Mapping of the Gynoecy in Bitter Gourd (<i>Momordica charantia</i>) Using RAD-Seq Analysis

<div><p><i>Momordica charantia</i> is a monoecious plant of the Cucurbitaceae family that has both male and female unisexual flowers. Its unique gynoecious line, OHB61-5, is essential as a maternal parent in the production of F₁ cultivars. To identify the DNA markers for this gynoecy, a RAD-seq (restriction-associated DNA tag sequencing) analysis was employed to reveal genome-wide DNA polymorphisms and to genotype the F₂ progeny from a cross between OHB61-5 and a monoecious line. Based on a RAD-seq analysis of F₂ individuals, a linkage map was constructed using 552 co-dominant markers. In addition, after analyzing the pooled genomic DNA from monoecious or gynoecious F₂ plants, several SNP loci that are genetically linked to gynoecy were identified. GTFL-1, the closest SNP locus to the putative gynoecious locus, was converted to a conventional DNA marker using invader assay technology, which is applicable to the marker-assisted selection of gynoecy in <i>M. charantia</i> breeding.</p></div