Central Serous Chorioretinopathy after Solar Eclipse Viewing

Purpose: To report a case of central serous chorioretinopathy after solar eclipse viewing. Case Report: A middle-age man developed a sudden-onset unilateral scotoma after viewing a partial solar eclipse in Hong Kong. Fundus examination, fluorescein angiography, and optical coherence tomography showed features compatible with central serous chorioretinopathy. The patient was managed conservatively and reevaluated periodically. Serial optical coherence tomographic evaluations demonstrated an initial increase in the amount of subretinal fluid which spontaneously resolved 10 weeks after the onset of symptoms. Conclusion: This case demonstrates the possibility of development of central serous chorioretinopathy following solar eclipse viewing

The Spitzer c2d Survey of Nearby Dense Cores: III: Low Mass Star Formation in a Small Group, L1251B

We present a comprehensive study of a low-mass star-forming region,L1251B, at wavelengths from the near-infrared to the millimeter. L1251B, where only one protostar, IRAS 22376+7455, was known previously, is confirmed to be a small group of protostars based on observations with the Spitzer Space Telescope. The most luminous source of L1251B is located 5" north of the IRAS position. A near-infrared bipolar nebula, which is not associated with the brightest object and is located at the southeast corner of L1251B, has been detected in the IRAC bands. OVRO and SMA interferometric observations indicate that the brightest source and the bipolar nebula source in the IRAC bands are deeply embedded disk sources.Submillimeter continuum observations with single-dish telescopes and the SMA interferometric observations suggest two possible prestellar objects with very high column densities. Outside of the small group, many young stellar object candidates have been detected over a larger region of 12' x 12'. Extended emission to the east of L1251B has been detected at 850 micron; this "east core" may be a site for future star formation since no point source has been detected with IRAC or MIPS. This region is therefore a possible example of low-mass cluster formation, where a small group of pre- and protostellar objects (L1251B) is currently forming, alongside a large starless core (the east core).Comment: 35 pages, 15 figures, accepted for publication in ApJ, for the full resolution paper, visit "http://peggysue.as.utexas.edu/SIRTF/PAPERS/pap27.pub.pdf

DNA-damage induced cell death in yap1;wwtr1 mutant epidermal basal cells

In a previous study, it was reported that Yap1 and Wwtr1 in zebrafish regulates the morphogenesis of the posterior body and epidermal fin fold (Kimelman et al., 2017). We report here that DNA damage induces apoptosis of epidermal basal cells (EBCs) in zebrafish yap1-/-;wwtr1-/- embryos. Specifically, these mutant EBCs exhibit active Caspase-3, Caspase-8, and γH2AX, consistent with DNA damage serving as a stimulus of the extrinsic apoptotic pathway in epidermal cells. Live imaging of zebrafish epidermal cells reveals a steady growth of basal cell size in the developing embryo, but this growth is inhibited in mutant basal cells followed by apoptosis, leading to the hypothesis that factors underscoring cell size play a role in this DNA damage-induced apoptosis phenotype. We tested two of these factors using cell stretching and substrate stiffness assays, and found that HaCaT cells cultured on stiff substrates exhibit more numerous γH2AX foci compared to ones cultured on soft substrates. Thus, our experiments suggest that substrate rigidity may modulate genomic stress in epidermal cells, and that Yap1 and Wwtr1 promotes their survival

Review of Clinical Features, Microbiological Spectrum, and Treatment Outcomes of Endogenous Endophthalmitis over an 8-Year Period

Purpose. To evaluate the clinical features, microbiological spectrum, and treatment outcomes of endogenous endophthalmitis. Methods. Retrospective review of consecutive cases with infective endogenous endophthalmitis presenting from 2000 to 2007. The main outcome measure was the visual outcome at the latest follow-up visit. Other outcome measures included microbiological investigations, anatomical and clinical outcomes. Results. 22 eyes of 21 patients were included, and the mean follow-up duration was 2.7 years. Eyes with fungal endogenous endophthalmitis were more likely to have visual acuity of finger counting or better at presentation compared with those with bacterial endogenous endophthalmitis (odds ratio=15.0, =0.013). Gram-negative microorganisms accounted for 50% of infections, while fungal and gram-positive organisms accounted for 27.3% and 22.7%, respectively. Despite treatment, the visual outcome was poor in general as 10 (45.5%) eyes had no light perception at the latest follow-up visit and 6 (27.3%) eyes required enucleation or evisceration. Contrary to previous studies, fungal endogenous endophthalmitis did not appear to have better visual outcome compared with bacterial endogenous endophthalmitis. Conclusion. Gram-negative microorganisms were the main causative pathogens of endogenous endophthalmitis in Hong Kong. The visual prognosis of endogenous endophthalmitis is generally poor as almost 50% of eyes were blind despite treatment

