Phenotype and envelope gene diversity of -deleted HIV-1 isolated from long-term survivors infected from a single source-0

<p><b>Copyright information:</b></p><p>Taken from "Phenotype and envelope gene diversity of -deleted HIV-1 isolated from long-term survivors infected from a single source"</p><p>http://www.virologyj.com/content/4/1/75</p><p>Virology Journal 2007;4():75-75.</p><p>Published online 16 Jul 2007</p><p>PMCID:PMC1939844.</p><p></p>roduction in culture supernatants was measured by RT assays. Values shown are means from duplicate infections. Error bars represent standard deviations. Results are representative of two independent experiments using cells obtained from different donors

Phenotype and envelope gene diversity of -deleted HIV-1 isolated from long-term survivors infected from a single source-4

<p><b>Copyright information:</b></p><p>Taken from "Phenotype and envelope gene diversity of -deleted HIV-1 isolated from long-term survivors infected from a single source"</p><p>http://www.virologyj.com/content/4/1/75</p><p>Virology Journal 2007;4():75-75.</p><p>Published online 16 Jul 2007</p><p>PMCID:PMC1939844.</p><p></p>-1 infected PBMC and subjected to GeneScan analysis, as described in the Methods and elsewhere [41, 56]. (A) GeneScan sample files generated from amplified products. (B) Fraction of sequences with a given V1V2 nucleotide length, which was calculated from GeneScan sample files. Peaks and bars shown in red represent V1V2 amplimers from early viruses, and peaks and bars shown in blue represent V1V2 amplimers from late viruses. Similar results were obtained in two independent experiments

MOESM4 of HIV integration and the establishment of latency in CCL19-treated resting CD4+ T cells require activation of NF-ÎşB

Additional file 4: Figure S4. Integration site selection and gene activation in chemokine treated cells. A, Gene expression was determined by Illumina bead array in unactivated, CCL19-treated or PHA-IL2 activated CD4+ T cells after 6 or 72 h. The ratio of expression of genes at the sites of integration was determined in each in vitro condition. B, Expression of individual genes at the site of HIV integration in CCL19-treated resting CD4+ T cells (x-axis) compared to unactivated (y-axis; upper panel) or PHA-IL2 activated CD4+ T cells (y-axis; lower panel). C, The distance of integration sites to specific genomic elements including LINE, H4K20me3 and H4R3me following HIV infection of unactivated, CCL19-treated and PHA-IL2 activated CD4+ T cells, or CD4+ T cells from HIV-infected patients on cART or randomly selected sites. Log distance is shown as box plots (median and quartiles) with violin plot of the kernel distribution. The means are shown as a red horizontal line