Massive-Scale Agency

We are at a moment in time where technologies are developing at an accelerated pace, and information and communication technologies (ICTs) have advanced to a point where each of us carries a portal to insurmountable options for information access and social exchange. Our smartphones have become a necessary tool to the formation of our online and offline identities and will continue to be an access point to several emerging technologies that will further affect the way we inhabit our surrounding environment. With all the excitement that these technological advances may bring, we also find ourselves in a state of great uncertainty. The relationship we once had to the inner workings of our surrounding and ecological environments has deteriorated. This gap in knowledge and the resulting poor behaviours as it pertains to environmental sustainability have resulted in global warming, which continues to be the most pressing issue of our time. ICTs have increased the speed of communication to real-time, and this capability for near-instant feedback introduces the potential to re-establish a close relationship with our immediate environment. This thesis seeks to investigate how ICTs can be used to create a digital platform that facilitates new forms of information representation that bridge the gap between the individual and man-made climate change. It explores design solutions that can be produced when combining the architect’s skill set with tools and methodologies from other disciplines. Using various data collection methods including surveys, interviews, and user testing, a digital platform is created with the intention that its users may be able to gather their own evidence, realize where they are situated in the supply chain, and discover where there is room for individual agency through varying interventions

The Cognitive, Affective, and Somatic Empathy Scales (CASES): Cross-Cultural Replication and Specificity to Different Forms of Aggression and Victimization

A psychometrically sound measure of empathy that captures its multifaceted nature is critical in furthering research on empathy. The only instrument that assesses three domains of empathy together with positive and negative valence empathy is the newly developed 30-item cognitive, affective, and somatic empathy scales (CASES). The current study examines the cross-culture generalizability of CASES in Hong Kong and explores links between empathy and different forms of aggression and peer victimization. A sample of 4,676 Hong Kong youth (62% male) completed CASES, alongside measures of reactive/proactive aggression and multidimensional peer victimization. A subsample of youth (n = 2,321–2,464) and their parents completed additional instruments for testing the validity of CASES. We replicated most of the concurrent, convergent, and discriminant validity findings in the original development of CASES. Proactive aggression was most strongly linked to affective empathy, whereas reactive aggression was most strongly linked to somatic empathy. Differential associations were revealed between subscales of CASES and forms of peer victimization. Findings provide cross-cultural generalizability for a brief self-report instrument that captures the multifaceted nature of empathy. The multifaceted nature of empathy is further supported by differential associations with forms of aggression and victimization

Factors influencing dietary compliance amongst Australian diabetics

Managing the socio-economic burdens associated with the exponential global growth of diabetes diagnosis, poses one of the greatest challenges to modern health systems. Whilst there is no known cure for diabetes, many of the negative health impacts can be successfully minimised through formal therapy, dietary modification and exercise. In particular, dietary modification is considered an important first step and crucial for positive diabetes management and therapy outcomes. Despite this knowledge and extensive support and education provided by the health system, diabetes educators report that many people with diabetes are unable or unwilling to practice recommended dietary modification. This study empirically tests a model of factors that have been shown to play a role in influencing dietary compliance and confirms the importance of self-efficacy in this behaviour. The findings of this study are translated using a social marketing framework into recommendations for diabetes health educators

