Identification of B- and T-Cell Epitopes of BB, a Carrier Protein Derived from the G Protein of Streptococcus Strain G148

Most conventional vaccines consist of killed organisms or purified antigenic proteins. Such molecules are generally poorly immunogenic and need to be coupled to carrier proteins. We have identified a new carrier molecule, BB, derived from the G protein of Streptococcus strain G148. We show that BB is able to induce strong antibody responses when conjugated to peptides or polysaccharides. In order to localize T and B cell epitopes in BB and match them with the albumin-binding region of the molecule, we immunized mice with BB, performed B and T pepscan analyses, and compared the results with pepscan done with sera and cells from humans. Our results indicate that BB has two distinct T helper epitopes, seven linear B-cell epitopes, and one conformational B-cell epitope in BALB/c mice. Four linear B-cell epitopes were identified from human sera, three of which overlapped mouse B-cell epitopes. Finally, three human T-cell epitopes were detected on the BB protein. One of these T-cell epitopes is common to BALB/c mice and humans and was localized in the region that contains the albumin-binding site. These data are of interest for the optimization of new carrier molecules derived from BB

Absolute and multiplex quantification of antibodies in serum using PSAQ™ standards and LC-MS/MS

International audienc

Vienne-Prague

Ce numéro double propose une histoire culturelle des relations austro-tchécoslovaques au XXe siècle, à la lumière de figures d’intellectuels et de médiateurs culturels ayant favorisé les transferts culturels entre Vienne et Prague, notamment Thomas Masaryk, les écrivains du Cercle de Prague, Leoš Janáček, Adolf Loos, Richard Teschner, Jiří Gruša, ainsi que trois revues : Musikblätter des Anbruch, Prager Presse et Wir und Sie im Herzen Europas