Manganese Superoxide Dismutase and Chemokine Genes Polymorphisms in Chinese Patients with Anterior Uveitis

PURPOSE. To investigate the association of single-nucleotide polymorphisms (SNPs) in the manganese superoxide dismutase (MnSOD) and two chemokine genes (CCL2 and CCL5) in patients with anterior uveitis (AU). METHODS. Seventy-nine Chinese patients with acute AU were recruited, and genotyping of four SNPs including MnSOD 47, CCL2 Ϫ2518, CCL2 Ϫ2076, and CCL5 Ϫ403 alleles was performed with SNP genotyping assays. The genotype and allele frequencies were compared between patients with AU and 206 healthy control subjects. Analyses were also stratified according to the HLA-B27 status of the patients. RESULTS. There were significant increases in the frequency of the AA homozygosity in the MnSOD 47 SNP (P ϭ 0.049) and in the CCL2 Ϫ2518G allele frequency and GG homozygosity in patients with AU compared with control subjects (P ϭ 0.017 and P ϭ 0.024, respectively). No significant association was found between AU with the CCL2 Ϫ2076 and CCL5 Ϫ403 SNPs. Subgroup analyses showed that the MnSOD 47A polymorphism was significantly associated with AU in HLA-B27-positive patients, but not in HLA-B27-negative patients, whereas the CCL2 Ϫ2518G polymorphism was significantly associated with AU in HLA-B27-negative patients, but not in HLA-B27-positive patients. CONCLUSIONS. The 47A polymorphism in the MnSOD gene and the Ϫ2518G polymorphism in the CCL2 gene are associated with the development of AU in HLA-B27-positive and -negative Chinese patients, respectively. Further studies to evaluate the interactions of the HLA-B27 status and these SNPs are warranted. (Invest Ophthalmol Vis Sci

The Spitzer c2d Survey of Large, Nearby, Interstellar Clouds. VII. Ophiuchus Observed with MIPS

We present maps of 14.4 deg^2 of the Ophiuchus dark clouds observed by the Spitzer Space Telescope Multiband Imaging Photometer for Spitzer (MIPS). These high-quality maps depict both numerous point sources and extended dust emission within the star-forming and non–star-forming portions of these clouds. Using PSF-fitting photometry, we detect 5779 sources at 24 μm and 81 sources at 70 μm at the 10 σ level of significance. Three hundred twenty-three candidate young stellar objects (YSOs) were identified according to their positions on the MIPS/2MASS K versus color-magnitude diagrams, as compared to 24 μm detections in the SWIRE extragalactic survey. We find that more than half of the YSO candidates, and almost all those with protostellar Class I spectral energy distributions, are confined to the known cluster and aggregates

The SEGUE Stellar Parameter Pipeline. IV. Validation with an Extended Sample of Galactic Globular and Open Clusters

Spectroscopic and photometric data for likely member stars of five Galactic globular clusters (M3, M53, M71, M92, and NGC 5053) and three open clusters (M35, NGC 2158, and NGC 6791) are processed by the current version of the SEGUE Stellar Parameter Pipeline (SSPP), in order to determine estimates of metallicities and radial velocities for the clusters. These results are then compared to values from the literature. We find that the mean metallicity () and mean radial velocity () estimates for each cluster are almost all within 2{\sigma} of the adopted literature values; most are within 1{\sigma}. We also demonstrate that the new version of the SSPP achieves small, but noteworthy, improvements in estimates at the extrema of the cluster metallicity range, as compared to a previous version of the pipeline software. These results provide additional confidence in the application of the SSPP for studies of the abundances and kinematics of stellar populations in the Galaxy.Comment: 98 pages, 31 figures; accepted for publication in A

An IL1RL1 genetic variant lowers soluble ST2 levels and the risk effects of APOE-ε4 in female patients with Alzheimer’s disease

Changes in the levels of circulating proteins are associated with Alzheimer’s disease (AD), whereas their pathogenic roles in AD are unclear. Here, we identified soluble ST2 (sST2), a decoy receptor of interleukin-33–ST2 signaling, as a new disease-causing factor in AD. Increased circulating sST2 level is associated with more severe pathological changes in female individuals with AD. Genome-wide association analysis and CRISPR–Cas9 genome editing identified rs1921622, a genetic variant in an enhancer element of IL1RL1, which downregulates gene and protein levels of sST2. Mendelian randomization analysis using genetic variants, including rs1921622, demonstrated that decreased sST2 levels lower AD risk and related endophenotypes in females carrying the Apolipoprotein E (APOE)-ε4 genotype; the association is stronger in Chinese than in European-descent populations. Human and mouse transcriptome and immunohistochemical studies showed that rs1921622/sST2 regulates amyloid-beta (Aβ) pathology through the modulation of microglial activation and Aβ clearance. These findings demonstrate how sST2 level is modulated by a genetic variation and plays a disease-causing role in females with AD