Secretome Data from Trichoderma Reesei and Aspergillus Niger Cultivated in Submerged and Sequential Fermentation Methods

The cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. The proteomic analysis data presented here provide critical informa- tion to compare proteins secreted by Trichoderma reesei and Aspergillus niger when cultivated through submerged and sequential fermenta- tion processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. The proteins were organized according to the families described in CAZy database as cellulases, hemicellulases, proteases/peptidases, cell-wall-protein, lipases, others (catalase, ester- ase, etc.), glycoside hydrolases families, predicted and hypothetical proteins. Further detailed analysis of this data is provided in “Secre- tome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation process: enzyme production for sugarcane bagasse hydrolysis” C. Florencio, F.M. Cunha, A.C Badino, C.S. Farinas, E. Ximenes, M.R. Ladisch (2016) [1]

Transitional justice and the political 'work' of domestic tourism

The relationship between tourism and transitional justice is little-researched. This paper explores the importance of domestic tourism for enabling citizens to encounter and engage with wider transitional justice projects. This issue is explored with reference to a memorial museum in Romania which interprets political violence and state repression. Semi-structured interviews with 52 domestic tourists were undertaken (using purposive sampling to select participants) and the interviews were analysed using thematic analysis. Most visitors reported general sightseeing motives for visiting the museum and may not have anticipated engaging with transitional justice messages. However, their engagement went beyond sightseeing in a range of ways. Visitors participated in acts of memory-work, acknowledged the victims of repression, and recognized the core message of transitional justice – “never again”. They also reflected on the relationship between the recent past and the present, and recognized the role of the museum as a resource for future generations. These experiences were shared by those who had lived through state repression and those who had not. The findings indicate that domestic tourism is a meaningful but overlooked context through which citizens can engage with broader transitional justice projects

July 2004 Report of Progress

Progress of each ALS-NSCORT project given by each project lead. 10 pages

Herpes-Virus Infection in Patients with Langerhans Cell Histiocytosis: A Case-Controlled Sero-Epidemiological Study, and In Situ Analysis

BACKGROUND: Langerhans cell histiocytosis (LCH) is a rare disease that affects mainly young children, and which features granulomas containing Langerhans-type dendritic cells. The role of several human herpesviruses (HHV) in the pathogenesis of LCH was suggested by numerous reports but remains debated. Epstein-barr virus (EBV, HHV-4), & Cytomegalovirus (CMV, HHV-5) can infect Langerhans cells, and EBV, CMV and HHV-6 have been proposed to be associated with LCH based on the detection of these viruses in clinical samples. METHODOLOGY: We have investigated the prevalence of EBV, CMV and HHV-6 infection, the characters of antibody response and the plasma viral load in a cohort of 83 patients and 236 age-matched controls, and the presence and cellular localization of the viruses in LCH tissue samples from 19 patients. PRINCIPAL FINDINGS: The results show that prevalence, serological titers, and viral load for EBV, CMV and HHV-6 did not differ between patients and controls. EBV was found by PCR in tumoral sample from 3/19 patients, however, EBV small RNAs EBERs -when positive-, were detected by in situ double staining in bystander B CD20+ CD79a+ lymphocytes and not in CD1a+ LC. HHV-6 genome was detected in the biopsies of 5/19 patients with low copy number and viral Ag could not be detected in biopsies. CMV was not detected by PCR in this series. CONCLUSIONS/SIGNIFICANCE: Therefore, our findings do not support the hypothesis of a role of EBV, CMV, or HHV-6 in the pathogenesis of LCH, and indicate that the frequent detection of Epstein-barr virus (EBV) in Langerhans cell histiocytosis is accounted for by the infection of bystander B lymphocytes in LCH granuloma. The latter observation can be attributed to the immunosuppressive micro environment found in LCH granuloma

Aptamers for pharmaceuticals and their application in environmental analytics

Aptamers are single-stranded DNA or RNA oligonucleotides, which are able to bind with high affinity and specificity to their target. This property is used for a multitude of applications, for instance as molecular recognition elements in biosensors and other assays. Biosensor application of aptamers offers the possibility for fast and easy detection of environmental relevant substances. Pharmaceutical residues, deriving from human or animal medical treatment, are found in surface, ground, and drinking water. At least the whole range of frequently administered drugs can be detected in noticeable concentrations. Biosensors and assays based on aptamers as specific recognition elements are very convenient for this application because aptamer development is possible for toxic targets. Commonly used biological receptors for biosensors like enzymes or antibodies are mostly unavailable for the detection of pharmaceuticals. This review describes the research activities of aptamer and sensor developments for pharmaceutical detection, with focus on environmental applications

February 2005 Report of Progress

Progress of each ALS-NSCORT project given by each project lead. 8 pages

May 2004 Report of Progress

Progress of each ALS-NSCORT project given by each project lead. 11 pages