A novel interdigitated capacitor based biosensor for detection of cardiovascular risk marker

C-reactive protein (CRP) is a potential biomarker whose elevated levels in humans determine cardiovascular disease risk and inflammation. In this study, we have developed a novel capacitive biosensor for detection of CRP-antigen using capacitor with interdigitated gold (GID) electrodes on nanocrystalline diamond (NCD) surface. The NCD surface served as a dielectric layer between the gold electrodes. GID-surface was functionalized by antibodies and the immobilization was confirmed by Fourier transform spectroscopy (FT-IR) and contact angle measurements. The CRP-antigen detection was performed by capacitive/dielectric-constant measurements. The relaxation time and polarizability constants were estimated using Cole-Cole model. Our results showed that the relaxation time constant (tau) of only CRP-antibody was within 10(-16)-10(-13) s, which was increased to 10(-11) s after the incubation with CRP-antigen, suggesting that the CRP-antigen was captured by the antibodies on GID-surface. In addition, polarizability constant(m) of CRP was also increased upon incubation with increasing concentration of CRP-antigen. Our results showed that the response of GID-NCD-based capacitive biosensor for CRP-antigen was dependent on both concentration (25-800 ng/ml) as well as frequency (50-350 MHz). Furthermore, using optimized conditions, the GID-NCD based capacitive biosensor developed in this study can potentially be used for detection of elevated levels of protein risk markers in suspected subjects for early diagnosis of disease

Review on carbon-derived, solid-state, micro and nano sensors for electrochemical sensing applications

The aim of this review is to summarize the most relevant contributions in the development of electrochemical sensors based on carbon materials in the recent years. There have been increasing numbers of reports on the first application of carbon derived materials for the preparation of an electrochemical sensor. These include carbon nanotubes, diamond like carbon films and diamond film-based sensors demonstrating that the particular structure of these carbon material and their unique properties make them a very attractive material for the design of electrochemical biosensors and gas sensors. Carbon nanotubes (CNT) have become one of the most extensively studied nanostructures because of their unique properties. CNT can enhance the electrochemical reactivity of important biomolecules and can promote the electron-transfer reactions of proteins (including those where the redox center is embedded deep within the glycoprotein shell). In addition to enhanced electrochemical reactivity, CNT-modified electrodes have been shown useful to be coated with biomolecules (e.g., nucleic acids) and to alleviate surface fouling effects (such as those involved in the NADH oxidation process). The remarkable sensitivity of CNT conductivity with the surface adsorbates permits the use of CNT as highly sensitive nanoscale sensors. These properties make CNT extremely attractive for a wide range of electrochemical sensors ranging from amperometric enzyme electrodes to DNA hybridization biosensors. Recently, a CNT sensor based fast diagnosis method using non-treated blood assay has been developed for specific detection of hepatitis B virus (HBV) (human liver diseases, such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma caused by hepatitis B virus). The linear detection limits for HBV plasma is in the range 0.5–3.0 μL−1 and for anti- HBVs 0.035–0.242 mg/mL in a 0.1 M NH4H2PO4 electrolyte solution. These detection limits enables early detection of HBV infection in suspected serum samples. Therefore, non-treated blood serum can be directly applied for real-time sensitive detection in medical diagnosis as well as in direct in vivo monitoring. Synthetic diamond has been recognized as an extremely attractive material for both (bio-) chemical sensing and as an interface to biological systems. Synthetic diamond have outstanding electrochemical properties, superior chemical inertness and biocompatibility. Recent advances in the synthesis of highly conducting nanocrystalline-diamond thin films and nano wires have lead to an entirely new class of electrochemical biosensors and bio-inorganic interfaces. In addition, it also combines with development of new chemical approaches to covalently attach biomolecules on the diamond surface also contributed to the advancement of diamond-based biosensors. The feasibility of a capacitive field-effect EDIS (electrolyte-diamond-insulatorsemiconductor) platform for multi-parameter sensing is demonstrated with an O-terminated nanocrystalline-diamond (NCD) film as transducer material for the detection of pH and penicillin concentration. This has also been extended for the label-free electrical monitoring of adsorption and binding of charged macromolecules. One more recent study demonstrated a novel bio-sensing platform, which is introduced by combination of a) geometrically controlled DNA bonding using vertically aligned diamond nano-wires and b) the superior electrochemical sensing properties of diamond as transducer material. Diamond nanowires can be a new approach towards next generation electrochemical gene sensor platforms. This review highlights the advantages of these carbon materials to promote different electron transfer reactions specially those related to biomolecules. Different strategies have been applied for constructing carbon material-based electrochemical sensors, their analytical performance and future prospects are discussed

