Six artificial recharge pilot replicates to gain insight into water quality enhancement processes

The processes that control water quality improvement during artificial recharge (filtering, degradation, and adsorption) can be enhanced by adding a reactive barrier containing different types of sorption sites and promoting diverse redox states along the flow path, which increases the range of pollutants degraded. While this option looks attractive for renaturazing reclaimed water, three issues have to be analyzed prior to broad scale application: (1) a fair comparison between the system with and without reactive barrier; (2) the role of plants in prevention of clogging and addition of organic carbon; and (3) the removal of pathogens. Here, we describe a pilot installation built to address these issues within a waste water treatment plant that feeds on water reclaimed from the secondary outflow. The installation consists of six systems of recharge basin and aquifer with some variations in the design of the reactive barrier and the heterogeneity of the aquifer. We report preliminary results after one year of operation. We find that (1) the systems are efficient in obtaining a broad range of redox conditions (at least iron and manganese reducing), (2) contaminants of emerging concern are significantly removed (around 80% removal, but very sensitive to the compound), (3) pathogen indicators (E. coli and Enterococci) drop by some 3–5 log units, and (4) the recharge systems maintained infiltration capacity after one year of operation (only the system without plants and the one without reactive barrier displayed some clogging). Overall, the reactive barrier enhances somewhat the performance of the system, but the gain is not dramatic, which suggests that barrier composition needs to be improved.This study was supported by the Water Joint Programming Initiative (JPI)Water Challenges for a ChangingWorld 2014 through the project ACWAPUR (ACcelerated Water PURification during artificial recharge of aquifers: A tool to restore drinking water sources) and partially funded by the Spanish Ministry of Science, Innovation and Universities through PCIN-2015-245. We are very grateful to the staff of ‘Aguas de la Costa Brava' for their assistance, and to Ma Pau Serra-Roig and Ana Julia Acunha for their contribution to the chemical analysis and to Juli_a Garcia for his effort with the field site work.Peer ReviewedPostprint (published version

Reactive barriers for renaturalization of reclaimed water during soil aquifer treatment

Managed aquifer recharge (MAR) is known to increase available water quantity and to improve water quality. However, its implementation is hindered by the concern of polluting aquifers, which might lead to onerous treatment and regulatory requirements for the source water. These requirements might make MAR unsustainable both economically and energetically. To address these concerns, we tested reactive barriers laid at the bottom of infiltration basins to enhance water quality improvement during soil passage. The goal of the barriers was to (1) provide a range of sorption sites to favor the retention of chemical contaminants and pathogens; (2) favor the development of a sequence of redox states to promote the degradation of the most recalcitrant chemical contaminants; and (3) promote the growth of plants both to reduce clogging, and to supply organic carbon and sorption sites. We summarized our experience to show that the barriers did enhance the removal of organic pollutants of concern (e.g., pharmaceuticals and personal care products). However, the barriers did not increase the removal of pathogens beyond traditional MAR systems. We reviewed the literature to suggest improvements on the design of the system to improve pathogen attenuation and to address antibiotic resistance gene transfer.This research was funded by the Spanish Ministry of Science and Innovation CEX2018-000794-S), Water JPI (MARadentro-PCI2019-103603) and Catalan Water Agency (RESTORA-CA210/18/00040).Peer ReviewedPostprint (published version

Water Quality and Total Microbial Load: A Double-Threshold Identification Procedure Intended for Space Applications

During longer-lasting future space missions, water renewal by ground-loaded supplies will become increasingly expensive and unmanageable for months. Space exploration by self-sufficient spacecrafts is thus demanding the development of culture-independent microbiological methods for in-flight water monitoring to counteract possible contamination risks. In this study, we aimed at evaluating total microbial load data assessed by selected early-warning techniques with current or promising perspectives for space applications (i.e., HPC, ATP-metry, qPCR, flow cytometry), through the analysis of water sources with constitutively different contamination levels (i.e., chlorinated and unchlorinated tap waters, groundwaters, river waters, wastewaters). Using a data-driven double-threshold identification procedure, we presented new reference values of water quality based on the assessment of the total microbial load. Our approach is suitable to provide an immediate alert of microbial load peaks, thus enhancing the crew responsiveness in case of unexpected events due to water contamination and treatment failure. Finally, the backbone dataset could help in managing water quality and monitoring issues for both space and Earth-based applications

Microbial colonization patterns and biodegradation of petrochemical and biodegradable plastics in lake waters: insights from a field experiment

IntroductionOnce dispersed in water, plastic materials become promptly colonized by biofilm-forming microorganisms, commonly known as plastisphere.MethodsBy combining DNA sequencing and Confocal Laser Scanning Microscopy (CLSM), we investigated the plastisphere colonization patterns following exposure to natural lake waters (up to 77 days) of either petrochemical or biodegradable plastic materials (low density polyethylene - LDPE, polyethylene terephthalate - PET, polylactic acid - PLA, and the starch-based MaterBi® - Mb) in comparison to planktonic community composition. Chemical composition, water wettability, and morphology of plastic surfaces were evaluated, through Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM), and static contact angle analysis, to assess the possible effects of microbial colonization and biodegradation activity.Results and DiscussionThe phylogenetic composition of plastisphere and planktonic communities was notably different. Pioneering microbial colonisers, likely selected from lake waters, were found associated with all plastic materials, along with a core of more than 30 abundant bacterial families associated with all polymers. The different plastic materials, either derived from petrochemical hydrocarbons (i.e., LDPE and PET) or biodegradable (PLA and Mb), were used by opportunistic aquatic microorganisms as adhesion surfaces rather than carbon sources. The Mb-associated microorganisms (i.e. mostly members of the family Burkholderiaceae) were likely able to degrade the starch residues on the polymer surfaces, although the Mb matrix maintained its original chemical structure and morphology. Overall, our findings provide insights into the complex interactions between aquatic microorganisms and plastic materials found in lake waters, highlighting the importance of understanding the plastisphere dynamics to better manage the fate of plastic debris in the environment

