, it
appears that other human-induced factors such as urbanization have negatively
influenced the change in vegetation cover in the study area. The results show that a
combined analysis of NDVI, rainfall and spatially explicit land cover change
provides a comprehensive insight into the drivers of vegetation cover change in
semi-arid Africa
Assessing the inter-relationship between vegetation productivity, rainfall, population and land cover over the Bani River Basin in Mali (West Africa)
This research investigated the inter-relationship between vegetation productivity, measured using
the Normalized Difference Vegetation Index (NDVI), change in rainfall and population density in the context of
perceived greening and degradation trends over the Bani River Basin (BRB). A 30-year (1982-2011), 8-km
gridded rainfall data sets was produced by inverse distance weighted (IDW) interpolation of monthly data from
40 meteorological stations contained within the basin. Population data were retrieved from the National
Population Statistic data base for 1987, 1997, and 2009. Rainfall and NDVI time-series trends were computed
for the 30-year period and analysed. The relationship between rainfall and NDVI at pixel level, and NDVI and
population densities was analysed using a Pearson correlation. Land Use and Land Cover (LULC) conversion
rates were computed for the same period using multi-temporal 30-meter Landsat imagery; ground surveys for
selected areas within the basin were used for further cross-verification. The computed NDVI trends revealed
that, vegetation 'greening' trends are mostly associated with areas where natural vegetation is still well
represented. Concurrent with increases in rainfall over the period analysed, this finding supports the hypothesis
that re-greening observed in that area is the result of multi-decadal fluctuations in climate, rather than improved
land management
The Open Global Glacier Model (OGGM) v1.1
Despite their importance for sea-level rise, seasonal water availability, and
as a source of geohazards, mountain glaciers are one of the few remaining
subsystems of the global climate system for which no globally applicable,
open source, community-driven model exists. Here we present the Open Global
Glacier Model (OGGM), developed to provide a modular and open-source
numerical model framework for simulating past and future change of any
glacier in the world. The modeling chain comprises data downloading tools
(glacier outlines, topography, climate, validation data), a preprocessing
module, a mass-balance model, a distributed ice thickness estimation model,
and an ice-flow model. The monthly mass balance is obtained from gridded
climate data and a temperature index melt model. To our knowledge, OGGM is
the first global model to explicitly simulate glacier dynamics: the model
relies on the shallow-ice approximation to compute the depth-integrated flux
of ice along multiple connected flow lines. In this paper, we describe and
illustrate each processing step by applying the model to a selection of
glaciers before running global simulations under idealized climate forcings.
Even without an in-depth calibration, the model shows very realistic
behavior. We are able to reproduce earlier estimates of global glacier volume
by varying the ice dynamical parameters within a range of plausible values.
At the same time, the increased complexity of OGGM compared to other
prevalent global glacier models comes at a reasonable computational cost:
several dozen glaciers can be simulated on a personal computer, whereas
global simulations realized in a supercomputing environment take up to a few
hours per century. Thanks to the modular framework, modules of various
complexity can be added to the code base, which allows for new kinds of model
intercomparison studies in a controlled environment. Future developments will
add new physical processes to the model as well as automated calibration
tools. Extensions or alternative parameterizations can be easily added by the
community thanks to comprehensive documentation. OGGM spans a wide range of
applications, from ice–climate interaction studies at millennial timescales
to estimates of the contribution of glaciers to past and future sea-level
change. It has the potential to become a self-sustained community-driven
model for global and regional glacier evolution.</p
Intracorporeal and Extracorporeal Anastomosis for Robotic-Assisted and Laparoscopic Right Colectomy: Short-Term Outcomes of a Multi-Center Prospective Trial
