Effect of different physical treatments on antioxidant activity of jenny milk

The shelf life of food can be extended by employing several physical treatments. Among them, pasteurization and condensation are widely adopted for prolonging the shelf-life of milk by reducing the microbial load. However, these treatmens could affect the antioxidant activity of the product. The aim of the present study was to investigate the effect of pasteurization and condensation on the Total Antioxidant Capacity (TAC) of raw and pasteurised jenny milk. Using 2,2’-azinobis (3-ethylbenzthiazoline-6-acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. TAC was also measured after condensation at 40 and at 20% of the initial volume. ABTS assay was found to be more accurate and reproducible than DPPH. Pasteurisation reduced significantly (P<0.05) the total antioxidant capacity from 84.15to 82.09% (ABTS test). The TAC decreased with the increase of condensation in raw and pasteurised milk. Overall, the TAC was reduced by only 3 and 4%, in pasteurised and in raw milk, respectively (ABTS test). Therefore, both pasteurization and condensation are recommended as physical treatments to prolong the shelf-life of jenny milk

Fatality rate and predictors of mortality in an Italian cohort of hospitalized COVID-19 patients

Clinical features and natural history of coronavirus disease 2019 (COVID-19) differ widely among different countries and during different phases of the pandemia. Here, we aimed to evaluate the case fatality rate (CFR) and to identify predictors of mortality in a cohort of COVID-19 patients admitted to three hospitals of Northern Italy between March 1 and April 28, 2020. All these patients had a confirmed diagnosis of SARS-CoV-2 infection by molecular methods. During the study period 504/1697 patients died; thus, overall CFR was 29.7%. We looked for predictors of mortality in a subgroup of 486 patients (239 males, 59%; median age 71 years) for whom sufficient clinical data were available at data cut-off. Among the demographic and clinical variables considered, age, a diagnosis of cancer, obesity and current smoking independently predicted mortality. When laboratory data were added to the model in a further subgroup of patients, age, the diagnosis of cancer, and the baseline PaO2/FiO2 ratio were identified as independent predictors of mortality. In conclusion, the CFR of hospitalized patients in Northern Italy during the ascending phase of the COVID-19 pandemic approached 30%. The identification of mortality predictors might contribute to better stratification of individual patient risk

Germination Behavior and Geographical Information System-Based Phenotyping of Root Hairs to Evaluate the Effects of Different Sources of Black Soldier Fly (Hermetia illucens) Larval Frass on Herbaceous Crops

: Insect larval frass has been proposed as a fertilizer and amendment, but methods for testing its effects on plants are poorly developed and need standardization. We obtained different types of black soldier fly (Hermetia illucens) frass via the factorial combination of (a) two insect diets, as follows: G (Gainesville = 50% wheat bran, 30% alfalfa meal, 20% maize meal) and W (43% sheep whey + 57% seeds); (b) two frass thermal treatments: NT = untreated and T = treated at 70 °C for 1 h. We tested the effects on the germination of cress (Lepidium sativum L.) and wheat (Triticum durum Desf.) by applying 1:2 w:w water extracts at 0, 25, 50, 75 and 100% concentration. Standardizing frass water content before extraction affected chemical composition. Frass extracts showed high electrical conductivity (8.88 to 13.78 mS cm-1). The W diet was suppressive towards Escherichia coli and showed a lower content of nitrates (e.g., WNT 40% lower than GNT) and a concentration-dependent phytotoxic effect on germinating plants. At 25% concentration, germination indices of G were 4.5 to 40-fold those at 100%. Root and shoot length and root hair area were affected by diet and concentration of frass extracts (e.g., root and shoot length in cress at 25% were, respectively, 4.53 and 2 times higher than at 100%), whereas the effects of the thermal treatment were few or inconclusive. On barley (Hordeum vulgare L.) grown in micropots on a silty loam soil, root mass was reduced by 37% at high extract concentration. A quick procedure for root hair surface area was developed based on the geographic information system (GIS) and may provide a fast method for incorporating root hair phenotyping in frass evaluation. The results indicate that below-ground structures need to be addressed in research on frass effects. For this, phyotoxicity tests should encompass different extract dilutions, and frass water content should be standardized before extraction in the direction of canonical procedures to allow comparisons

Soil structure and stability in the spermosphere of myxosdiaspore chia (Salvia hispanica L.)

