What can be learnt from a highly informative X-ray occultation event in NGC 6814? A marvellous absorber

A unique X-ray occultation event in NGC 6814 during an XMM-Newton observation in 2016 has been reported, providing useful information of the absorber and the corona. We revisit the event with the aid of the hardness ratio (HR) - count rate (CR) plot and comparison with two other absorption-free XMM exposures in 2009 and 2021. NGC 6814 exhibits a clear "softer-when-brighter" variation pattern during the exposures, but the 2016 exposure significantly deviates from the other two in the HR - CR plot. While spectral fitting does yield transient Compton-thin absorption corresponding to the eclipse event in 2016, rather than easing the tension between exposures in the HR - CR plot, correcting the transient Compton-thin absorption results in new and severe deviation within the 2016 exposure. We show that the eclipsing absorber shall be clumpy (instead of a single Compton-thin cloud), with an inner denser region composed of both Compton-thin and Compton-thick clouds responsible for the previously identified occultation event, and an outer sparser region with Compton-thin clouds which eclipses the whole 2016 exposure. With this model, all the tension in the HR - CR plots could be naturally erased, with the observed spectral variability during the 2016 exposure dominated by the variation of absorption. Furthermore, the two warm absorbers (with different ionization and column densities but similar outflowing velocities) detected in the 2016 exposure shall also associate with the transient absorber, likely due to ablated or tidal stretched/disrupted fragments. This work highlights the unique usefulness of the HR - CR plot while analysing rare occultation events.Comment: 13 pages, 9 figures, accepted by MNRAS. For the video of the eclipsing cloud, see https://drive.google.com/file/d/1CEsPEWE-b5W8PfZINyI5K1sE6klHaYMa/view?usp=sharing . Comments are welcome

Cloud Based Data Protection in Anonymously Controlled SDN

Nowadays, Software Defined Network (SDN) develops rapidly for its novel structure which separates the control plane and the data plane of network devices. Many researchers devoted themselves to the study of such a special network. However, some limitations restrict the development of SDN. On the one hand, the single controller in the conventional model bears all threats, and the corruption of it will result in network paralysis. On the other hand, the data will be increasing more in SDN switches in the data plane, while the storage space of these switches is limited. In order to solve the mentioned issues, we propose two corresponding protocols in this paper. Specifically, one is an anonymous protocol in the control plane, and the other is a verifiable outsourcing protocol in the data plane. The evaluation indicates that our protocol is correct, secure, and efficient

PinX1 regulation of telomerase activity and apoptosis in nasopharyngeal carcinoma cells

<p>Abstract</p> <p>Background</p> <p>Human interacting protein X1 (PinX1) has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, however, its function in inhibiting telomerase activity of tumor cells is not well documented. Here we show that PinX1 is essential for down-regulation telomerase activity of nasopharyngeal carcinoma.</p> <p>Methods</p> <p>Expression vectors of human PinX1 (pEGFP-C3-PinX1) and its small interfering RNA (PinX1-FAM-siRNA) were constructed and transfected into NPC. Their effects on mRNA of telomerase catalytic subunit (hTERT), telomerase activity, cell proliferation, cell migration, wound healing, cell cycles and apoptosis were examined using semi-quantitative RT-PCR, stretch PCR, MTT assay, Transwell, scratch assay and flow cytometry, respectively.</p> <p>Results</p> <p>Transfection of pEGFP-C3-PinX1 and PinX1-FAM-siRNA increased and reduced PinX1 mRNA by 1.6-fold and 70%, respectively. Over-expression of PinX1 decreased hTERT mRNA by 21%, reduced telomerase activity, inhibited cell growth, migration and wound healing ability, arrested cells in G0/G1 phase, and increased apoptotic index. In contrast, down-regulation of PinX1 did not alter the above characteristics.</p> <p>Conclusions</p> <p>PinX1 may play important roles in NPC proliferation, migration and apoptosis and has application potential in tumor-targeted gene therapy.</p

Comparative evaluation of the diagnosis, reporting and investigation of malaria cases in China, 2005-2014: transition from control to elimination for the national malaria programme