Background: Studies to date show contrasting conclusions when comparing intracorporeal and extracorporeal anastomoses for minimally invasive right colectomy. Large multi-center prospective studies comparing perioperative outcomes between these two techniques are needed. The purpose of this study was to compare intracorporeal and extracorporeal anastomoses outcomes for robotic assisted and laparoscopic right colectomy. Methods: Multi-center, prospective, observational study of patients with malignant or benign disease scheduled for laparoscopic or robotic-assisted right colectomy. Outcomes included conversion rate, gastrointestinal recovery, and complication rates. Results: There were 280 patients: 156 in the robotic assisted and laparoscopic intracorporeal anastomosis (IA) group and 124 in the robotic assisted and laparoscopic extracorporeal anastomosis (EA) group. The EA group was older (mean age 67 vs. 65 years, p = 0.05) and had fewer white (81% vs. 90%, p = 0.05) and Hispanic (2% vs. 12%, p = 0.003) patients. The EA group had more patients with comorbidities (82% vs. 72%, p = 0.04) while there was no significant difference in individual comorbidities between groups. IA was associated with fewer conversions to open and hand-assisted laparoscopic approaches (p = 0.007), shorter extraction site incision length (4.9 vs. 6.2 cm; p ≤ 0.0001), and longer operative time (156.9 vs. 118.2 min). Postoperatively, patients with IA had shorter time to first flatus, (1.5 vs. 1.8 days; p ≤ 0.0001), time to first bowel movement (1.6 vs. 2.0 days; p = 0.0005), time to resume soft/regular diet (29.0 vs. 37.5 h; p = 0.0014), and shorter length of hospital stay (median, 3 vs. 4 days; p ≤ 0.0001). Postoperative complication rates were comparable between groups. Conclusion: In this prospective, multi-center study of minimally invasive right colectomy across 20 institutions, IA was associated with significant improvements in conversion rates, return of bowel function, and shorter hospital stay, as well as significantly longer operative times compared to EA. These data validate current efforts to increase training and adoption of the IA technique for minimally invasive right colectomy
Expression, regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases
<p>Abstract</p> <p>Background</p> <p>Inflammatory lung diseases are a major morbidity factor in children. Therefore, novel strategies for early detection of inflammatory lung diseases are of high interest. Bacterial lipopolysaccharide (LPS) is recognized via Toll-like receptors and CD14. CD14 exists as a soluble (sCD14) and membrane-associated (mCD14) protein, present on the surface of leukocytes. Previous studies suggest sCD14 as potential marker for inflammatory diseases, but their potential role in pediatric lung diseases remained elusive. Therefore, we examined the expression, regulation and significance of sCD14 and mCD14 in pediatric lung diseases.</p> <p>Methods</p> <p>sCD14 levels were quantified in serum and bronchoalveolar lavage fluid (BALF) of children with infective (pneumonia, cystic fibrosis, CF) and non-infective (asthma) inflammatory lung diseases and healthy control subjects by ELISA. Membrane CD14 expression levels on monocytes in peripheral blood and on alveolar macrophages in BALF were quantified by flow cytometry. <it>In vitro </it>studies were performed to investigate which factors regulate sCD14 release and mCD14 expression.</p> <p>Results</p> <p>sCD14 serum levels were specifically increased in serum of children with pneumonia compared to CF, asthma and control subjects. <it>In vitro</it>, CpG induced the release of sCD14 levels in a protease-independent manner, whereas LPS-mediated mCD14 shedding was prevented by serine protease inhibition.</p> <p>Conclusions</p> <p>This study demonstrates for the first time the expression, regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases and suggests sCD14 as potential marker for pneumonia in children.</p
Expression, regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases
<p>Abstract</p> <p>Background</p> <p>Inflammatory lung diseases are a major morbidity factor in children. Therefore, novel strategies for early detection of inflammatory lung diseases are of high interest. Bacterial lipopolysaccharide (LPS) is recognized via Toll-like receptors and CD14. CD14 exists as a soluble (sCD14) and membrane-associated (mCD14) protein, present on the surface of leukocytes. Previous studies suggest sCD14 as potential marker for inflammatory diseases, but their potential role in pediatric lung diseases remained elusive. Therefore, we examined the expression, regulation and significance of sCD14 and mCD14 in pediatric lung diseases.</p> <p>Methods</p> <p>sCD14 levels were quantified in serum and bronchoalveolar lavage fluid (BALF) of children with infective (pneumonia, cystic fibrosis, CF) and non-infective (asthma) inflammatory lung diseases and healthy control subjects by ELISA. Membrane CD14 expression levels on monocytes in peripheral blood and on alveolar macrophages in BALF were quantified by flow cytometry. <it>In vitro </it>studies were performed to investigate which factors regulate sCD14 release and mCD14 expression.</p> <p>Results</p> <p>sCD14 serum levels were specifically increased in serum of children with pneumonia compared to CF, asthma and control subjects. <it>In vitro</it>, CpG induced the release of sCD14 levels in a protease-independent manner, whereas LPS-mediated mCD14 shedding was prevented by serine protease inhibition.</p> <p>Conclusions</p> <p>This study demonstrates for the first time the expression, regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases and suggests sCD14 as potential marker for pneumonia in children.</p
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