DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR ESTIMATION OF BRIMONIDINE TARTRATE AS BULK DRUG AND IN OPHTHALMIC FORMULATION

The optimized reverse phase high performance liquid chromatographic method was developed for estimation of Brimonidine Tartrate in bulk drug and pharmaceutical dosage form. Chromatography was performed on Kromasil C 18 ( 250 mm X 4.6 mm i.d. , 5 μm particle size) column with mobile phase citric acid monohydrate buffer:water:methanol (30:50:20 v/v/v) and  pH 3 was maintained by using triethylamine. The flow rate was 1.0 ml/min. Elute was detected at 246 nm and it effectively separated at Retention Time of 5.96 min. The LOD and LOQ was 1.47 and 4.47 μg/ml respectively. A linear response was observed over the concentration range 40-80 μg/ml for Brimonidine Tartrate. Thus the proposed HPLC method was found accurate, specific, precise, robust and reproducible

Polymer quantization of CGHS model- I

We present a polymer(loop) quantization of a two dimensional theory of dilatonic gravity known as the CGHS model. We recast the theory as a parametrized free field theory on a flat 2-dimensional spacetime and quantize the resulting phase space using techniques of loop quantization. The resulting (kinematical) Hilbert space admits a unitary representation of the spacetime diffeomorphism group. We obtain the complete spectrum of the theory using a technique known as group averaging and perform quantization of Dirac observables on the resulting Hilbert space. We argue that the algebra of Dirac observables gets deformed in the quantum theory. Combining the ideas from parametrized field theory with certain relational observables, evolution is defined in the quantum theory in the Heisenberg picture. Finally the dilaton field is quantized on the physical Hilbert space which carries information about quantum geometry.Comment: 50 pages, 2 figure

LHC Magnet Tests: Operational Techniques and Empowerment for Successful Completion

The LHC magnet tests operation team developed various innovative techniques, particularly since early 2004, to complete the superconductor magnet tests by Feb. 2007. Overall and cryogenic priority handling, rapid on-bench thermal cycling, rule-based goodness evaluation on round-the-clock basis, multiple, mashed web systems are some of these techniques applied with rigour for successful tests completion in time. This paper highlights these operation empowerment tools which had a pivotal role for success. A priority handling method was put in place to enable maximum throughput from twelve test benches, having many different constraints. For the cryogenics infrastructure, it implied judicious allocation of limited resources to the benches. Rapid On-Bench Thermal Cycle was a key strategy to accelerate magnets tests throughput, saving time and simplifying logistics. First level magnet appraisal was developed for 24 hr decision making so as to prepare a magnet further for LHC or keep it on standby. Web based systems (Tests Management and E-Traveller) were other essential ideas to track & coordinate various stages of tests handled by different teams