3D Image quality improvement for optical projection tomography via point spread function modelling

Microbial Biotechnolog

Simultaneous imaging of Pseudomonas fluorescens WCS365 populations expressing three different autofluorescent proteins in the rhizosphere: New perspectives for studying microbial communities

Microbial Biotechnolog

Membrane Fusion Mediated Intracellular Delivery of Lipid Bilayer Coated Mesoporous Silica Nanoparticles

Supramolecular & Biomaterials Chemistr

14-3-3 isoforms and pattern formation during barley microspore embryogenesis

Plant science

Role of chemotaxis toward fusaric acid in colonization of hyphae of Fusarium oxysporum f.sp. radicis-lycopersici by Pseudomonas fluorescens WCS365

Microbial Biotechnolog

Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88

Animal science

Elucidation of the serosal cuticle machinery in the beetle Tribolium by RNA sequencing and functional analysis of Knickkopf1, Retroactive and Laccase2

Animal science

Embedding root and nodule tissue in plastic (BMM)

Immunolocalisation and in situ hybridisation allow the detection of proteins and RNA respectively, in individual cells of different tissue types. Hybridisation on serial 5–8 μm sections is most frequently performed with tissue embedded in paraffin wax but it is often difficult to obtain high-resolution sections from soft tissue with this embedding process. To overcome this, alternative localisation protocols have been developed utilising plastic resins. We have used plastic embedded tissue from Lotus japonicus roots and young nodules successfully in immunolocalisation experiments and developed a protocol that can also be adapted for in situ RNA localisation studies. The different parameters tested are described, as well as the use of alkaline phosphatase- or fluorescently-conjugated secondary antibodies.Microbial Biotechnolog