Isolation and properties of lipolysis inhibitory proteins from wheat germ and wheat bran

International audienc

Gastric lipase: Evidence of an adaptive response to dietary fat in the rabbit

International audienc

Effets de produits céréaliers sur l'activité in vitro de la lipase pancréatique et première caractérisation d'un inhibiteur protéique de l'enzyme dans les céréales

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Output of liver fatty acid-binding protein (L-FABP) in bile.

International audienceLiver fatty acid-binding protein (L-FABP) is a small cytoplasmic molecule highly expressed in the liver. Since L-FABP exhibits affinities for several biliary components, its presence in bile was explored by Western blotting and competitive ELISA in various mammalian species. A L-FABP-like immunoreactivity was consistently found in both hepatic and gallbladder bile. A close molecular identity between this 14 kDa biliary protein and the purified L-FABP was assessed by immunological analyses and high performance capillary electrophoresis. Pharmacological induction of hepatic L-FABP biosynthesis led to a similar increase in biliary L-FABP levels showing a close relationships between the cytosolic and biliary contents of this protein. Finally, a correlation between the presence of L-FABP in bile and both bile flow and bile acid release was found. These data suggest an output of L-FABP in bile in normal conditions which might be coupled with the physiological release of biliary components

Adaptation of Lingual Lipase to Dietary Fat in Rats

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Monoglycoconjugated phthalocyanines: effect of sugar and linkage on photodynamic activity.

International audienceBACKGROUND: Click chemistry can be advantageously used to graft carbohydrates on phthalocyanines which are potent photosensitisers, but the effect of the presence of triazole moieties on photodynamic efficiency was not investigated systematically to date. The nature and linkage of the sugar were investigated in order to define structure-activity relationships. METHOD: Two sets of monoglycoconjugated water-soluble phthalocyanines have been designed and their photodynamic activity and uptake investigated in HT-29 human colon adenocarcinoma cells. Carbohydrates: galactose, mannose or lactose were grafted onto Zn(II) phthalocyanines either by glycosylation or by click reaction. RESULTS: The triazole linkage formed by click conjugation lowered the biological efficiency for mannose and galactose, compared to classical glycosylation grafting. The mannose conjugate formed by glycosylation was the most photodynamically active, without correlation with the photosensitiser cell uptake

Influence de régimes hyperlipidiques sur les relations entre cholestérol libre des HDL et des LDL et cholestérol et sels biliaires sécrétés dans la bile de rats

International audienc

Digestion and Absorption of Tube-Feeding Emulsions With Different Droplet Sizes and Compositions in the Rat

International audienc

Hydrolysis of emulsions with different triglycerides and droplet sizes by gastric lipase in vitro. Effect on pancreatic lipase activity

International audienc