Coordinating Development of the SWARM-EX CubeSat Swarm Across Multiple Institutions

The Space Weather Atmospheric Reconfigurable Multiscale Experiment (SWARM-EX) is a National Science Foundation (NSF) sponsored CubeSat mission distributed across six colleges and universities in the United States. The project has three primary goals: (1) contributing to aeronomy and space weather knowledge, (2) demonstrating novel engineering technology, and (3) advancing higher education. The scientific focus of SWARM-EX is to study the spatial and temporal variability of ion-neutral interactions in the equatorial Ionosphere-Thermosphere (I-T) region. Since the mission consists of three spacecraft operating in a swarm, SWARM-EX will take in-situ measurements of the neutral and ion composition on timescales of less than an orbital period to study the persistence and correlation between different phenomena in the I-T region. The engineering objectives of SWARM-EX are focused on advancing the state of the art in spacecraft formation flying. In addition to being the first passively safe, autonomous formation of more than two spacecraft, SWARM-EX will demonstrate several other key innovations. These include a novel hybrid propulsive/differential drag control scheme and the realization of a distributed aeronomy sensor. Asa project selected by the NSF for its broader impacts as well as its intellectual merit, SWARM-EX aims to use CubeSat development as a vehicle for education. The six collaborating institutions have varying levels of CubeSat experience and involve students who range from first-year undergraduates to Ph.D. candidates. These differences in knowledge, as well as the distributed nature of the program, present a tremendous educational opportunity, but also raise challenges such as cross-institutional communication and coordination, document sharing and file management, and hardware development. By detailing its procedures for overcoming these challenges, the SWARM-EX team believes that it may serve as a case study for the coordination of a successful CubeSat program distributed across multiple institutions

Perforin Is Required for Innate and Adaptive Immunity Induced by Heat Shock Protein Gp96

Tumor-secreted gp96-Ig is highly immunogenic and triggers CD8 T cell-mediated tumor rejection. In vivo secreted gp96-Ig and gp96-myc cause NK activation and clonal expansion of specific CD8+ CTL in wild-type and in Fas-ligand-deficient (gld) mice but not in perforin- (PKO) or IFN-γ-deficient (GKO) mice. Transfer of perforin-competent NK cells restores the ability of PKO mice to clonally expand CD8 CTL in response to gp96-Ig. The data demonstrate an essential role for perforin-mediated functions in the activation of innate and adaptive immunity by heat shock protein gp96-peptide complexes. Crosspresentation of antigens by heat shock proteins seems to require a perforin-dependent positive feedback loop between NK and DC for both sustained NK activation and clonal CTL expansion. The studies also explain how depressed NK activity in patients with tumors or after viral infections could diminish CTL responses