Enteric viruses as contaminants and bioindicators in environmental samples

Submitted by Luciana Ferreira ([email protected]) on 2018-08-28T12:21:29Z No. of bitstreams: 2 Artigo - Gislaine Fongaro - 2015.pdf: 280159 bytes, checksum: ef6ff60369c5c487e0dfedbdd6be497d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2018-08-28T12:24:51Z (GMT) No. of bitstreams: 2 Artigo - Gislaine Fongaro - 2015.pdf: 280159 bytes, checksum: ef6ff60369c5c487e0dfedbdd6be497d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-08-28T12:24:51Z (GMT). No. of bitstreams: 2 Artigo - Gislaine Fongaro - 2015.pdf: 280159 bytes, checksum: ef6ff60369c5c487e0dfedbdd6be497d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-12Gastroenteritis, diarrhea, and other diseases can be caused by enteric viruses transmitted by fecal-oral route. Human adenoviruses (HAdV), rotavirus A and C (RVA and RVC, respectively), hepatitis A and E virus (HAV and HEV, respectively), human astroviruses (HAstV), human noroviruses (HuNV) and enteroviruses (EV) are, among the enteric viruses, the most frequently detected in environment samples. These viruses are usually introduced into aquatic environments by human, industrial, or agricultural activities and are widely distributed all over the world. They have the common characteristics to be structurally stable and can also absorb to solid particles and biofilms, thereby protecting themselves from inactivating factors. This revision aimed to present and discuss: i) most relevant enteric viruses for human and animal health; ii) enteric viruses as contaminants and bioindicators in environmental samples; iii) molecular and cell culture methods for enteric virus detection; iv) use of enteric viruses for microbial risk assessment. Impacts of enteric viruses on environment and the potential use as bioindicators of the sanitary security, such as presence and infectivity studies were discussed as development of new tools for disinfection, monitoring, risk modeling and management, among other studies

Cytotoxic activity and structural features of Ru(II)/phosphine/amino acid complexes.

Thirteen new ruthenium amino acid complexes were synthesized and characterized. They were obtained by the reaction of ?-amino acids (AA) with [RuCl2(P-P)(N-N)], where P-P = 1,4-bis(diphenylphosphino)butane (dppb) or 1,3-bis(diphenylphosphino)propane (dppp) and N-N = 4,4?-dimethyl-2,2?-bipyridine (4?-Mebipy), 5,5?-dimethyl-2,2?-bipyridine (5?-Mebipy) or 4,4?-Methoxy-2-2?-bipyridine (4?-MeObipy). This afforded a family of complexes formulated as [Ru(AA-H)(P-P)(N-N)]PF6, where AA = glycine (Gly), L-alanine (Ala), L-valine (Val), L-tyrosine (Tyr), L-tryptophan (Trp), L-histidine (His) and L-methionine (Met). All compounds were characterized by elemental analysis, spectroscopic and electrochemical techniques. The [Ru(AA-H)(P-P)(N-N)]PF6 complexes are octahedral (the AA-H ligand binding involves N-amine and O-carboxylate), diamagnetic (low-spin d6, S = 0) and present bands due to electronic transitions in the visible region. 1H, 13C{1H} and 31P{1H} NMR spectra of the complexes indicate the presence of C2 symmetry, and the identification of diastereoisomers. In vitro cytotoxicity assays of the compounds and cisplatin were carried out using MDA-MB-231 (human breast) tumor cell line and a non-tumor breast cell line (MCF-10A). Most complexes present promising results with IC50 values comparable with the reference drug cisplatin and high selectivity indexes were found for the complexes containing L-Trp. The binding of two Ru-precursors of the type [RuCl2(dppb)(NN)] (N-N = 4?-MeObipy or 4?-Mebipy) to the blood transporter protein human serum albumin (HSA) was evaluated by fluorescence and circular dichroism spectroscopy. Both complexes bind HSA, probably in the hydrophobic pocket near Trp214, and the Ru-complex containing 4?-MeObipy shows higher affinity for HSA than the 4?-Mebipy one