Management and Care of Women With Invasive Cervical Cancer: American Society of Clinical Oncology Resource-Stratified Clinical Practice Guideline

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Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved]

Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription

Preservation of Strawberries with an Antifungal Edible Coating Using Peony Extracts in Chitosan

Strawberries represent a valuable source of bioactive compounds including vitamin C, E, β-carotene, and phenolic compounds, but they are fruits with an extremely short postharvest life. Therefore, the current study was conducted to investigate the effectiveness of an active coating consisting in antifungal microparticles obtained by spray drying of peony extracts (Paeonia rockii (PPR)) dispersed in chitosan (Ch) and subsequent addition to polysaccharide gels to slow the fungal attack of small highly perishable fruits, such as strawberries. The results of the antimicrobial assays indicate that the peony extracts in chitosan are able to counteract effectively the growth of different fungal isolates from deteriorated strawberries. In conclusion, through the treatment with this antifungal coating, it is possible to prolong the shelf life of delicate fruits, such as strawberries, to about 16Ã\u82 days, slowing down the weight loss, affecting the safeguard of important vitamins and antioxidant capacity during storage, without causing any significant alteration of the nutritional and sensorial properties of the product