TCD4+ Lymphocyte are Related to Muscle Strength Parameters in HIV-1 Positive Adolescents: A Preliminary Study

Introduction: Catabolism of muscle and loss of function are complications that can occur during the course of HIV infection, and are commonly seen in a majority of adolescents with vertically transmitted Human Immunodeficiency Virus-type 1 (HIV-1). The levels of CD4+ and CD8+ lymphocytes, reference markers for the treatment of vertically transmitted HIV-1, also decline as the disease progresses. Alterations on these reference markers may be associated with neuromuscular force parameters in sedentary adolescents that have potential as prognostic indicators for treatment administration. Objective: To investigate the relationship between maximal isometric muscular contraction force and levels of CD4+ and CD8+ lymphocytes in sedentary, vertically transmitted HIV-1 positive adolescents. Methods: The sample consisted of twenty individuals, adolescents (N= 9 males, 11 females, age 15-17 years), vertically transmitted HIV-1 patients from Institute of Infectious Diseases Emilio Ribas in São Paulo, Brazil, who were undergoing HAART therapy randomly selected to participate in the study. The number of CD4+ and CD8+ cells was determined by flow cytometry using BD FacsCalibur Multitest Equipment, and Multiset-BD software. Viral load was determined using b-DNA methodology, on Siemens System Versátil 440 equipment. All analyses followed standard procedures approved by the Brazilian Ministry of Health. Muscular strength measurements were completed in the morning after blood collection and weight and height measurements. Prior to starting, patients were familiarized with all testing procedures and strength exercises that were used for testing. After the warm up, maximum voluntary isometric muscular strength of the elbow flexors and knee extensors were assessed using an electric dynamometer (EMG210C, EMGLAB System of Brazil). Each patient made three attempts with a rest interval of two minutes between trials. The highest isometric force and torque value were recorded and used for analyses. Results: Upper body force (r=0.70, p=0.001) and maximal torque (r=0.69, p=0.001) were significant correlated with CD4+ count. Similar observations between CD4+ count and lower body muscular force (r=0.62, p=0.005) and maximum torque (r=0.61, p=0.007) were also observed. CD8+ was not associated with any strength measures. Conclusion: CD4+ lymphocytes showed a strong correlation with force parameters in sedentary HIV-1 positive adolescents. Given that those individuals with higher TCD4+ counts showed a tendency towards manifesting higher muscle strength, this may be used as a predictor to indicate the level of physical capacity of patients and consequently help optimize treatment. Further research is needed to explore the potential prognostic value of muscle strength parameters in HIV-1 positive adolescents

The effects of salbutamol on epithelial ion channels depend on the etiology of acute respiratory distress syndrome but not the route of administration.

Abstract INTRODUCTION: We investigated the effects of intravenous and intratracheal administration of salbutamol on lung morphology and function, expression of ion channels, aquaporin, and markers of inflammation, apoptosis, and alveolar epithelial/endothelial cell damage in experimental pulmonary (p) and extrapulmonary (exp) mild acute respiratory distress syndrome (ARDS). METHODS: In this prospective randomized controlled experimental study, 56 male Wistar rats were randomly assigned to mild ARDS induced by either intratracheal (n = 28, ARDSp) or intraperitoneal (n = 28, ARDSexp) administration of E. coli lipopolysaccharide. Four animals with no lung injury served as controls (NI). After 24 hours, animals were anesthetized, mechanically ventilated in pressure-controlled mode with low tidal volume (6 mL/kg), and randomly assigned to receive salbutamol (SALB) or saline 0.9% (CTRL), intravenously (i.v., 10 mug/kg/h) or intratracheally (bolus, 25 mug). Salbutamol doses were targeted at an increase of [almost equal to] 20% in heart rate. Hemodynamics, lung mechanics, and arterial blood gases were measured before and after (at 30 and 60 min) salbutamol administration. At the end of the experiment, lungs were extracted for analysis of lung histology and molecular biology analysis. Values are expressed as mean +/- standard deviation, and fold changes relative to NI, CTRL vs. SALB. RESULTS: The gene expression of ion channels and aquaporin was increased in mild ARDSp, but not ARDSexp. In ARDSp, intravenous salbutamol resulted in higher gene expression of alveolar epithelial sodium channel (0.20 +/- 0.07 vs. 0.68 +/- 0.24, p < 0.001), aquaporin-1 (0.44 +/- 0.09 vs. 0.96 +/- 0.12, p < 0.001) aquaporin-3 (0.31 +/- 0.12 vs. 0.93 +/- 0.20, p < 0.001), and Na-K-ATPase-alpha (0.39 +/- 0.08 vs. 0.92 +/- 0.12, p < 0.001), whereas intratracheal salbutamol increased the gene expression of aquaporin-1 (0.46 +/- 0.11 vs. 0.92 +/- 0.06, p < 0.001) and Na-K-ATPase-alpha (0.32 +/- 0.07 vs. 0.58 +/- 0.15, p < 0.001). In ARDSexp, the gene expression of ion channels and aquaporin was not influenced by salbutamol. Morphological and functional variables and edema formation were not affected by salbutamol in any of the ARDS groups, regardless of the route of administration. CONCLUSION: Salbutamol administration increased the expression of alveolar epithelial ion channels and aquaporin in mild ARDSp, but not ARDSexp, with no effects on lung morphology and function or edema formation. These results may contribute to explain the negative effects of beta2-agonists on clinical outcome in ARDS

The Marine Isotopic Stage 3 (MIS 3) in Valleys of the Undulated Pampa, Buenos Aires Province, Argentina

A depositional unit called DU2 identified for the period MIS 3 (ca- 30,000-60,000 yr. B.P.) formed by only one sedimentary facies (F3) was found in the Luján and Salto-Arrecifes rivers basins. F3 is a fluvio?lacustrine unit that overlies in erosive unconformity over eolian sediments with ages of 56,400 ± 6,500 and 50,400 ± 10,200 years B.A. and is unconformably covered by another eolian vitroclastic sandy loess deposit, dated as 32,000 ±4,000 years (Infrared Stimulated Luminescence, IRSL) (Blasi, et al. 2010). It represents the recurrence of ephemeral fluvial streamlets and the development of temporary pools by subsequent damming of channels. It corresponds lithologically to sandy muddy gravel, gravelly muddy sand, gravelly mud, olive to pale olive feldspar and quartz sands, bearing extinct mollusks such as Heleobia ameghini and Diplodon lujanensis. Radiocarbon chronologies obtained on monospecific samples of Cyprideis salebrosa hartmanni and Heleobia ameghini yielded ages of 37,710 ± 840 years 14C B.P. and >40,000 years 14C B.P., respectively. Furthermore, the age obtained through the IRSL technique was of 44,000 ± 6,500 years. Based upon the analyzed bioproxies (malacological, phytoliths and diatomological content) F3 accumulated under variable climatic conditions, ranging from temperate to colder and from subhumid to drier. According to the exhaustive stratigraphic identification, it is proposed that in NE Buenos Aires Province, the so-called Undulated Pampa region, the sediments that were accumulated during MIS3 occur only in the central portion of the studied fluvial basins. This prompted two hypotheses related to the existence of a particular drainage pattern for the Late Pleistocene, different from the present one, and subsequent tectonic controls that allowed the identification of DU2 sediments only in some of the analyzed sections.Fil: Blasi, Adriana María. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. División Mineralogía y Petrología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Castiñeira Latorre, Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. División Mineralogía y Petrología; ArgentinaFil: Cusminsky, Gabriela Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Carignano, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Paleozoología Invertebrados; Argentin