Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis

The aim of this study was to investigate isolates of Actinobacillus pleuropneumoniae previously designated serologically either as NT or as ‘K2:07’, which did not produce serovar-specific amplicons in PCR assays. We used whole genome sequencing to identify the capsule (CPS) loci of six previously designated biovar 1 non-typable (NT) and two biovar 1 ‘K2:O7’ isolates of A. pleuropneumoniae from Denmark, as well as a recent biovar 2 NT isolate from Canada. All of the NT isolates have the same six-gene type I CPS locus, sharing common cpsABC genes with serovars 2, 3, 6, 7, 8, 9, 11 and 13. The two ‘K2:O7’ isolates contain a unique three-gene type II CPS locus, having a cpsA gene similar to that of serovars 1, 4, 12, 14 and 15. The previously NT isolates share the same O-antigen genes, found between erpA and rpsU, as serovars 3, 6, 8, and 15. Whereas the ‘K2:O7’ isolates, have the same O-antigen genes as serovar 7, which likely contributed to their previous mis-identification. All of the NT and ‘K2:O7’ isolates have only the genes required for production of ApxII (apxIICA structural genes, and apxIBD export genes). Rabbit polyclonal antisera raised against representative isolates with these new CPS loci demonstrated distinct reactivity compared to the 16 known serovars. The serological and genomic results indicate that the isolates constitute new serovars 17 (previously NT) and 18 (previously ‘K2:O7’). Primers designed for amplification of specific serovar 17 and 18 sequences for molecular diagnostics will facilitate epidemiological tracking of these two new serovars of A. pleuropneumoniae

Liberalization, Contention, and Threat: Institutional Determinates of Societal Preferences and the Arab Spring in Tunisia and Morocco

Why do revolutions happen? What role do structures, institutions, and actors play in precipitating (or preventing) them? Finally, What might compel social mobilization against a regime in the face of potentially insurmountable odds? These questions are all fundamentally about state-society (strategic) interactions, and elite and societal preference formation over time. The self-immolation of Muhammad Bouazizi in Sidi Bouzid on December 17, 2010, served as a focal point upon which over twenty years of corrupt, coercive authoritarian rule were focused into a single, unified challenge to the Ben Ali regime. The regime\u27s brutality was publicized via social media activism and satellite television, precipitating mass mobilization across Tunisia and, eventually, throughout the region and beyond. In light of the rapid and unforeseen nature of these events, scholars writing about the causes of the Arab Spring have focused their critiques on scholarship that they felt overemphasized the role of institutions and elite-level actors over \u27under the radar\u27 changes within society. This paper essentially agrees with this point of view, but is not content to simply \u27throw out\u27 institutionalism. As Timur Kuran (1991) argued in the wake of the unforeseen collapse of communism in Eastern Europe, one cannot understand revolution without understanding the \u27true\u27 preferences of social actors. In this way, the inevitability of revolutionary surprises seems a given so long as analysts continue to look from the top-down. Yet, this paper contends that institutions do still matter. They matter because different institutional arrangements incentivize and constrain regime strategies, which, in turn, inform the strategic calculations and preference orderings within society. These two societal variables are determined - in part - by the degree of regime flexibility, and they affect whether, how, and where social actors choose to vent their dissent. This paper proposes a model for the development of contentious social mobilization under authoritarianism. In order to do so, two models - one game-theoretic, and the other rooted in the contentious politics subfield of political sociology - are synthesized toward elucidating how altered societal preferences affect strategic interactions between the regime and society over time and during acute contentious episodes. The synthesized model is then illustrated through narrative case studies of two North African states that experienced divergent outcomes in the wake of the Arab Spring: Tunisia and Morocco. The limited spaces and institutions for the expression of dissent in Tunisia gradually changed societal preferences over time. In 2010, Tunisians\u27 preferences shifted from various socioeconomic demands and other issue-specific grievances toward a galvanized demand for the fall of the regime. In Morocco, on the other hand, social actors, by and large, continued to prefer limited reforms to a complete upheaval of the political system. This paper contends that this divergence in preferences and therefore outcomes was in part determined by the variation in the two regimes\u27 respective strategic mixes of concessions and/or coercion. To the extent that such strategies and institutions were more flexible - i.e. were more permissive of (limited) political contention and contestation - social movements were less likely to become emboldened against the regime

Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405 : comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, BB/G018553/1, BB/S002103/1, and BB/S005897/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. Funding for LL provided by the ‘National Natural Science Foundation of China’ (No.31520103917). MTGH and DH were supported by the Wellcome Trust (grant number 098051).We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.Publisher PDFPeer reviewe