LL-valley electron gg factor in bulk GaAs and AlAs

We study the Land\'e $g$-factor of conduction electrons in the $L$-valley of bulk GaAs and AlAs by using a three-band $\mathbf{k}\cdot\mathbf{p}$ model together with the tight-binding model. We find that the $L$-valley $g$-factor is highly anisotropic, and can be characterized by two components, $g_{\perp}$ and $g_{\|}$. $g_{\perp}$ is close to the free electron Land\'e factor but $g_{\|}$ is strongly affected by the remote bands. The contribution from remote bands on $g_{\|}$ depends on how the remote bands are treated. However, when the magnetic field is in the Voigt configuration, which is widely used in the experiments, different models give almost identical $g$-factor.Comment: 4 pages, 1 figure, To be published in J. App. Phys. 104, 200

Nanocrystalline diamond film for biosensor applications

In this study, we have developed a novel capacitive biosensor based oil interdigitated gold nanodiamond (GID-NCD) electrode for detection of C-reactive protein (CRP) antigen. CRP is one of the plasma proteins known as acute-phase proteins and its levels rise dramatically during inflammatory processes occurring in the body. It has been reported that CRP in serum can be used for risk assessment of cardiovascular diseases. The antibodies immobilization were confirmed by Fourier transform spectroscopy (FTIR) and contact angle measurements. In this capacitive biosensor, nanocrystalline diamond acting as a dielectric layer between the electrodes. The CRP antigen detection was performed by capacitive/dielectric-constant measurements. Our results showed that the response of NCD-based capacitive-based biosensor for CRP antigen was dependent on both concentration (25-800 ng/ml) as well as frequency (50-350 MHz). Furthermore, using optimized conditions, the biosensors developed in this study can be potentially used for detection of elevated level of risk markers protein in suspected subjects for early diagnosis of disease

Topological Gauge Structure and Phase Diagram for Weakly Doped Antiferromagnets

We show that the topological gauge structure in the phase string theory of the {\rm t-J} model gives rise to a global phase diagram of antiferromagnetic (AF) and superconducting (SC) phases in a weakly doped regime. Dual confinement and deconfinement of holons and spinons play essential roles here, with a quantum critical point at a doping concentration $x_c\simeq 0.043$. The complex experimental phase diagram at low doping is well described within such a framework.Comment: 4 pages, 2 figures, modified version, to appear in Phys. Rev. Let

Optical spectroscopy study of Nd(O,F)BiS2 single crystals

We present an optical spectroscopy study on F-substituted NdOBiS$_2$ superconducting single crystals grown using KCl/LiCl flux method. The measurement reveals a simple metallic response with a relatively low screened plasma edge near 5000 \cm. The plasma frequency is estimated to be 2.1 eV, which is much smaller than the value expected from the first-principles calculations for an electron doping level of x=0.5, but very close to the value based on a doping level of 7$\%$ of itinerant electrons per Bi site as determined by ARPES experiment. The energy scales of the interband transitions are also well reproduced by the first-principles calculations. The results suggest an absence of correlation effect in the compound, which essentially rules out the exotic pairing mechanism for superconductivity or scenario based on the strong electronic correlation effect. The study also reveals that the system is far from a CDW instability as being widely discussed for a doping level of x=0.5.Comment: 5 pages, 5 figure

