Programmable multimetallic linear nanoassemblies of ruthenium–DNA conjugates

A new ruthenium–DNA conjugates family was synthesized, made up of a ruthenium complex bound to one or two identical DNA strands of 14–58 nucleotides. The formation of controlled linear nanoassemblies containing one to seven ruthenium complexes is described

Correlations between electrochemical behaviors and DNA photooxidative properties of non-steroïdal anti-inflammatory drugs and their photoproducts

Alkali-labile lesion to DNA photosensitized, via an electron transfer mechanism, by three non-steroidalanti-inflammatorydrugs (NSAIDs), ketoprofen, tiaprofenic acid and naproxen and their photoproducts during drug photolysis, was investigated using 32P-end labelled synthetic oligonucleotide. These photooxidative damages were correlated with the photophysical and electrochemicalproperties of drugs, appearing as the photosensitizer PS. Photophysical studies provided the excited state energies of the photosensitizer while their redox potentials and the relative stabilities of the PSradical dot− radical-anions were determined by cyclic voltammetry. On the basis of these data, we have calculated the Gibbs energy of photoinduced electron-transfer and evaluated the exergonicity of the oxidative photodamage. Moreover, kinetic control may be invoked according to the stabilities of PSradical dot−. Applied to this NSAIDs family, the photoxidative damages through electron transfer mechanism were analyzed and a good correlation with photoredox and photobiological properties was established

DNA three-way junction-ruthenium complex assemblies

Three-way junction building blocks were designed to construct novel 2D ruthenium-DNA assemblies. Discrete three - branched DNA motifs were formed with 1 to 3 sticky ends of 14-, 20- and/or 24-mer nucleotides. Hybridization with the complementary mono Ru-DNA conjugates afforded the formation of a family of three-way assemblies with 1 to 3 peripheral ruthenium complexes. The use of sticky ends of different lengths allowed us to modulate the number of metallic complexes introduced and also to extend the structure. Indeed, our construction strategy associated with the use of bis ruthenium-DNA conjugates permitted the assembly of double three-way junctions linked by DNA duplexes with or without ruthenium complxes in their centre

A large scale hearing loss screen reveals an extensive unexplored genetic landscape for auditory dysfunction

The developmental and physiological complexity of the auditory system is likely reflected in the underlying set of genes involved in auditory function. In humans, over 150 non-syndromic loci have been identified, and there are more than 400 human genetic syndromes with a hearing loss component. Over 100 non-syndromic hearing loss genes have been identified in mouse and human, but we remain ignorant of the full extent of the genetic landscape involved in auditory dysfunction. As part of the International Mouse Phenotyping Consortium, we undertook a hearing loss screen in a cohort of 3006 mouse knockout strains. In total, we identify 67 candidate hearing loss genes. We detect known hearing loss genes, but the vast majority, 52, of the candidate genes were novel. Our analysis reveals a large and unexplored genetic landscape involved with auditory function

Nat Genet

The function of the majority of genes in the mouse and human genomes remains unknown. The mouse embryonic stem cell knockout resource provides a basis for the characterization of relationships between genes and phenotypes. The EUMODIC consortium developed and validated robust methodologies for the broad-based phenotyping of knockouts through a pipeline comprising 20 disease-oriented platforms. We developed new statistical methods for pipeline design and data analysis aimed at detecting reproducible phenotypes with high power. We acquired phenotype data from 449 mutant alleles, representing 320 unique genes, of which half had no previous functional annotation. We captured data from over 27,000 mice, finding that 83% of the mutant lines are phenodeviant, with 65% demonstrating pleiotropy. Surprisingly, we found significant differences in phenotype annotation according to zygosity. New phenotypes were uncovered for many genes with previously unknown function, providing a powerful basis for hypothesis generation and further investigation in diverse systems.Comment in : Genetic differential calculus. [Nat Genet. 2015] Comment in : Scaling up phenotyping studies. [Nat Biotechnol. 2015

Simulation of deep bed dryers : application to the drying of rice in Iran

Simulation of deep bed dryers : application to the drying of rice in Iran. 9. International Conference on Engineering and Food (ICEF9), March 7-1

Multivalent Azide-Functionalized Polypyridyl Ruthenium Complexes and Their DNA Conjugates through Click Chemistry

International audienc

Formulation induces direct DNA UVA photooxidation. Part I. Role of the formulating cationic surfactant

International audienc

Electrocarboxylation of chloroacetonitrile mediated by a Ni(I) terpyridine complex: Voltammetric and spectroscopic studies

International audienc

Formulation induces direct DNA UV-A photooxidation. Part II. Pro-oxidant effect of formulated Vitamin E via generation of singlet oxygen

International audienceHIGHLIGHTS · Cetyltriethylammonium bromide (CTEAB) and Vitamin E self-assemble in TRIS/HCl buffer solutions into nanoemulsion composed of CTEAB micelles (that can incorporate Vitamin E) as well as larger Vitamin E droplets. · CTEAB-Vitamin E nanoemulsion interacts with plasmid DNA pBR322 and the gradual DNA compaction followed by gradual DNA decompaction for increasing CTEAB-Vitamin E amounts have been highlighted. · UVA irradiation induces photooxidation of DNA on the formulated CTEAB-Vitamin E-DNA lipoplex and underlines the pro-oxidant role of the Vitamin E in this self-assembled system