Mucilage produced by myxodiaspores has been mainly studied for its role in seed ecology. This work investigates changes due to mucilage in soil structure and stability in the spermosphere of the myxodiaspore crop chia (Salvia hispanica L.). In sandy-loam (S) repacked soil sown with chia and subjected to wetting–drying (DW), Computer Assisted X-ray micro tomography showed that extrusion of a mucilage capsule formed a pore of volume twice that of the seed, surrounded by a shell of particles with higher porosity and lower pore size than the bulk soil. In three soils (S; loam, L; and clay-loam, C) the aggregate stability index to wet sieving (Stw) decreased with one and two DW cycles. Application of 2% w/w mucilage increased Stw before and after DW, from 29% in C to more than 60-fold in S. Mucilage-amended samples after DW had higher or equal Stw compared with unamended soil before DW. Soil retained at the surface of hydrated diaspores exposed to water flow changed with soil texture (11.3, 90.5 and 91.7 mg on each hydrated seed for S, L and C respectively) and chia genotype (6.7, 9.9 and 12.8 mg per hydrated seed in BC and G8 long-day flowering mutants and commercial Black chia respectively). Our results showed that myxodiaspores affected soil structure by providing a microenvironment of altered porosity and high stability around the mucilage capsule extruded by the hydrated seed. This finding characterises a transient but crucial time of crop production around sowing when physical properties of soil surrounding seeds are relevant to stability, germination and plant–microorganism relations

Fodder Yield, Quality and Growth of Chia (Salvia hispanica L.) as Affected by Sowing Density and Top-Dressing Nitrogen Fertilization

Chia (Salvia hispanica L.) seeds are considered a superfood, but research on vegetative biomass uses is scarce. We conducted a two-year trial under the hypothesis that short-day flowering black chia would provide quality forage at high latitudes and tested sowing density (D1 = 125, D2 = 25, D3 = 8 and D4 = 4 plants m(-2)) and top-dressing N fertilization (N = 0, N = 20 kg ha(-1)) at three stages (EV = early vegetative, LV = late vegetative and EF = early flowering). Forage yield reached 9.0 and 5.64 t ha(-1) of total and 2.07 and 1.56 t ha(-1) of leaf dry mass at EF in 2013 and 2014, respectively. High plant density corresponded to higher biomass but to lower plant relative growth rate, stem diameter, branching, proportion of leaves and lipid and crude protein content. Crude protein declined from 18% at EV to about 8% at EF and was lower in D1 than in D4. Lipids ranged between 33.83 g kg(-1) (D4, EV) and 17.34 g kg(-1) (D1, EF) (p < 0.005). N topdressing affected forage quality but not yield. Alpha-linolenic acid was the most abundant fatty acid (FA) (608 g kg(-1) FA at EV). The long vegetative growth of short-day flowering chia in southern Europe is favorable to fodder production, and management should be optimized by seeking balance between growth rate and stand density while optimizing vegetative stage growth for the highest forage quality

Effects of Soil Water Shortage on Seedling Shoot and Root Growth of Saragolle Lucana Tetraploid Wheat (Triticum durum Desf.) Landrace

Ancient wheats may be a source of traits that are useful for the tolerance of climate change foreseen conditions of raising temperatures and low water availability. Previous research has shown a fine root system and a high mass of rhizosheath per unit root mass in the italian durum wheat (Triticum durum Desf) landrace Saragolle Lucana, and this may be relevant for successfully facing adverse conditions during seedling establishment. We investigated the effect of soil water shortage in Saragolle seedlings on root architecture, rhizosheath formation and biomass allocation. Pot experiments were conducted by comparing two levels of soil available water content (AWC): WW (100% of AWC) and DS (50% of AWC). Phenology was delayed by eight days in DS and above and belowground traits were measured at Zadoks 1.3 for each treatment. Biometric data collected at the same phenological stage show that DS plants did not reach the levels of biomass, surface area and space occupation of WW even after attaining the same developmental stage. Namely, plant dimensions were lower at low soil water availability, with the exception of rhizosheath production: DS yielded a 50% increase in rhizosheath mass and 32% increase in rhizosheath mass per unit root mass. The proportion of plant mass reduction in DS was 29.7% for aboveground parts and 34.7% for roots, while reductions in leaf and root surface areas exceeded 43%. The root/shoot mass and area ratios were not significantly different between treatments, and a higher impact on aboveground than on belowground traits at reduced available water was shown only by a lower ratio of shoot height to root depth in DS than in WW. Increases in rhizosheath in absolute and relative terms, which were observed in our experiment in spite of smaller root systems in the ancient durum wheat variety Saragolle lucana at DS, may provide an interesting trait for plant performance in conditions of low soil water availability both for water-related issue and for other effects on plant nutrition and relations with the rhizosphere

