DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING RP-UPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TEZACAFTOR AND IVACAFTOR IN FORMULATIONS

Objective: Aim of the present research work was to develop a sensitive, rapid and accurate, stability-indicating RP-UPLC method for the simultaneous estimation of tezacaftor and ivacaftor in formulations. Methods: The chromatographic separation of the mixture of tezacaftor and ivacaftor was attained in isocratic method utilizing a mobile phase of 0.1 % orthophosphoric acid and acetonitrile in the proportion of 50:50%v/v utilizing a HSS C18 column which has dimensions of 100×2.1 mm, 1.7 m particle size and the flow rate of 0.3 ml/min. The detection system was monitored at 292 nm wavelength maximum with 1.5 ml injection volume. The present method was validated as per the guidelines given by the ICH for specificity, accuracy, sensitivity, linearity and precision. Results: The retaining time for tezacaftor and ivacaftor were achieved at 1.071 min and 0.530 min, respectively. Tezacaftor, ivacaftor and their combined drug formulation were exposed to thermal, acidic, oxidative, photolytic, and alkaline conditions. The developed method was highly sensitive, rapid, precise and accurate than the earlier reported methods. The total run time was decreased to 2.0 min; hence, the technique was more precise and economical. Stability studies directed for the suitability of the technique for degradation studies of tezacaftor and ivacaftor. Conclusion: The projected method can be utilized for routine analysis in the quality control department in pharmaceutical trades

Solitons in one-dimensional interacting Bose-Einstein system

A modified Gross-Pitaevskii approximation was introduced recently for bosons in dimension $d\le2$ by Kolomeisky {\it et al.} (Phys. Rev. Lett. {\bf 85} 1146 (2000)). We use the density functional approach with sixth-degree interaction energy term in the Bose field to reproduce the stationary-frame results of Kolomeisky {\it et al.} for a one-dimensional Bose-Einstein system with a repulsive interaction. We also find a soliton solution for an attractive interaction, which may be boosted to a finite velocity by a Galilean transformation. The stability of such a soliton is discussed analytically. We provide a general treatment of stationary solutions in one dimension which includes the above solutions as special cases. This treatment leads to a variety of stationary wave solutions for both attractive and repulsive interactions.Comment: Latex, 14 pages, No figur

VBN 3: A new high yielding multiple disease resistant cowpea variety

Cowpea variety VBN 3 (VCP 09-013) is derived from TLS 38 x VCP 16-1. The average yield of VBN 3 is 1013 kg / ha. It is a 17.0 and 17.2 per cent yield increase over VBN 1 (866 kg/ha) and CO (CP) 7 (864 kg/ha), respectively. Duration is 75 – 80 days. It recorded the grain yield of 1148 kg/ha under the irrigated condition which is a 13.8 and 18.0 per cent yield increase over the check varieties VBN 1 (1009 kg/ha) and CO (CP) 7 (973 kg/ ha), respectively. In rainfed conditions, this variety recorded the seed yield of 1013 kg/ha. It is a 17.0 and 17.2 per cent yield increase over the check varieties VBN 1 (866 kg/ha) and CO (CP) 7 (864 kg/ha, respectively. It is having the special features of determinate plant type, synchronized maturity, multiple resistance to bean common mosaic virus, rust and anthracnose diseases. It is resistant to pod borer and pod bug. The cowpea VBN3 is having a protein content of 25.22 per cent. It recorded 100 grain weight of 13.0 g with preferable brown seed colour. It is suitable for cultivation during Rabi season (Purattasi pattam) in Tamil Nadu

Exchange bias effect in alloys and compounds

The phenomenology of exchange bias effects observed in structurally single-phase alloys and compounds but composed of a variety of coexisting magnetic phases such as ferromagnetic, antiferromagnetic, ferrimagnetic, spin-glass, cluster-glass and disordered magnetic states are reviewed. The investigations on exchange bias effects are discussed in diverse types of alloys and compounds where qualitative and quantitative aspects of magnetism are focused based on macroscopic experimental tools such as magnetization and magnetoresistance measurements. Here, we focus on improvement of fundamental issues of the exchange bias effects rather than on their technological importance

Marine Ecosystem Challenges & Opportunities (MECOS 3)

The Marine Biological Association of India (MBAI), established in 1958, is proud to gear up for MECOS3, the third symposium on Marine Ecosystems- Challenges and Opportunities during 7-10 January, 2020. The MBAI besides organising MECOS1 (2009) and MECOS2 (2014) has inculcated active interest and participation among its members by handling several national and international symposia/seminars, since its formation. The MBAI has 794 life members and 20 institutional members. The mandate of the MBAI is promotion of scientific research in the field of marine biology and allied sciences