Modeling substrate interactions during aerobic biodegradation of mixtures of vinyl chloride and ethene

Ethene (ETH) is often associated with vinyl chloride (VC) in contaminated groundwater, as it is formed along with vinyl chloride during reductive dechlorination of higher chloroethenes (e.g., perchloroethylene and trichloroethylene). In the present study the interaction between VC and ETH during their aerobic biodegradation by enrichment cultures was investigated. The cultures were able to use both compounds as growth substrates. In mixture experiments, the degradation rate of one substrate was affected by the presence of the other. A biokinetic model based on competitive inhibition described well the observed substrate interactions over a range of initial VC (0-144 mu mol L-1) and ETH (0-37.5 mu mol L-1) concentrations, using parameters estimated from single-substrate experiments. Notably, half-velocity coefficients could be used as competitive inhibition coefficients. This finding shows the importance of obtaining accurate measurements of half-velocity coefficients in order model competitive inhibition processes. Simulation results showed that when the initial ETH concentration was raised from 0 to 30 mu mol L-1, the apparent half-velocity coefficient for VC (K-VC(APP)) increased by nearly three times, from 12.9 to 35.4 mu mol L-1. This finding has strong environmental implications because a low half-velocity coefficient for VC is regarded as the major prerequisite for achieving efficient and complete VC degradation. Moreover, the effect of ETH on the efficiency of VC removal is strongly dependent on the K-VC/K-ETH ratio, consequently determination of K-ETH for VC-degrading microbes is important when biodegradation (or bioaugmentation) is considered for clean up of VC-contaminated sites. Additional model simulations, using the ratio of K-VC to K-ETH for previously characterized VC- and ETH-utilizing microorganisms (values ranged from 0.06 to 1.2) showed that their ability to degrade VC in the presence of ETH may differ significantly

Effect of periodic feeding on substrate uptake and storage rates by a pure culture of Thiothrix (CT3 strain)

A pure culture of Thiothrix strain CT3 has been aerobically cultured under periodic acetate feeding in a Sequencing Batch Reactor (SBR) at volumetric organic load rate of 0.12 gCOD L-1 d(-1). Two different culture residence times (12d or 20d) were adopted as well as two different feed frequencies (1 and 4d(-1), for each culture residence time), the volumetric organic load rate being the same under all conditions. The transient response of the microorganism to the periodic acetate feed was investigated through batch tests with biomass withdrawn from the SBR, as function of the different SBR operating conditions. In all tested conditions, a quick transient response to the acetate spike was observed with fast increase of acetate uptake rate (ranging from 71 to 247 mgCOD gCOD(-1) h(-1)). This transient response was mainly due to acetate storage in form of poly-hydroxybutyrate (ranging from 45% to 64% of the observed yield) whereas the growth response (i.e. increase of production rate of active biomass) generally played a minor role (ranging from 21% to 38% of the observed yield). Apart from this general trend, culture residence time as well as feed frequency had a strong impact on transient behaviour of cultured cells. The overall transient response (i.e. maximum specific substrate removal rate) increased as culture residence time decreased or as feed frequency increased. moreover, the ratio of storage response and growth response increased as the overall transient response decreased, i.e. the storage response was preferentially maintained when cells presented a lower transient response. The ability of the cells to increase their growth rate with respect to SBR average value was the lowest under the most unfavourable conditions (residence time 20 El, feed frequency 1 d-1) and increased with the increase in maximum substrate uptake rate. (c) 2006 Elsevier Ltd. All rights reserved

Six artificial recharge pilot replicates to gain insight into water quality enhancement processes

The processes that control water quality improvement during artificial recharge (filtering, degradation, and adsorption) can be enhanced by adding a reactive barrier containing different types of sorption sites and promoting diverse redox states along the flow path, which increases the range of pollutants degraded. While this option looks attractive for renaturazing reclaimed water, three issues have to be analyzed prior to broad scale application: (1) a fair comparison between the system with and without reactive barrier; (2) the role of plants in prevention of clogging and addition of organic carbon; and (3) the removal of pathogens. Here, we describe a pilot installation built to address these issues within a waste water treatment plant that feeds on water reclaimed from the secondary outflow. The installation consists of six systems of recharge basin and aquifer with some variations in the design of the reactive barrier and the heterogeneity of the aquifer. We report preliminary results after one year of operation. We find that (1) the systems are efficient in obtaining a broad range of redox conditions (at least iron and manganese reducing), (2) contaminants of emerging concern are significantly removed (around 80% removal, but very sensitive to the compound), (3) pathogen indicators (E. coli and Enterococci) drop by some 3–5 log units, and (4) the recharge systems maintained infiltration capacity after one year of operation (only the system without plants and the one without reactive barrier displayed some clogging). Overall, the reactive barrier enhances somewhat the performance of the system, but the gain is not dramatic, which suggests that barrier composition needs to be improved. © 2019 The AuthorsThis study was supported by the Water Joint Programming Initiative (JPI) Water Challenges for a Changing World 2014 through the project ACWAPUR (ACcelerated Water PURification during artificial recharge of aquifers: A tool to restore drinking water sources) and partially funded by the Spanish Ministry of Science, Innovation and Universities through PCIN-2015-245 . We are very grateful to the staff of ‘Aguas de la Costa Brava' for their assistance, and to M a Pau Serra-Roig and Ana Julia Acunha for their contribution to the chemical analysis and to Juliá Garcia for his effort with the field site work.Peer reviewe