Phytostabilization of Pb and Cd polluted soils using Helianthus petiolaris as pioneer aromatic plant species

The area of soils polluted with heavy metals is increasing due to industrialization and globalization. Aromatic plant species can be a suitable alternative way for agricultural valorization and phytomanagement of such soils by the commercialization of essential oils avoiding risks for the food chain. The potential of growing Helianthus petiolaris in heavy metal polluted soils was assessed in pot experiments using spiked soils and soils from a shooting range. In terms of phytostabilization, H. petiolaris could grow in soils containing 1000 mg/kg Pb2þ, 50 mg/kg Cd2þ, accumulating more than three times the soil Cd content in the aerial parts and translocating significant amounts of Pb to the aerial parts when growing in soils polluted with up to 500 mg/kg Pb. When phytostabilization is considered, phytotoxicity of heavy metals strongly depends on the rhizospheric microbial communities, either by mitigating trace element phytotoxicity or promoting plant growth via phytohormone production. So, the effects of heavy metals on the diversity of the rhizospheric bacterial community were assessed using DNA-fingerprinting.Fil: Saran, Anabel. Instituto Nacional de Tecnología Agropecuaria; Argentina. Hasselt University; BélgicaFil: Fernandez, L.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Cora, F.. Universidad Nacional de La Pampa; ArgentinaFil: Savio, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Thijs, S.. Hasselt University; BélgicaFil: Vangronsveld, J.. Hasselt University; Bélgica. Maria Curie-Sklodowska; PoloniaFil: Merini, Luciano Jose. Instituto Nacional de Tecnología Agropecuaria; Argentina. Gobierno de la Provincia de la Pampa.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Bioaugmentation with PGP-trace element tolerant bacterial consortia affects Pb uptake by Helianthus annuus grown on trace element polluted military soils

In this study, we sought to compose consortia of plant growth-promoting (PGP) and trace element tolerant bacteria, to improve plant growth and inhibit uptake and translocation of trace elements, eventually allowing the cultivation of profitmaking crops on trace elements polluted soils, reducing the risks of entrance of these elements into the food chain. Sunflower (Helianthus annuus L.) was grown on two polluted military soils (MS1 and MS2) in greenhouse microcosms and inoculated with three different bacterial consortia (C1, C2, C3). Growth and physiological status of the plants were unaffected during the experiment with the inoculation. After 2months, plants were harvested. Consortium C2 and C3 decreased Pb shoot bioaccumulation by respectively 80–85% when plants were grown in the MS1 and even to concentrations below detection limit in plants grown in MS2. Differences in uptake and (sub)cellular localization of Pb and Cd in selected bacterial isolates were investigated in vitro by TEM-EDX. Pb absorption was observed by Bacillus wiedmanni ST29 and Bacillus paramycoides ST9 cultures. While adsorption at the bacterial cell wall was observed by Bacillus paramycoides ST9 and retention in the extracellular matrix by Cellulosimicrobium cellulans ST54.Fil: Saran, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Hasselt University; BélgicaFil: Imperato, V.. Hasselt University; BélgicaFil: Fernandez, L.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; ArgentinaFil: Vannucchi, F.. Scuola Superiore Sant’Anna; ItaliaFil: Steffanie, N. M.. Hasselt University; BélgicaFil: d'Haen, J.. Hasselt University; BélgicaFil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; ArgentinaFil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie Sklodowska University; PoloniaFil: Thijs, Sofie. Hasselt University; Bélgic

Additional file 1: of Potential risk factors for zoonotic disease transmission among Mongolian herder households caring for horses and camels

Household Survey. (DOCX 55 kb