Effect of sowing density and nitrogen top-dress fertilisation on growth and yield of chia (Salvia hispanica L.) in a Mediterranean environment: first results

The demand for sources of nutraceuticals has led to the rediscovery and diffusion of traditional crops such as chia (Salvia hispanica L.), whose leaves and fruits are rich in Ω3 fatty acids and anti-oxidants. Chia originates in Central America but it is rapidly expanding to new areas. A field experiment conducted at Atella in Basilicata (Southern Italy) was set up to test the response of chia to N top-dress fertilisation (0 and 20 kg ha-1) and to sowing density (D1=125, D2=25, D3=8 and D4=4 plants m-2) in a split-plot design with three replications. First results show maximum leaf area index values up to 7.1 and fresh vegetative biomass production at early flowering ranging between 50.87 (D4) and 59.71 (D1) t ha-1. Yield increased with plant density: a significantly (P<0.01) higher production (398 kg ha–1) was reached in D1. N top-dressing had a detrimental effect on yield and corresponded to higher lodging and lower maturation percentage of seeds, though non-significant. Based on our first results it seems worthwhile to continue agronomical trials for chia in herbaceous systems of southern Italy for leaf production based on traditional genotypes, while fruit production might be pursued by adopting high sowing density and the search for longer-day genotypes

Root Morphology, Allometric Relations and Rhizosheath of Ancient and Modern Tetraploid Wheats (Triticum durum Desf.) in Response to Inoculation with Trichoderma harzianum T-22

Early root traits and allometrics of wheat are important for competition and use of resources. They are under-utilized in research and un-explored in many ancient wheats. This is especially true for the rhizosheath emerging from root-soil interactions. We investigated root morphology, root/shoot relations and the amount of rhizosheath of four tetrapoid wheat seedlings (30 days after emergence): the italian landrace Saragolle Lucana and modern varieties Creso, Simeto and Ciclope, and tested the hypothesis that inoculation with Trichoderma harzianum T-22 (T-22) enhances rhizosheath formation and affects wheat varieties differently. Overall growth of non-inoculated plants showed different patterns in wheat varieties, with Saragolle and Ciclope at the two extremes: Saragolle invests in shoot rather than root mass, and in the occupation of space with highest (p &lt; 0.05) shoot height to the uppermost internode (5.02 cm) and length-to-mass shoot (97.8 cm g&minus;1) and root (more than 140 m g&minus;1) ratios. This may be interpreted as maximizing competition for light but also as a compensation for low shoot efficiency due to the lowest (p &lt; 0.05) recorded values of optically-measured chlorophyll content index (22.8). Ciclope invests in biomass with highest shoot (0.06 g) and root (0.04 g) mass and a thicker root system (average diameter 0.34 mm vs. 0.29 in Saragolle) as well as a highest root/shoot ratio (0.95 g g&minus;1 vs. 0.54 in Saragolle). Rhizosheath mass ranged between 22.14 times that of shoot mass in Ciclope and 43.40 in Saragolle (different for p &lt; 0.05). Inoculation with Trichoderma increased the amount of rhizosheath from 9.4% in Ciclope to 36.1% in Simeto and modified root architecture in this variety more than in others. Ours are the first data on roots and seedling shoot traits of Saragolle Lucana and of Trichoderma inoculation effects on rhizosheath. This opens to new unreported interpretations of effects of Trichoderma inoculation on improving plant growth