Entanglement renormalization of anisotropic XY model

The renormalization group flows of the one-dimensional anisotropic XY model and quantum Ising model under a transverse field are obtained by different multiscale entanglement renormalization ansatz schemes. It is shown that the optimized disentangler removes the short-range entanglement by rotating the system in the parameter space spanned by the anisotropy and the magnetic field. It is understood from the study that the disentangler reduces the entanglement by mapping the system to another one in the same universality class but with smaller short range entanglement. The phase boundary and corresponding critical exponents are calculated using different schemes with different block sizes, look-ahead steps and truncation dimensions. It is shown that larger truncation dimension leads to more accurate results and that using larger block size or look-ahead step improve the overall calculation consistency.Comment: 5 pages, 3 figure

Nitrogen dynamics in the shallow groundwater of a riparian wetland zone of the Garonne, SW France: nitrate inputs, bacterial densities, organic matter supply and denitrification measurements

This study highlights the role of interactions between surface and sub-surface water of the riparian zone of a large river (the Garonne, SW France). Information is given about the role of surface water in supplying Dissolved Organic Carbon (DOC ) to the riparian zone for nitrate removal processes. The densities of bacteria (up to 3.3106 cell m L-1) in groundwater are strongly conditioned by the water moving during flood events. Total bacterial densities in groundwater were related to surface water bacterial densities. In sediment, total bacteria are attached mainly to fine particles (90 % in the fraction < 1 mm). Spatial variations in organic carbon and nitrate content in groundwater at the site studied are correlated with exchanges between the groundwater and the river, from the upstream to the downstream part of the meander. Total bacterial densities, nitrate and decressing organic carbon concentrations follow the same pattern. These results suggest that, in this kind of riparian wetland, nitrate from alluvial groundwater influenced by agricultural practices may be denitrified by bacteria in the presence of organic carbon from river surface water

Evolution of Ego-networks in Social Media with Link Recommendations

Ego-networks are fundamental structures in social graphs, yet the process of their evolution is still widely unexplored. In an online context, a key question is how link recommender systems may skew the growth of these networks, possibly restraining diversity. To shed light on this matter, we analyze the complete temporal evolution of 170M ego-networks extracted from Flickr and Tumblr, comparing links that are created spontaneously with those that have been algorithmically recommended. We find that the evolution of ego-networks is bursty, community-driven, and characterized by subsequent phases of explosive diameter increase, slight shrinking, and stabilization. Recommendations favor popular and well-connected nodes, limiting the diameter expansion. With a matching experiment aimed at detecting causal relationships from observational data, we find that the bias introduced by the recommendations fosters global diversity in the process of neighbor selection. Last, with two link prediction experiments, we show how insights from our analysis can be used to improve the effectiveness of social recommender systems.Comment: Proceedings of the 10th ACM International Conference on Web Search and Data Mining (WSDM 2017), Cambridge, UK. 10 pages, 16 figures, 1 tabl

Mean-Field Description of Phase String Effect in the t−Jt-J Model

A mean-field treatment of the phase string effect in the $t-J$ model is presented. Such a theory is able to unite the antiferromagnetic (AF) phase at half-filling and metallic phase at finite doping within a single theoretical framework. We find that the low-temperature occurrence of the AF long range ordering (AFLRO) at half-filling and superconducting condensation in metallic phase are all due to Bose condensations of spinons and holons, respectively, on the top of a spin background described by bosonic resonating-valence-bond (RVB) pairing. The fact that both spinon and holon here are bosonic objects, as the result of the phase string effect, represents a crucial difference from the conventional slave-boson and slave-fermion approaches. This theory also allows an underdoped metallic regime where the Bose condensation of spinons can still exist. Even though the AFLRO is gone here, such a regime corresponds to a microscopic charge inhomogeneity with short-ranged spin ordering. We discuss some characteristic experimental consequences for those different metallic regimes. A perspective on broader issues based on the phase string theory is also discussed.Comment: 18 pages, five figure