New insights into the mechanism of DNA mismatch repair

The genome of all organisms is constantly being challenged by endogenous and exogenous sources of DNA damage. Errors like base:base mismatches or small insertions and deletions, primarily introduced by DNA polymerases during DNA replication are repaired by an evolutionary conserved DNA mismatch repair (MMR) system. The MMR system, together with the DNA replication machinery, promote repair by an excision and resynthesis mechanism during or after DNA replication, increasing replication fidelity by upto-three orders of magnitude. Consequently, inactivation of MMR genes results in elevated mutation rates that can lead to increased cancer susceptibility in humans. In this review, we summarize our current understanding of MMR with a focus on the different MMR protein complexes, their function and structure. We also discuss how recent findings have provided new insights in the spatio-temporal regulation and mechanism of MMR

Measurement of charged jet suppression in Pb-Pb collisions at sNN\sqrt{s_{NN}}=2.76TeV

A measurement of the transverse momentum spectra of jets in Pb-Pb collisions at $\sqrt{s_{NN}}$=2.76TeV is reported. Jets are reconstructed from charged particles using the anti-$k_T$ jet algorithm with jet resolution parameters R of 0.2 and 0.3 in pseudo-rapidity |$\eta$|<0.5. The transverse momentum p_T of charged particles is measured down to 0.15 GeV/c which gives access to the low p_T fragments of the jet. Jets found in heavy-ion collisions are corrected event-by-event for average background density and on an inclusive basis (via unfolding) for residual background fluctuations and detector effects. A strong suppression of jet production in central events with respect to peripheral events is observed. The suppression is found to be similar to the suppression of charged hadrons, which suggests that substantial energy is radiated at angles larger than the jet resolution parameter R=0.3 considered in the analysis. The fragmentation bias introduced by selecting jets with a high p_T leading particle, which rejects jets with a soft fragmentation pattern, has a similar effect on the jet yield for central and peripheral events. The ratio of jet spectra with R=0.2 and R=0.3 is found to be similar in Pb-Pb and simulated PYTHIA pp events, indicating no strong broadening of the radial jet structure in the reconstructed jets with R<0.3.A measurement of the transverse momentum spectra of jets in Pb-Pb collisions at $\sqrt{s_{\rm NN}}=2.76$ TeV is reported. Jets are reconstructed from charged particles using the anti-$k_{\rm T}$ jet algorithm with jet resolution parameters $R$ of $0.2$ and $0.3$ in pseudo-rapidity $|\eta|<0.5$. The transverse momentum $p_{\rm T}$ of charged particles is measured down to $0.15$ GeV/$c$ which gives access to the low $p_{\rm T}$ fragments of the jet. Jets found in heavy-ion collisions are corrected event-by-event for average background density and on an inclusive basis (via unfolding) for residual background fluctuations and detector effects. A strong suppression of jet production in central events with respect to peripheral events is observed. The suppression is found to be similar to the suppression of charged hadrons, which suggests that substantial energy is radiated at angles larger than the jet resolution parameter $R=0.3$ considered in the analysis. The fragmentation bias introduced by selecting jets with a high $p_{\rm T}$ leading particle, which rejects jets with a soft fragmentation pattern, has a similar effect on the jet yield for central and peripheral events. The ratio of jet spectra with $R=0.2$ and $R=0.3$ is found to be similar in Pb-Pb and simulated PYTHIA pp events, indicating no strong broadening of the radial jet structure in the reconstructed jets with $R<0.3$