STUDING THE PRECONDITIONS OF ESTABLISHING THE LEGISLATION OF RUSSIA IN THE FIELD OF RENEWABLE ENERGY

The initial stage of development of the legal framework affecting the area of renewable energy discusses in details in this paper. The author analyzes the prerequisites of becoming law in the direction of non-traditional and renewable energy. The legislative documents, which were adopted prior to the amendment of November 4, 2007 the Federal Law "On Electric Power Industry" were studied in details, and we presented, how the legislative documents could affect the development of legislation in this area.В работе подробно рассмотрен первоначальный этап развития законодательной базы, затрагивающей область применения возобновляемых источников энергии. Автором были проанализированы предпосылки становления законодательства в направлении нетрадиционной и возобновляемой энергетики. В данной работе подробно изучены законодательные документы, которые были приняты в период до внесения поправок 4 ноября 2007 года в Федеральный закон «Об электроэнергетике» и представлено, как законодательные документы могли повлиять на развитие законодательства в рассматриваемой сфере

Ca2+-Transport through Plasma Membrane as a Test of Auxin Sensitivity

Auxin is one of the crucial regulators of plant growth and development. The discovered auxin cytosolic receptor (TIR1) is not involved in the perception of the hormone signal at the plasma membrane. Instead, another receptor, related to the ABP1, auxin binding protein1, is supposed to be responsible for the perception at the plasma membrane. One of the fast and sensitive auxin-induced reactions is an increase of Ca2+ cytosolic concentration, which is suggested to be dependent on the activation of Ca2+ influx through the plasma membrane. This investigation was carried out with a plasmalemma enriched vesicle fraction, obtained from etiolated maize coleoptiles. The magnitude of Ca2+ efflux through the membrane vesicles was estimated according to the shift of potential dependent fluorescent dye diS-C3-(5). The obtained results showed that during coleoptiles ageing (3rd, 4th and 5th days of seedling etiolated growth) the magnitude of Ca2+ efflux from inside-out vesicles was decreased. Addition of ABP1 led to a recovery of Ca2+ efflux to the level of the youngest and most sensitive cells. Moreover, the efflux was more sensitive, responding from 10−8 to 10−6 M 1-NAA, in vesicles containing ABP1, whereas native vesicles showed the highest efflux at 10−6 M 1-NAA. We suggest that auxin increases plasma membrane permeability to Ca2+ and that ABP1 is involved in modulation of this reaction