Primers used for PCR detection of genes encoding virulence factors.

<p>Other virulence factors have been detected by multiplex PCR onto purified total DNA (Qiagen). Presence of genes encoding enterotoxins E, G, H, K, L, T, epidermolysin D (Etd), genes encoding Edin A and EDIN B and the genes encoding seven adhsesion factors (Cna, FnbA, FnbB Bbp, Clfb, Fib, Ebp and Lbp) was checked in 8 set in function of base size.</p

Pulsed field gel electrophoresis (PFGE) dependent dendogram of isolated <i>Staphylococcus aureus</i>.

<p>Pulsed field gel electrophoresis (PFGE) proves no specific clonal relationship between PVL-producing isolates issued from furuncles or secondary dermatosis. The similarity of the different pulsotypes was established by using Molecular Analyst™ software. Twenty four pulsotypes corresponded to the 55 isolates and their distribution is given according to the groups of isolates issued from secondary infected dermatosis (1), furuncles from HIV (−)(2) or HIV (+)(3) patients.</p

Production of toxins and identification of genes encoding toxins in <i>Staphylococcus aureus</i>.

<p>The majority of <i>SA</i> strains isolated from HIV patient-derived furuncles significantly produced PVL (<i>p</i><0.05), whereas only 10% of <i>SA</i> strains produced this toxin in secondary infected dermatosis. A high prevalence of LukE-LukD-producing isolates (56 to 78%) was recorded in patient groups.</p

Clinical signs reported before start of antibiotics for a suspected secondary infected BU.

<p>WBC = White Blood cell Count.</p

Number of days of prescribed antibiotics per 100 days in hospital.

*<p>Standard treatment of <i>M. ulcerans</i> with 56 days of rifampicin and streptomycin not included in this table.</p

Suggested guideline for use of antibiotics in prophylaxis and suspected secondary infections.

<p>* Ciprofloxacin is not generally recommended for use in children, but appears relatively safe <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002010#pntd.0002010-Grayson1" target="_blank">[36]</a>.</p

Susceptibility of cultured organisms (other than <i>M. ulcerans</i>) in Buruli ulcer lesions in Benin.

<p>Cultures taken from both the center and the border of the ulcer at start of treatment in patients followed longitudinally were included, leading to a different number of cultures than in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002010#pntd-0002010-t004" target="_blank">Table 4</a>.</p

Antibiotic treatment given to patients for different Buruli ulcer related indications.

<p>Antibiotic treatment given to patients for different Buruli ulcer related indications.</p

Results of cultures in 71 patients before, during or after treatment with streptomycin and rifampicin.

<p>For patients followed longitudinally, only cultures from the center of the ulcer were included at inclusion (cultures from border gave similar results).</p

Sensitivity of the cultured MRSA.

<p>Sensitivity of the cultured MRSA.</p