Effect of tempol on cisplatin-induced changes in oxidative stress markers in postmitochondrial and mitochondrial fractions in renal tissues of mice.

<p>(A) Reduced glutathione (GSH). (B) Thiobarbituric acid reactive substances (TBARS). Mice were studied 72 h after a single i.p. injection of cisplatin (25 mg/kg). Tempol (100 mg/kg/day) was given orally for 4 days starting one day before cisplatin injection. Each value represents the mean of 6–8 mice ± S.E.M. *<i>p<0.05 vs.</i> normal, ^#<i>p<0.05 vs</i>. cisplatin.</p

Effect of tempol on cisplatin-induced changes in mitochondrial ultrastructural examination of renal tissues in mice.

<p>Photomicrographs are representative specimens which show mitochondria (m) and cytoplasm (c) from normal group (A x2,000 magnification; B x10,000 magnification), tempol-treated group (C x2,000 magnification; D x10,000 magnification), cisplatin-treated group (E x2,000 magnification; F x10,000 magnification), tempol and cisplatin-treated group (G x2,000 magnification; H x10,000 magnification), mitochondrial overall injury score (I) and percentage of mitochondrial cross-sectional area/cytoplasmic area (J). Mice were studied 72 h after a single i.p. injection of cisplatin (25 mg/kg). Tempol (100 mg/kg/day) was given orally for 4 days starting one day before cisplatin injection. Each value represents the mean of 4 mice ± S.E.M. *<i>p<0.05 vs.</i> normal, ^#<i>p<0.05 vs</i>. cisplatin.</p

Effect of tempol on cisplatin-induced changes in light microscopic examination (H&E x200) in renal tissues of mice.

<p>(A) normal group and (B) tempol-treated group show normal non-affected tubular epithelium (thick dark arrow). (C) cisplatin-treated group shows severe tubular damage as revealed by acute tubular necrosis (dashed thick arrow), wide tubular epithelial vacuolation (v), apoptotic tubular epithelium (thick white arrow) and cast formation (s). (D) tempol and cisplatin-treated group shows more or less normal renal tubules with minimal focal vacuolation of the tubular epithelium (v). (E) renal injury score. Mice were studied 72 h after a single i.p. injection of cisplatin (25 mg/kg). Tempol (100 mg/kg/day) was given orally for 4 days starting one day before cisplatin injection. Each renal injury score value represents the mean of 4 mice ± S.E.M. *<i>p<0.05 vs.</i> normal, ^#<i>p<0.05 vs</i>. cisplatin.</p

Effect of tempol on cisplatin-induced changes in antioxidant enzymes activities in postmitochondrial and mitochondrial fractions in renal tissues of mice.

<p>(A) Superoxide dismutase (SOD). (B) Catalase. Mice were studied 72 h after a single i.p. injection of cisplatin (25 mg/kg). Tempol (100 mg/kg/day) was given orally for 4 days starting one day before cisplatin injection. Each value represents the mean of 6–8 mice ± S.E.M. *<i>p<0.05 vs.</i> normal, ^#<i>p<0.05 vs</i>. cisplatin.</p

Effect of tempol, cisplatin and their combination on the growth of solid Ehrlich carcinoma 72 h after starting treatment.

<p>Mice with a palpable solid tumor mass (100 mm³) were given a single i.p. injection of cisplatin (25 mg/kg) or tempol orally in a dose of 100 mg/kg/day or a combination of tempol and cisplatin. The change in tumor volume was measured after 3 days using a Vernier caliper. Each value represents the mean of 5–6 mice ± S.E.M. ^$<i>p<0.05 vs</i>. control.</p><p>Effect of tempol, cisplatin and their combination on the growth of solid Ehrlich carcinoma 72 h after starting treatment.</p

Effect of tempol on cisplatin-induced changes in mitochondrial complexes I–IVactivities, oxidative phosphorylation capacity and adenosine triphosphate (ATP) content in renal tissues of mice.

<p>Mice were studied 72 h after a single i.p. injection of cisplatin (25 mg/kg). Tempol (100 mg/kg/day) was given orally for 4 days starting one day before cisplatin injection. Each value represents the mean of 6–8 mice ± S.E.M. *<i>p<0.05 vs.</i> normal, ^#<i>p<0.05 vs</i>. cisplatin.</p><p>Effect of tempol on cisplatin-induced changes in mitochondrial complexes I–IVactivities, oxidative phosphorylation capacity and adenosine triphosphate (ATP) content in renal tissues of mice.</p