Operational characteristic, indicators, and data sources for evaluation.

<p>Operational characteristic, indicators, and data sources for evaluation.</p

Major concerns and recommendations and/or actions taken to improve the Ebola Virus Disease Rapid Diagnostic Testing program.

<p>Major concerns and recommendations and/or actions taken to improve the Ebola Virus Disease Rapid Diagnostic Testing program.</p

Concordance between OraQuick^® Ebola Rapid Antigen Test and PCR testing for living patients and the deceased from October 2015 to April 2016 in Guinea.

<p>Concordance between OraQuick^® Ebola Rapid Antigen Test and PCR testing for living patients and the deceased from October 2015 to April 2016 in Guinea.</p

Knowledge retention of Ebola Virus Disease Rapid Diagnostic Testing.

<p>Maximum score is 8. Respondent performance is shown overall, by region, by professional status, and by training type received.</p

Percentage of laboratories with equipment for the EVD RDT program by region.

<p>Percentage of laboratories with equipment for the EVD RDT program by region.</p

Cumulated cholera attack rates and deaths per prefecture during the 2012 Guinean epidemic.

<p>Cumulated cholera attack rates and deaths per prefecture during the 2012 Guinean epidemic.</p

Cholera in Maritime Guinea between February and May 2012.

<p>The map illustrates the early propagation of the outbreak along the coast and the cumulated attack rate per sub-prefecture. Village positions are available on the Index Mundi website (<a href="http://www.indexmundi.com/zp/gv/" target="_blank">http://www.indexmundi.com/zp/gv/</a>).</p

MLVA-based genotypes and relatedness of 38 clinical <i>V. cholerae</i> strains isolated in Guinea in 2012.

<p>(A) Network of <i>V. cholerae</i> strain relatedness based on MLVA genotype. Following the genotype analysis of 38 <i>V. cholerae</i> strains using 6 different microsatellite loci, the strains were grouped according to the resulting MLVA genotype profiles. Each colored circle corresponds to a different genotype. The numbers indicate the sequential order when the first strain of the corresponding genotype was isolated. Circle diameter is relatively proportional to the number of isolates represented by each genotype (e.g., 14 strains displayed genotype #1, 7 isolates displayed genotype #8 and 1 strain displayed genotype #3). Each segment corresponds to a single mutation at 1 of the 6 assessed VNTRs. Bold segments represent primary and likely relatedness links between genotypes, while the dashed segments represent secondary and less likely genetic relationships. Genotype #1 was identified as the founder genotype using the eBURST algorithm. (B) Spatiotemporal repartition of genotyped <i>V. cholerae</i> strains. Prefecture-level maps of Guinea are displayed for each month from February to September 2012. The genotype color code described in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002898#pntd-0002898-g004" target="_blank">Figure 4A</a> was applied to spatially and temporarily localize the isolated strains. Therefore, the pie charts reflect the month and prefecture of strain isolation (represented by strain genotype) as well as the relative proportion of each genotype among them. Segments between different months spatially and temporarily illustrate the genetic relatedness displayed in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002898#pntd-0002898-g004" target="_blank">Figure 4A</a>. Only primary and sequentially earliest links between genotypes are represented.</p

(A) Maximum likelihood phylogenetic tree of the seventh pandemic lineage of <i>V. cholerae</i> based on the SNP differences across the whole core genome and including a strain isolated during the onset of the Guinean 2012 outbreak.

<p>The pre-seventh pandemic isolate M66 was used as an outgroup to root the tree. Blue, green and red branches represent waves 1, 2 and 3, respectively. Purple and sky blue clade lineages represent the Kenyan clade and two South Asian clades within the third wave, respectively. Scale is provided as the number of substitutions per variable site. (B) Greater resolution of wave 3 of the seventh pandemic, in which the Guinean strain clustered distinctly from the two South Asian clades and the dominant Kenyan clade. Guinean isolate G298 is represented by the square while each colored circle indicates a spatially different isolate (as shown in the key). Scale is provided as the number of substitutions per variable site.</p

The Ebola virus disease epidemic in the Conakry area, Guinea, March 2014 to February 2015.

<p>(A) Map of the study area, which consists of Conakry and the surrounding prefectures of Boffa, Coyah, Dubreka, Forecariah, Fria, Kindia, and Telimele (for which diagnoses were mostly performed by the IPD-LFHP laboratory) (the administrative boundaries were taken from the GADM database; <a href="http://www.gadm.org/" target="_blank">http://www.gadm.org/</a>). (B) Number of cases by month of symptom onset. The total number of probable and confirmed cases in the study area that were hospitalized is indicated in grey. The number of those that were diagnosed by reverse transcription PCR (RT-PCR) by the IPD-LFHP laboratory is in blue.</p