Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model

Extracellular vesicle (EV) secretion enables cell–cell communication in multicellular organisms. During development, EV secretion and the specific loading of signalling factors in EVs contributes to organ development and tissue differentiation. Here, we present an in vivo model to study EV secretion using the fat body and the haemolymph of the fruit fly, Drosophila melanogaster. The system makes use of tissue-specific EV labelling and is amenable to genetic modification by RNAi. This allows the unique combination of microscopic visualisation of EVs in different organs and quantitative biochemical purification to study how EVs are generated within the cells and which factors regulate their secretion in vivo. Characterisation of the system revealed that secretion of EVs from the fat body is mainly regulated by Rab11 and Rab35, highlighting the importance of recycling Rab GTPase family members for EV secretion. We furthermore discovered a so far unknown function of Rab14 along with the kinesin Klp98A in EV biogenesis and secretion

A marketing plan on JBL Home Theater Systems

The home theatre industry is a booming industry due to the advancement in technology, not just in audio equipment but in all media players. Reports show that annual gross income and disposable income have been increasing for the previous years. The growth is partially due to the large amount of OFW remittances that are coming in the country. Number of households is seen to be growing as well. This implies that more people can now afford to construct homes or renovate their own homes to make it bigger and better. Aside from that, Filipinos now have more disposable income that they can use for leisure and recreational products such as home theatre systems. The JBL Studio L Series is engineered to make the same clear, accurate and dynamic sound quality heard by the studio pros available to home theatre enthusiasts and music lovers alike. Consumers not only get high quality sounds in their homes but the prestige that comes with owning such a luxurious product. The target market of the product are males ranging from 30 60 and from socio-economic classes A and B since they are the ones who can afford such high end products. To reach and penetrate the company\u27s target market, the product will be advertised through flyers, posters, lifestyle magazines, and newspapers. Non-traditional advertisements through online websites such as Facebook will also be used to increase awareness. To encourage repeat purchases and improve customer relations and brand loyalty, the company will make use of sales promos as well as public relations advertising like tie up events and after sales letters