Proteomic profiling of human amnion for preterm birth biomarker discovery

Spontaneous preterm birth (PTB) complicates about 12% of pregnancies worldwide, remaining the main cause of neonatal morbidity and mortality. Spontaneous preterm birth PTBs is often caused by microbial-induced preterm labor, mediated by an inflammatory process threatening both maternal and newborn health. In search for novel predictive biomarkers of PTB and preterm prelabor rupture of the membranes (pPROM), and to improve understanding of infection related PTB, we performed an untargeted mass spectrometry discovery study on 51 bioptic mid zone amnion samples from premature babies. A total of 6352 proteins were identified. Bioinformatics analyses revealed a ranked core of 159 proteins maximizing the discrimination between the selected clinical stratification groups allowing to distinguish conditions of absent (FIR 0) from maximal Fetal Inflammatory Response (FIR 3) stratified in function of Maternal Inflammatory Response (MIR) grade. Matrix metallopeptidase-9 (MMP-9) was the top differentially expressed protein. Gene Ontology enrichment analysis of the core proteins showed significant changes in the biological pathways associated to inflammation and regulation of immune and infection response. Data suggest that the conditions determining PTB would be a transversal event, secondary to the maternal inflammatory response causing a breakdown in fetal-maternal tolerance, with fetal inflammation being more severe than maternal one. We also highlight matrix metallopeptidase-9 as a potential predictive biomarker of PTB that can be assayed in the maternal serum, for future investigation

A class of multidimensional IRT models for testing unidimensionality and clustering items

2PL model, EM algorithm, latent class model, NAEP data, Rasch model,

Contribution to the floristic knowledge of Lipari and Panarea Islands (Sicilia, Italy)

The inventory of the taxa collected in 2022 during the annual field trip of the Working Group for Floristics, Systematics and Evolution of the Italian Botanical Society is reported. The field trip was held from 19th to 22th April in the islands of Lipari and Panarea (Aeolian Islands, Sicilia). Overall, 1,664 herbarium specimens were deposited in public and private herbaria. The flora documented for the studied area amounts to 386 specific and subspecific taxa, belonging to 241 genera and 74 families. Centaurea aeolica, Helichrysum litoreum (Asteraceae), and Dianthus rupicola subsp. aeolicus (Caryophyllaceae) were the only three Italian endemics found in the study area, whereas 48 alien taxa were recorded. Dimorphotheca ecklonis (Asteraceae), Nassella tenuissima (Poaceae), Solanum torvum (Solanaceae), and Viola wittrockiana (Violaceae) are casual alien species new to Sicilia, whereas Oenothera odorata (Onagraceae) is a new naturalized alien species for the Italian vascular flora

Contribution to the floristic knowledge of Lipari and Panarea Islands (Sicilia, Italy)

The inventory of the taxa collected in 2022 during the annual field trip of the Working Group for Floristics, Systematics and Evolution of the Italian Botanical Society is reported. The field trip was held from 19th to 22th April in the islands of Lipari and Panarea (Aeolian Islands, Sicilia). Overall, 1,664 herbarium specimens were deposited in public and private herbaria. The flora documented for the studied area amounts to 386 specific and subspecific taxa, belonging to 241 genera and 74 families. Centaurea aeolica, Helichrysum litoreum (Asteraceae), and Dianthus rupicola subsp. aeolicus (Caryophyllaceae) were the only three Italian endemics found in the study area, whereas 48 alien taxa were recorded. Dimorphotheca ecklonis (Asteraceae), Nassella tenuissima (Poaceae), Solanum torvum (Solanaceae), and Viola wittrockiana (Violaceae) are casual alien species new to Sicilia, whereas Oenothera odorata (Onagraceae) is a new naturalized alien species for the Italian vascular flora

Mokken Scale Analysis for Dichotomous Items Using Marginal Models

marginal models, Mokken scale analysis, scalability coefficients, test construction,

Notulae to the Italian native vascular flora: 17

In this contribution, new data concerning the distribution of native vascular flora in Italy are presented. It includes new records, confirmations, and exclusions to the Italian administrative regions for taxa in the genera Anacyclus, Anthyllis, Bolboschoenus, Catapodium, Festuca, Hordeum, Lavandula, Ophrys, Pseudopodospermum, Rosa, Sorbus and Spergula. Nomenclatural and distribution updates, published elsewhere, and corrigenda are provided as Suppl. material 1