Background: The elimination of malaria requires high-quality surveillance data to enable rapid detection and response to individual cases. Evaluation of the performance of a national malaria surveillance system could identify shortcomings which, if addressed, will improve the surveillance program for malaria elimination.Methods: Case-level data for the period 2005–2014 were extracted from the China National Notifiable Infectious Disease Reporting Information System and Malaria Enhanced Surveillance Information System. The occurrence of cases, accuracy and timeliness of case diagnosis, reporting and investigation, were assessed and compared between the malaria control stage (2005–2010) and elimination stage (2011–2014) in mainland China.Results: A total of 210 730 malaria cases were reported in mainland China in 2005–2014. The average annual incidence declined dramatically from 2.5 per 100 000 people at the control stage to 0.2 per 100 000 at the elimination stage, but the proportion of migrant cases increased from 9.8 % to 41.0 %. Since the initiation of the National Malaria Elimination Programme in 2010, the overall proportion of cases diagnosed by laboratory testing consistently improved, with the highest of 99.0 % in 2014. However, this proportion was significantly lower in non-endemic provinces (79.0 %) than that in endemic provinces (91.4 %) during 2011–2014. The median interval from illness onset to diagnosis was 3 days at the elimination stage, with one day earlier than that at the control stage. Since 2011, more than 99 % cases were reported within 1 day after being diagnosed, while the proportion of cases that were reported within one day after diagnosis was lowest in Tibet (37.5 %). The predominant source of cases reporting shifted from town-level hospitals at the control stage (67.9 % cases) to city-level hospitals and public health institutes at the eliminate stage (69.4 % cases). The proportion of investigation within 3 days after case reporting has improved, from 74.6 % in 2010 to 98.5 % in 2014.Conclusions: The individual case-based malaria surveillance system in China operated well during the malaria elimination stage. This ensured that malaria cases could be diagnosed, reported and timely investigated at local level. However, domestic migrants and overseas populations, as well as cases in the historically malarial non-endemic areas and hard-to-reach area are new challenges in the surveillance for malaria elimination.<br/

Epigenetic control of translation checkpoint and tumor progression via RUVBL1-EEF1A1 axis

Epigenetic dysregulation is reported in multiple cancers including Ewing sarcoma (EwS). However, the epigenetic networks underlying the maintenance of oncogenic signaling and therapeutic response remain unclear. Using a series of epigenetics- and complex-focused CRISPR screens, RUVBL1, the ATPase component of NuA4 histone acetyltransferase complex, is identified to be essential for EwS tumor progression. Suppression of RUVBL1 leads to attenuated tumor growth, loss of histone H4 acetylation, and ablated MYC signaling. Mechanistically, RUVBL1 controls MYC chromatin binding and modulates the MYC-driven EEF1A1 expression and thus protein synthesis. High-density CRISPR gene body scan pinpoints the critical MYC interacting residue in RUVBL1. Finally, this study reveals the synergism between RUVBL1 suppression and pharmacological inhibition of MYC in EwS xenografts and patient-derived samples. These results indicate that the dynamic interplay between chromatin remodelers, oncogenic transcription factors, and protein translation machinery can provide novel opportunities for combination cancer therapy.</p

Contactless multiscale measurement of cardiac motion using biomedical radar sensor

IntroductionA contactless multiscale cardiac motion measurement method is proposed using impulse radio ultra-wideband (IR-UWB) radar at a center frequency of 7.29 GHz.MotivationElectrocardiograph (ECG), heart sound, and ultrasound are traditional state-of-the-art heartbeat signal measurement methods. These methods suffer from defects in contact and the existence of a blind information segment during the cardiogram measurement.MethodsExperiments and analyses were conducted using coarse-to-fine scale. Anteroposterior and along-the-arc measurements were taken from five healthy male subjects (aged 25–43) when lying down or prone. In every measurement, 10 seconds of breath-holding data were recorded with a radar 55 cm away from the body surface, while the ECG was monitored simultaneously as a reference.ResultsCardiac motion detection from the front was superior to that from the back in amplitude. In terms of radar detection angles, the best cardiac motion information was observed at a detection angle of 120°. Finally, in terms of cardiac motion cycles, all the ECG information, as well as short segments of cardiac motion details named blind ECGs segments, were detected.SignificanceA contactless and multiscale cardiac motion detection method is proposed with no blind detection of segments during the entire cardiac cycle. This paves the way for a potentially significant method of fast and accurate cardiac disease assessment and diagnosis that exhibits promising application prospects in contactless online cardiac monitoring and in-home healthcare

