Air-sea interaction in the tropical Pacific Ocean

Charts of 3-month sea surface temperature (SST) anomalies in the eastern tropical Pacific Ocean were produced for the period 1949 to 1970. The anomalies along the United States and South American west coasts and in the eastern tropical Pacific appeared to be oscillating in phase during this period. Similarly, the satellite-derived cloudiness for each of four quadrants of the Pacific Ocean (130 deg E to 100 deg W, 30 deg N to 25 deg S) appeared to be oscillating in phase. In addition, a global tropical cloudiness oscillation from 30 deg N to 30 deg S was noted from 1965 to 1970, by using monthly satellite television nephanalyses. The SST anomalies were found to have a good degree of correlation both positive and negative with the following monthly geophysical parameters: (1) satellite-derived cloudiness, (2) strength of the North and South Pacific semipermanent anticyclones, (3) tropical Pacific island rainfall, and (4) Darwin surface pressure. Several strong direct local and crossequatorial relationships were noted. In particular, the high degree of correlation between the tropical island rainfall and the SST anomalies (r = +0.93) permitted the derivation of SST's for the tropical Pacific back to 1905. The close occurrence of cold tropical SST and North Pacific 700-mb positive height anomalies with central United States drought conditions was noted

Identification of Replication Competent Murine Gammaretroviruses in Commonly Used Prostate Cancer Cell Lines

A newly discovered gammaretrovirus, termed XMRV, was recently reported to be present in the prostate cancer cell line CWR22Rv1. Using a combination of both immunohistochemistry with broadly-reactive murine leukemia virus (MLV) anti-sera and PCR, we determined if additional prostate cancer or other cell lines contain XMRV or MLV-related viruses. Our study included a total of 72 cell lines, which included 58 of the 60 human cancer cell lines used in anticancer drug screens and maintained at the NCI-Frederick (NCI-60). We have identified gammaretroviruses in two additional prostate cancer cell lines: LAPC4 and VCaP, and show that these viruses are replication competent. Viral genome sequencing identified the virus in LAPC4 and VCaP as nearly identical to another known xenotropic MLV, Bxv-1. We also identified a gammaretrovirus in the non-small-cell lung carcinoma cell line EKVX. Prostate cancer cell lines appear to have a propensity for infection with murine gammaretroviruses, and we propose that this may be in part due to cell line establishment by xenograft passage in immunocompromised mice. It is unclear if infection with these viruses is necessary for cell line establishment, or what confounding role they may play in experiments performed with these commonly used lines. Importantly, our results suggest a need for regular screening of cancer cell lines for retroviral “contamination”, much like routine mycoplasma testing