Limited genetic diversity in Salmonella enterica Serovar Enteritidis PT13

<p>Abstract</p> <p>Background</p> <p><it>Salmonella enterica </it>serovar Enteritidis has emerged as a significant foodborne pathogen throughout the world and is commonly characterized by phage typing. In Canada phage types (PT) 4, 8 and 13 predominate and in 2005 a large foodborne PT13 outbreak occurred in the province of Ontario. The ability to link strains during this outbreak was difficult due to the apparent clonality of PT13 isolates in Canada, as there was a single dominant pulsed-field gel electrophoresis (PFGE) profile amongst epidemiologically linked human and food isolates as well as concurrent sporadic strains. The aim of this study was to perform comparative genomic hybridization (CGH), DNA sequence-based typing (SBT) genomic analyses, plasmid analyses, and automated repetitive sequence-based PCR (rep-PCR) to identify epidemiologically significant traits capable of subtyping <it>S</it>. Enteritidis PT13.</p> <p>Results</p> <p>CGH using an oligonucleotide array based upon chromosomal coding sequences of <it>S. enterica </it>serovar Typhimurium strain LT2 and the <it>Salmonella </it>genomic island 1 successfully determined major genetic differences between <it>S</it>. Typhimurium and <it>S</it>. Enteritidis PT13, but no significant strain-to-strain differences were observed between <it>S</it>. Enteritidis PT13 isolates. Individual loci (<it>safA </it>and <it>fliC</it>) that were identified as potentially divergent in the CGH data set were sequenced in a panel of <it>S</it>. Enteritidis strains, and no differences were detected between the PT13 strains. Additional sequence-based typing was performed at the <it>fimA</it>, <it>mdh</it>, <it>manB</it>, <it>cyaA</it>, <it>citT</it>, <it>caiC</it>, <it>dmsA</it>, <it>ratA </it>and STM0660 loci. Similarly, no diversity was observed amongst PT13 strains. Variation in plasmid content between PT13 strains was observed, but macrorestriction with B<it>gl</it>II did not identify further differences. Automated rep-PCR patterns were variable between serovars, but <it>S</it>. Enteritidis PT13 strains could not be differentiated.</p> <p>Conclusion</p> <p>None of the methods identified any significant variation between PT13 strains. Greater than 11,300 base pairs of sequence for each of seven <it>S</it>. Enteritidis PT13 strains were analyzed without detecting a single polymorphic site, although diversity between different phage types of <it>S</it>. Enteritidis was observed. These data suggest that Canadian <it>S</it>. Enteritidis PT13 strains are highly related genetically.</p

LRH-1 drives colon cancer cell growth by repressing the expression of the <i>CDKN1A</i> gene in a p53-dependent manner

Liver receptor homologue 1 (LRH-1) is an orphan nuclear receptor that has been implicated in the progression of breast, pancreatic and colorectal cancer (CRC). To determine mechanisms underlying growth promotion by LRH-1 in CRC, we undertook global expression profiling following siRNA-mediated LRH-1 knockdown in HCT116 cells, which require LRH-1 for growth and in HT29 cells, in which LRH-1 does not regulate growth. Interestingly, expression of the cell cycle inhibitor p21 (CDKN1A) was regulated by LRH-1 in HCT116 cells. p21 regulation was not observed in HT29 cells, where p53 is mutated. p53 dependence for the regulation of p21 by LRH-1 was confirmed by p53 knockdown with siRNA, while LRH-1-regulation of p21 was not evident in HCT116 cells where p53 had been deleted. We demonstrate that LRH-1-mediated p21 regulation in HCT116 cells does not involve altered p53 protein or phosphorylation, and we show that LRH-1 inhibits p53 recruitment to the p21 promoter, likely through a mechanism involving chromatin remodelling. Our study suggests an important role for LRH-1 in the growth of CRC cells that retain wild-type p53

Public preferences for ecological indicators used in Everglades restoration

The Everglades is one of the largest wetland ecosystems in the world covering almost 18,000 square miles from central Florida southward to Florida Bay. Over the 20th century, efforts to drain the Everglades for agriculture and development severely damaged the ecosystem so that today roughly 50% of the historic flow of water through the Everglades has been diverted elsewhere. In an attempt to restore the Everglades, the U.S. Congress authorized the Comprehensive Everglades Restoration Plan (CERP) in 2000, expected to cost over $16 billion and to take several decades to complete. We used the results from a stated preference choice experiment (SPCE) survey of Florida households to estimate the willingness to pay for several ecological attributes related to CERP performance indicators likely to be impacted by Everglades restoration. We also used a latent class model (LCM) to explore preference heterogeneity among respondents. On average, survey respondents were willing to pay for improvements in all of the attributes included in the survey, namely increased populations of wading birds, American alligators, endangered snail kites, and spotted seatrout, and reduced polluted discharges from Lake Okeechobee to the Caloosahatchee and St. Lucie rivers. Willingness to pay was highest for reduced polluted discharges from Lake Okeechobee.Data Availability: All relevant data are available at https://doi.org/10.17605/OSF.IO/9AG7Z.S1 Table. Comparison between the demographics of the survey sample respondents and the Florida population based on 2010 U.S. Census information. https://doi.org/10.1371/journal.pone.0234051.s001S2 Table. Regression results from conditional logit model with willingness to pay estimates. https://doi.org/10.1371/journal.pone.0234051.s002The Everglades Foundationhttp://www.plosone.orghj2020Mammal Research Institut