Application and comparison of Kalman filters for coastal ocean problems : an experiment with FVCOM

Author Posting. © American Geophysical Union, 2009. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 114 (2009): C05011, doi:10.1029/2007JC004548.Twin experiments were made to compare the reduced rank Kalman filter (RRKF), ensemble Kalman filter (EnKF), and ensemble square-root Kalman filter (EnSKF) for coastal ocean problems in three idealized regimes: a flat bottom circular shelf driven by tidal forcing at the open boundary; an linear slope continental shelf with river discharge; and a rectangular estuary with tidal flushing intertidal zones and freshwater discharge. The hydrodynamics model used in this study is the unstructured grid Finite-Volume Coastal Ocean Model (FVCOM). Comparison results show that the success of the data assimilation method depends on sampling location, assimilation methods (univariate or multivariate covariance approaches), and the nature of the dynamical system. In general, for these applications, EnKF and EnSKF work better than RRKF, especially for time-dependent cases with large perturbations. In EnKF and EnSKF, multivariate covariance approaches should be used in assimilation to avoid the appearance of unrealistic numerical oscillations. Because the coastal ocean features multiscale dynamics in time and space, a case-by-case approach should be used to determine the most effective and most reliable data assimilation method for different dynamical systems.P. Malanotte-Rizzoli and J. Wei were supported by the Office of Naval Research (ONR grant N00014-06-1- 0290); C. Chen and Q. Xu were supported by the U.S. GLOBEC/Georges Bank program (through NSF grants OCE-0234545, OCE-0227679, OCE- 0606928, OCE-0712903, OCE-0726851, and OCE-0814505 and NOAA grant NA-16OP2323), the NSF Arctic research grants ARC0712903, ARC0732084, and ARC0804029, and URI Sea Grant R/P-061; P. Xue was supported through the MIT Sea Grant 2006-RC-103; Z. Lai, J. Qi, and G. Cowles were supported through the Massachusetts Marine Fisheries Institute (NOAA grants NA04NMF4720332 and NA05NMF4721131); and R. Beardsley was supported through U.S. GLOBEC/Georges Bank NSF grant OCE-02227679, MIT Sea Grant NA06OAR1700019, and the WHOI Smith Chair in Coastal Oceanography

Pretreatment Donors after Circulatory Death with Simvastatin Alleviates Liver Ischemia Reperfusion Injury through a KLF2-Dependent Mechanism in Rat

Objective. Severe hepatic ischemia reperfusion injury (IRI) can result in poor short- and long-term graft outcome after transplantation. The way to improve the viability of livers from donors after circulatory death (DCD) is currently limited. The aim of the present study was to explore the protective effect of simvastatin on DCD livers and investigate the underlying mechanism. Methods. 24 male rats randomly received simvastatin or its vehicle. 30 min later, rat livers were exposed to warm ischemia in situ for 30 min. Livers were removed and cold-stored in UW solution for 24 h, subsequently reperfused for 60 min with an isolated perfused rat liver system. Liver injury was evaluated during and after warm reperfusion. Results. Pretreatment of DCD donors with simvastatin significantly decreased IRI liver enzyme release, increased bile output and ATP, and ameliorated hepatic pathological changes. Simvastatin maintained the expression of KLF2 and its protective target genes (eNOS, TM, and HO-1), reduced oxidative stress, inhibited innate immune responses and inflammation, and increased the expression of Bcl-2/Bax to suppress hepatocyte apoptosis compared to DCD control group. Conclusion. Pretreatment of DCD donors with simvastatin improves DCD livers’ functional recovery probably through a KLF2-dependent mechanism. These data suggest that simvastatin may provide a potential benefit for clinical DCD liver transplantation

MRI Findings for Frozen Shoulder Evaluation: Is the Thickness of the Coracohumeral Ligament a Valuable Diagnostic Tool?

Recent studies have demonstrated that the coracohumeral ligament (CHL) is shortened and thickened in a frozen shoulder. We analyzed the rate in CHL visualization between patients with frozen shoulder and normal volunteers using Magnetic Resonance Imaging (MRI) to determine the CHL thickness in the patients with a frozen shoulder.>0.05).MR Imaging is a satisfactory method for CHL depiction, and a thickened CHL is highly suggestive of frozen shoulder