Cyanobacteria of the thermal spring at Pancharevo, Sofia, Bulgaria

Eight taxa of cyanobacteria were identified in the thermal spring at Pancharevo (in the Sofia basin, Bulgaria). As well as the widespread Lyngbya thermalis, Phormidesmis molle (syn. Phormidium molle), Phormidium papyraceum, Phormidium corium and Mastigocladus laminosus, four species were identified for the first time in Bulgaria: Calothrix thermalis, Gloeocapsa gelatinosa, Leibleinia epiphytica and Symploca thermalis

Physics Potential of the ICAL detector at the India-based Neutrino Observatory (INO)

The upcoming 50 kt magnetized iron calorimeter (ICAL) detector at the India-based Neutrino Observatory (INO) is designed to study the atmospheric neutrinos and antineutrinos separately over a wide range of energies and path lengths. The primary focus of this experiment is to explore the Earth matter effects by observing the energy and zenith angle dependence of the atmospheric neutrinos in the multi-GeV range. This study will be crucial to address some of the outstanding issues in neutrino oscillation physics, including the fundamental issue of neutrino mass hierarchy. In this document, we present the physics potential of the detector as obtained from realistic detector simulations. We describe the simulation framework, the neutrino interactions in the detector, and the expected response of the detector to particles traversing it. The ICAL detector can determine the energy and direction of the muons to a high precision, and in addition, its sensitivity to multi-GeV hadrons increases its physics reach substantially. Its charge identification capability, and hence its ability to distinguish neutrinos from antineutrinos, makes it an efficient detector for determining the neutrino mass hierarchy. In this report, we outline the analyses carried out for the determination of neutrino mass hierarchy and precision measurements of atmospheric neutrino mixing parameters at ICAL, and give the expected physics reach of the detector with 10 years of runtime. We also explore the potential of ICAL for probing new physics scenarios like CPT violation and the presence of magnetic monopoles.Comment: 139 pages, Physics White Paper of the ICAL (INO) Collaboration, Contents identical with the version published in Pramana - J. Physic

Multiple Genome Wide Association Mapping Models Identify Quantitative Trait Nucleotides for Brown Planthopper (Nilaparvata lugens) Resistance in MAGIC Indica Population of Rice

Brown planthopper (BPH), one of the most important pests of the rice (Oryza sativa) crop, becomes catastrophic under severe infestations and causes up to 60% yield loss. The highly disastrous BPH biotype in the Indian sub-continent is Biotype 4, which also known as the South Asian Biotype. Though many resistance genes were mapped until now, the utility of the resistance genes in the breeding programs is limited due to the breakdown of resistance and emergence of new biotypes. Hence, to identify the resistance genes for this economically important pest, we have used a multi-parent advanced generation intercross (MAGIC) panel consisting of 391 lines developed from eight indica founder parents. The panel was phenotyped at the controlled conditions for two consecutive years. A set of 27,041 cured polymorphic single nucleotide polymorphism (SNPs) and across-year phenotypic data were used for the identification of marker–trait associations. Genome-wide association analysis was performed to find out consistent associations by employing four single and two multi-locus models. Sixty-one SNPs were consistently detected by all six models. A set of 190 significant marker-associations identified by fixed and random model circulating probability unification (FarmCPU) were considered for searching resistance candidate genes. The highest number of annotated genes were found in chromosome 6 followed by 5 and 1. Ninety-two annotated genes identified across chromosomes of which 13 genes are associated BPH resistance including NB-ARC (nucleotide binding in APAF-1, R gene products, and CED-4) domain-containing protein, NHL repeat-containing protein, LRR containing protein, and WRKY70. The significant SNPs and resistant lines identified from our study could be used for an accelerated breeding program to develop new BPH resistant cultivars

Evidence-based national vaccine policy

India has over a century old tradition of development and production of vaccines. The Government rightly adopted self-sufficiency in vaccine production and self-reliance in vaccine technology as its policy objectives in 1986. However, in the absence of a full-fledged vaccine policy, there have been concerns related to demand and supply, manufacture vs. import, role of public and private sectors, choice of vaccines, new and combination vaccines, universal vs. selective vaccination, routine immunization vs. special drives, cost-benefit aspects, regulatory issues, logistics etc. The need for a comprehensive and evidence based vaccine policy that enables informed decisions on all these aspects from the public health point of view brought together doctors, scientists, policy analysts, lawyers and civil society representatives to formulate this policy paper for the consideration of the Government. This paper evolved out of the first ever ICMR-NISTADS national brainstorming workshop on vaccine policy held during 4-5 June, 2009 in New Delhi, and subsequent discussions over email for several weeks, before being adopted unanimously in the present form