Catalytic determination of cobalt by flow injection spectrophotometry: application in animal faeces analysis using hydrochloric acid as extracting solution

A flow injection system for cobalt determination in faeces of bovines and equines receiving Co-EDTA as a tracer in animal nutrition studies is proposed, using 1.0 mol HCl l(-1) as extracting medium, and detection by flow injection spectrophotometric procedure based on the catalytic effect of Co(II) on the oxidation of Tiron by hydrogen peroxide. Linearity was observed up to 8.0 mu g Co l(-1), and the detection limit was 0.2 mu g Co l(-1) (3 s blank). The system requires 0.12 mg Tiron per determination and handles 65 samples h(-1). No baseline drift was observed during extended operation periods (8 h) and precise results (r.s.d.<l%) were detected. Accuracy was confirmed by running several faeces samples already analysed by flame atomic absorption spectrometry. Results related to the HCl extraction procedure were in close agreement with those obtained using the nitric-perchloric digestion (y=0.978x+0.101, R-2=0.9848), suggesting that HCl extraction and flow injection determination can be used routinely for rapid analysis of cobalt in faeces, (C) 1998 Elsevier Science B.V.741798

Isolation and characterization of microsatellite loci in Paspalum notatum Flugg, (Poaceae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Paspalum notatum is a forage grass recognized as one of the major constituents of the native grasslands in the New World. The knowledge of the genetic diversity and structure of P. notatum populations is fundamental for the conservation and germplasm management of this species. About 11 microsatellite markers were isolated from P. notatum and characterized in 25 accessions. The average number of alleles per locus was 7.9 and the PIC ranged from 0.36 to 0.89. The data demonstrated that the most of markers are suitable to detect polymorphism and to study the genetic diversity in the P. notatum species. Moreover, the transferability of these microsatellite were tested on other three congeneric species.10619771980Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

MICROSATELLITE LOCI FOR PASPALUM ATRATUM (POACEAE) AND CROSS-AMPLIFICATION IN OTHER SPECIES

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Premise of the study: Paspalum atratum is a perennial, cespitose, tropical grass native to Central and South America. This species belongs to a polyploid complex (Plicatula group) little known at the genetic level. The characterized microsatellite markers provide new informative tools for further studies of the hybridization, mating systems, and structure of the population. Methods and Results: Using the microsatellite-enriched library method, we isolated and characterized 19 microsatellite markers from P. atratum. Eleven of them were polymorphic, showing a variable degree of variation, while eight were monomorphic in the samples analyzed. Additionally, the transferability of these microsatellite markers was tested in other species. Conclusions: These results suggest that the characterized markers have enough discriminatory potential to be used in genetic characterizations of Paspalum taxa, which are based on an understanding of their mating systems and genetic structure, as well as in understanding the evolutionary processes involved in the evolution of groups of Paspalum.9711E107E110Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)[2006/61242-8]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP [05/51010][2006/61242-8