1,477 research outputs found
Testicular activity and sperm glycoproteins in giant red shrimp Aristaeomorpha foliacea
The reproduction of male giani red shrimp Aristaeomorpha foliacea, collected from thè late
winterto thè summer in thè north-western lonian Sea (Mediterranean Sea), was investigated using
histological and histochemistry methods. Seasonal changes in thè spermiogenesis and thè glycoprotein
pattern were found and sperm glycoproteins matured as gametes moved from thè testis to
thè terminal ampliila. In serial sections stained with hematoxylin and eosin thè testicular activity
appeared to be discontinuous. In late winter thè testes had no meiotic activity and thè seminiferous
epithelium consisted of interkinetic spermatogonia and spermatozoa. In spring, spermiogenetic
activity was high and thè seminiferous epithelium mainly consisted of spermatocytes and spermatozoa
while in summer, thè testes were again inactive since both spermatocytes and spermatozoa
were lacking. The use of twelve different lectins indicated that thè intratesticular spermatozoa
from late winter to summer contain surface binding sites for SNA, MAA, Con A and KOH-sialidase
(si)-WGA. In March and July they also exhibited nuclear and cytoplasmic reactivity for SNA and
Con A. In thè hemispermatophore thè spermatozoa displayed a more complex lectin-binding pattern
because they also reacted with PNA, DBA, HPA, OSA II. The staining with DBA, KOH-si-
DBA, and OSA II showed differences between thè spermatozoa from late winter-spring
hemispermatophores and summer hemispermatophores: thè former showed a nuclear affinity
whereas thè latter displayed surface and/or cytoplasm staining. No reaction was observed with
SBA, GSAI-B4, UEA I, and LTA
Avaliacoes da infeccao de explantes de feijoeiro por Agrobacterium tumefaciens por meio de microscopia.
Phaseolus vulgaris L. e considerada recalcitrante a transformacao por Agrobacterium tumefaciens. Contudo, alteracoes no meio de co-cultivo, utilizacao de linhagens hipervirulentas de Agrobacterium e de vetores binarios contendo genes vir demostraram que o feijoeiro e susceptivel a essa bacteria. O objetivo do presente trabalho foi estudar o efeito da sonificacao nos tecidos vegetais de feijoeiro, bem como a penetracao da Agrobacterium nas camadas subepidermicas do tecido vegetal, usando a metodologia SAAT ("Sonification-Assisted Agrobacterium-mediated Transformation"). A variedade de feijoeiro utilizada foi a Olathe Pinto, a linhagem de A. tumefaciens foi LBA4404:pTOK.Os embrioes de feijao foram pre-tratados po 14 dias em meio de multibrotacao e, entao submetidos a sonificacao (de 0 ou 60 segundos) na presenca de Agrobacterium. Apos a inoculacao foram co-cultivados por 24 horas em meio liquido seguido de 48 horas em meio solido, ambos, contendo 20 m. L -1 de acetoceringona. Os explantes inoculados foram fixadas em solucao de Karnovsk para avaliacoes em microscopia optica e eletronica de varredura. As analises da microspia demostraram a presenca de rupturas na epiderme, quebras da parede celular e invasao da Agrobacterium nos tecidos subepidermicos. Os reultados demostraram que o metodo SAAT e uma tecnica viavel para a inoculcao de Agrobacterium em explantes de P. vulgaris.bitstream/CPAC-2010/23710/1/bolpd-04.pd
Acceleration with Self-Injection for an All-Optical Radiation Source at LNF
We discuss a new compact gamma-ray source aiming at high spectral density, up
to two orders of magnitude higher than currently available bremsstrahlung
sources, and conceptually similar to Compton Sources based on conventional
linear accelerators. This new source exploits electron bunches from
laser-driven electron acceleration in the so-called self-injection scheme and
uses a counter-propagating laser pulse to obtain X and gamma-ray emission via
Thomson/Compton scattering. The proposed experimental configuration inherently
provides a unique test-bed for studies of fundamental open issues of
electrodynamics. In view of this, a preliminary discussion of recent results on
self-injection with the FLAME laser is also given.Comment: 8 pages, 10 figures, 44 references - Channeling 2012 conferenc
Prospects and Pitfalls of Desalination Development: Insights From Three States
Like Coleridge’s sailor in the Rime of the Ancient Mariner, burgeoning coastal communities in the United States have long lamented the fact that while situated by vast expanses of ocean water, they could not slake their growing thirst with it. Increasing populations, steady demand, and declining. Freshwater sources have amplified the problem across the U.S., particularly in the country’s three most populous states: California, Texas, and Florida. This paper examines efforts in three states to bring desalination plants online to transform saline water into a secure water supply
Incomplete ileocecal bypass for ileal pathology in horses: 21 cases (2012–2019)
Background: Incomplete ileocecal bypass can be performed in cases in which an ileal disfunction is suspected but resection of the diseased ileum is not necessary. Objectives: To describe the clinical findings, the surgical technique, and the outcome of 21 cases of colic with ileal pathologies that underwent an incomplete ileocecal bypass. Methods: Historical, clinical, and surgical features of cases diagnosed with pathologies involving the ileum or the ileocecal valve that underwent ileocecal anastomosis without ileal resection were retrieved. Clinical (heart rate, duration of symptoms, presence of reflux, age, weight at arrival) and surgical (surgical pathology, duration of surgery, type of anastomosis) data were retrieved and analysed. Data on short term survival and postoperative complications (colic, post-operative reflux, incisional infection, fever), length of hospital stay, and long term follow up were also obtained. Results: A total of 21 horses met the criteria; 13 horses had ileal impaction (one with muscular hypertrophy), 5 horses had epiploic foramen entrapment, and 3 horses had a pedunculated lipoma. An incomplete ileocecal bypass was performed with a two-layer hand-sewn side-to-side technique in 19 cases and with a stapled side-to-side technique in 2 cases. Short term survival was 95.2%. At 12-months follow up, all horses but two were alive, and 13 of the 14 sport horses returned to their previous level of activity. Long term survival was 90.47%. Conclusions Incomplete ileocecal bypass may represent a valid surgical technique in case of ileocecal valve disfunction when ileum resection is not necessary; this technique may represent an alternative to extensive manipulation without subsequent recurrence of ileal impaction
Bone mineral density in patients on home parenteral nutrition: a follow-up study.
Home artificial nutrition
An Overview of the Obese-Asthma Phenotype in Children
Asthma is the most common chronic disease in childhood. Overweight and obesity are included among the comorbidities considered in patients with difficult-to-treat asthma, suggesting a specific phenotype of the disease. Therefore, the constant increase in obesity prevalence in children and adolescents raises concerns about the parallel increase of obesity-associated asthma. The possible correlation between obesity and asthma has been investigated over the last decade by different authors, who suggest a complex multifactorial relationship. Although the particular non-eosinophilic endotype of obesity-related asthma supports the concept that high body weight precedes asthma development, there is ongoing debate about the direct causality of these two entities. A number of mechanisms may be involved in asthma in combination with obesity disease in children, including reduced physical activity, abnormal ventilation, chronic systemic inflammation, hormonal influences, genetics and additional comorbidities, such as gastroesophageal reflux and dysfunctional breathing. The identification of the obesity-related asthma phenotype is crucial to initiate specific therapeutic management. Besides the cornerstones of asthma treatment, lifestyle should be optimized, with interventions aiming to promote physical exercise, healthy diet, and comorbidities. Future studies should clarify the exact association between asthma and obesity and the mechanisms underlying the pathogenesis of these two related conditions with the aim to define personalized therapeutic strategies for asthma management in this population
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