Recent advances in systemic therapy: Advances in systemic therapy for HER2-positive metastatic breast cancer

Human epidermal growth factor receptor (HER)2 over-expression is associated with a shortened disease-free interval and poor survival. Although the addition of trastuzumab to chemotherapy in the first-line setting has improved response rates, progression-free survival, and overall survival, response rates declined when trastuzumab was used beyond the first-line setting because of multiple mechanisms of resistance. Studies have demonstrated the clinical utility of continuing trastuzumab beyond progression, and further trials to explore this concept are ongoing. New tyrosine kinase inhibitors, monoclonal antibodies, PTEN (phosphatase and tensin homolog) pathway regulators, HER2 antibody-drug conjugates, and inhibitors of heat shock protein-90 are being evaluated to determine whether they may have a role to play in treating trastuzumab-resistant metastatic breast cancer

Contraceptive use and method among immigrant women in France: Relationship with socioeconomic status

[[abstract]]Objectives:We investigated the relationship between socioeconomic status and the use and method of contraception among immigrant, second-generation immigrant, and non-immigrant women in France. Methods: We analysed data from the 2008-2009 survey 'Trajectories and origins: Survey on the diversity of populations in France'. A total of 7070 women aged 18 to 45 years were identified, and information concerning contraceptive use and choice was obtained by self-report. Polytomous logistic regression models were used to assess association estimates. Results: Recent contraceptive use among immigrant and second-generation immigrant women was significantly lower than that of non-immigrant women. Lower educational attainment and unemployment were associated with an estimated 31̃59% reduction in odds of contraceptive use for immigrant and second-generation immigrant women; however, this was not the case for non-immigrant women. Among the latter, lower educational attainment appeared to be associated with increased use of oral contraceptives. Conclusions Our findings suggest the need to advance our understanding of potential barriers to contraceptives created by socioeconomic forces across different societal/cultural contexts

Gene expression in coffee

Coffee is cultivated in more than 70 countries of the intertropical belt where it has important economic, social and environmental impacts. As for many other crops, the development of molecular biology technics allowed to launch research projects for coffee analyzing gene expression. In the 90s decade, the first expression studies were performed by Northern-blot or PCR, and focused on genes coding enzymes of the main compounds (e.g., storage proteins, sugars, complex polysaccharides, caffeine and chlorogenic acids) found in green beans. Few years after, the development of 454 pyrosequencing technics generated expressed sequence tags (ESTs) obviously from beans but also from other organs (e.g., leaves and roots) of the two main cultivated coffee species, Coffea arabica and C. canephora. Together with the use of real-time quantitative PCR, these ESTs significantly raised the number of coffee gene expression studies leading to the identification of (1) key genes of biochemical pathways, (2) candidate genes involved in biotic and abiotic stresses as well as (3) molecular markers essential to assess the genetic diversity of the Coffea genus, for example. The development of more recent Illumina sequencing technology now allows large-scale transcriptome analysis in coffee plants and opens the way to analyze the effects on gene expression of complex biological processes like genotype and environment interactions, heterosis and gene regulation in polypoid context like in C. arabica. The aim of the present review is to make an extensive list of coffee genes studied and also to perform an inventory of large-scale sequencing (RNAseq) projects already done or on-going