Nanoporous Structure of Bone Matrix at Osteoporosis from Data of Atomic Force Microscopy and IR Spectroscopy

It was found that in an osteoporotic bone the fraction of nanosized pores decreases, the mineral phase amorphizes, hydrated shells around mineralized particles of the bone matrix thicken, and adhesion forces increase. This contributes to the formation of water clusters similar to bulk water clusters compared to the healthy bone tissue and leads to the accumulation of more viscous liquid with increased intermolecular interaction forces in the pores of the bone matrix. Given this, the rates of chemical reactions proceeding in the water phase of ultrathin channels of general parts of collagen fibrils decrease. Ultimately, nanopores of collagen-apatite interfaces lose, to a certain extent, the capability of catalyzing the hydroxyapatite crystallization

Retrieval of Total Scattering Cross Sections of Molecules from Inhomogeneously Broadened Absorption Lines

A new method of retrieving quantitative information on the hard, soft, and diffraction collision’s frequencies from inhomogeneously pressure-broadened line profiles was proposed and tested. The essence of the method lies in the processing of recorded profiles using various profiles’ models containing these frequencies treated as adjustable parameters in different manners. Three self-broadened H2O absorption lines free of an instrumental function were processed with the use of four model line profiles. Retrieved set of pressure line broadening and narrowing constants allows finding out the sought frequencies. The estimation of the total scattering cross section, ∼630 Å2, in pure water vapor was made

THE WATER VAPOR LINESHIFT INVESTIGATIONS AT 5μm5\mu m REGION

1. P.W. Anderson, Phys. Rev. 76, 647-661 (1949) 2. M.R. Cherkasov, Optika i Spektroskopiya 40, 7-13 (1976)Author Institution: "CNRS, UPMC", Tour 13, 4 Place Jussieu, 75005 PARIS - FRANCE.; Institute of Atmospheric Optics, Tomsk, 634055 - RUSSIA.Water vapor lineshifts induced by nitrogen pressure have been measured using FT-spectrometer with multi-pass absorption cell at $0.0025 cm^{-1}$ resolution in $1850 - 2230 cm^{-1}$ spectral region. The measurements have been carried out from three spectra with 32 meters absorption path; the $H_{2}O$ pressures being equal to 2, 6 and 5 torr with nitrogen filling equal respectively to 123 and 201 torr for the last two spectra. A special procedure have been used to minimize the errors connected with the assymetry of the contours because of apparatus effects. The fitting of Voigt contour parameters was used to obtain lineshift parameters. Accuracy of lineshift coefficient determination was better than 1 mk/atm. The lineshift coefficients have been determined for well resolved isolated lines of R-branch of $\nu_{2}$ band with high quantum numbers J up to 18. Their values change from +14.4 to -14.5 mk/atm. The lineshift coefficients have been calculated using the Anderson method and cut-of-free procedure. The dipole-quadrupole, quadrupole-quadrupole, dipole-hexadecapole and polarizational interactions were included in the calculations. The calculations use the fitted parameter - the mean polarizability of the molecule in the (010) vibrational state. The calculated lineshift coefficients agree satisfactory with the measured ones. The contributions of different type of intermolecular potentials and their dependences on J value will be discussed. The research described in this publication was made possible in part by grant N. NY3000 from the International Science Foundation