Efficiently texturing hierarchical superhydrophobic fluoride-free translucent films by AACVD with excellent durability and self-cleaning ability

Translucent and superhydrophobic glass surfaces were fabricated by one-step deposition of a composite from the precursors, polydimethylsiloxane (PDMS) and tetraethyl orthosilicate (TEOS), via aerosol-assisted chemical vapour deposition. A raspberry-like hierarchical structure was obtained due to the nanoparticles being decomposed by the TEOS precursor and deposited around the micro-scale particles formed by the hydrolysis of the PDMS precursor. In this work, a translucent and superhydrophobic film was prepared by using optimized parameters (T: 290–330 °C, deposition time: 15–30 min) and the resulting water contact angle and sliding angle were >160° and <1°, respectively. It was found that there were 9 bounce cycles when water droplets were dropped onto such surfaces. Superior robustness was observed against tape-peeling, and on exposure to UV light (365 nm, 3.7 mW cm−2, 72 h) and to a large pH range (pH = 1–14, 72 h). The mechanical robustness was also examined and the results demonstrated that the film loses its superhydrophobicity when abraded for 5 meters with coarse sandpaper. The self-cleaning test demonstrated that the superhydrophobic surface could shed various contaminants and aqueous dyes, leaving a clear surface behind. This novel method can be applied to various substrates, including flexible (fabric and copper mesh) and rigid materials (copper block). This can provide a new, rapid and facile route for producing large-scale samples with multifunctional applications

Expression of CDK7, cyclin H and MAT1 is elevated in breast cancer and is prognostic in estrogen receptor- positive breast cancer

Purpose: CDK-activation kinase (CAK) is required for the regulation of the cell-cycle and is a trimeric complex consisting of Cyclin Dependent Kinase 7 (CDK7), Cyclin H and the accessory protein, MAT1. CDK7 also plays a critical role in regulating transcription, primarily by phosphorylating RNA polymerase II, as well as transcription factors such as estrogen receptor-α (ER). Deregulation of cell cycle and transcriptional control are general features of tumor cells, highlighting the potential for the use of CDK7 inhibitors as novel cancer therapeutics. Experimental Design: mRNA and protein expression of CDK7 and its essential co-factors cyclinH and MAT1, were evaluated in breast cancer samples to determine if their levels are altered in cancer. Immunohistochemical staining of >900 breast cancers was used to determine the association with clinicopathological features and patient outcome. Results: We show that expression of CDK7, cyclinH and MAT1 are all closely linked at the mRNA and protein level and their expression is elevated in breast cancer compared with the normal breast tissue. Intriguingly, CDK7 expression was inversely proportional to tumour grade and size and outcome analysis showed an association between CAK levels and better outcome. Moreover, CDK7 expression was positively associated with ER expression and in particular with phosphorylation of ER at serine 118, a site important for ER transcriptional activity. Conclusions: Expression of components of the CAK complex, CDK7, MAT1 and Cyclin H are elevated in breast cancer and correlates with ER. Like ERα , CDK7 expression is inversely proportional to poor prognostic factors and survival

Ceftiofur Resistance in Salmonella enterica Serovar Heidelberg from Chicken Meat and Humans, Canada

Use of this drug in chickens may limit effectiveness of cephalosporins in treating human infections