RECONSTRUCTION OF HYPOPHYSIAL-THYROID LINK OF REGULATION OF REPRODUCTIVE SYSTEM IN WOMEN WITH CHRONIC VIRAL HEPATITIS

The article presents the results of a study of levels of hormones of hypophysial-thyroid, system, in women with parenteral chronic viral hepatitis B and C in the light menstrua! disorders. We examined. 77 women of reproductive age: 42 patients with, chronic viral hepatitis and 35 healthy controls. We determined, the concentration of thyroid-stimulating hormone, triiodothyronine, thyroxine, free triiodothyronine and. free thyroxine. We identified. some differences in the concentrations of the studied, hormones in patients with, viral hepatitis depending on the nature of the menstrual cycle

Assessment of functional state of the pituitary-gonadal system in fertile women with acute hepatitis A

Liver is a necessary participant of the metabolism of steroid hormones, so dysfunction of pituitary-gonadal system is possible at viral hepatitis A. We assessed functional state of pituitary-gonadal part of neuroendocrinal regulation system in fertile women with acute hepatitis A and detected changes that can be regarded as compensatory-adaptive shielding of an organism

Modern concepts of viral hepatitis (review of literature)

We conducted an analysis of Russian and foreign literature and an analysis of our researches on the problem of etiology and pathogenesis of viral hepatitis was showed. Viral hepatitis occupies a leading position among infectious disease. Chronic forms of viral hepatitis B and C is the most prevalent. Among them more than 60 % are registered in age from 20 to 39 years. In spite of the fact that pathogenesis, clinical aspects and chronization of these forms are different, they have the general processes in a liver. The changes of these processes of the general systems of an organism (such as the neuroendocrine regulation, lipid status and lipid peroxidation - antioxidant system) have the particular importance. Studying these variables under abnormalities of reproductive health in women with hepatitis is particularly topical

ФАКТОРЫ ВЗАИМНОГО ОТЯГОЩЕНИЯ МНОЖЕСТВЕННЫХ ХРОНИЧЕСКИХ ОЧАГОВ ИНФЕКЦИИ И ГЕНЕРАЛИЗОВАННОГО ПАРОДОНТИТА

Introduction. The Inflammatory Diseases of the Parodont (IDP) of generalized character are closely connected petrogenetically with internal pathology and are a serious factor of its burdening, forming a negative comorbidity. Pro-inflammatory reactions and metabolic shifts, such as oxidizing stress, violation of regulatory participation of the vital minerals at an imbalance of their distribution in biological environments are the inflammatory mechanisms uniting IDP with chronic inflammatory diseases of visceral of different localization. The objective of the study was identification of metabolic factors of pathogenetic community of GIDP and a comorbid chronic focal infection of different localization. Material and methods. The activity of systemic factors of inflammation were studied in the patients with generalized periodontitis (GP) of developed stage on a background of multiple chronic foci of infection: level and ratios of pro- and antiinflammatory cytokines – interleukins 2,6,10, TNF-α, C – reactive protein in comparison with the activity of oxidation of lipids, proteins, low molecular weight thiols, assessment of levels of Zn, Cu, Fe in biological environments and the hepsidin blood level. Results and discussion. Significantly more expressed metabolic disorders in comorbidity multiple chronic foci of infection and GP, than in the absence of periodontium lesions have been shown. Increased lipid peroxidation and free radical oxidation of thiols (p<0.01), increased levels of C – reactive protein level and pro-inflammatory cytokines (TNF-α, IL 6), and imbalance in the distribution of such trace elements as copper, zinc, iron in environments of organism were found in patients with combined pathology of comorbidity multiple chronic foci of infection and GP. Conclusions. Thus, the findings of the study demonstrate that formation of multiple chronic foci of infection in an organism is connected with increase of mediators of system inflammation in blood, disorders of redox metabolism and deficiency of such trace elements as Cu, Zn, Fe which are the key factors of a enzyme link of antioxidant protection.Введение. Воспалительные заболевания пародонта (ВЗП) генерализованного характера патогенетически тесно связаны с внутренней патологией и являются серьезным фактором ее отягощения, формируя негативную коморбидность. Воспалительными механизмами, объединяющими ВЗП с хроническими воспалительными заболеваниями внутренних органов различной локализации, являются провоспалительные реакции и метаболические сдвиги, такие как окислительный стресс, нарушение регуляторного участия жизненно важных микроэлементов при дисбалансе распределения их в биологических средах. Целью исследования явилась идентификация метаболических факторов патогенетической общности ГВЗП и коморбидной хронической очаговой инфекции разной локализации. Материал и методы. У больных генерализованным пародонтитом (ГП) развившейся стадии на фоне множественных хронических очагов инфекции (МХОИ) изучена активность системно-действующих факторов воспаления: уровень и соотношения про- и противовоспалительных цитокинов – интерлейкинов-2, 6, 10, TNF-α, С-реактивного белка – в сопоставлении с активностью окисления липидов, белков, низкомолекулярных тиолов, оценкой уровней Zn, Cu, Fe в биологических средах и гепсидина в крови. Результаты исследования. Показаны достоверно более выраженные метаболические нарушения при коморбидности МХОИ и ГП, чем при отсутствии признаков поражения пародонта. У больных с сочетанной патологией МХОИ и ГП было установлено повышение активности перекисного окисления липидов и свободно-радикального окисления тиолов (р<0,01), повышение уровня СРБ и провоспалительных цитокинов (TNF-α, IL-6) и разбалансировка распре- деления микроэлементов, таких как медь, цинк, железо в биосредах организма. Заключение. Таким образом, полученные данные свидетельствуют о том, что формирование множественных хронических очагов инфекции в организме связано с нарастанием в крови медиаторов системного воспаления, нарушениями окислительно-восстановительного метаболизма и дефицитом таких микроэлементов, как Cu, Zn, Fe – ключевых факторов ферментного звена антиоксидантной защиты. Ключевые слова: очаговая инфекция, генерализованный пародонтит, воспаление>< 0,01), повышение уровня СРБ и провоспалительных цитокинов (TNF-α, IL-6) и разбалансировка распределения микроэлементов, таких как медь, цинк, железо в биосредах организма. Заключение. Таким образом, полученные данные свидетельствуют о том, что формирование множественных хронических очагов инфекции в организме связано с нарастанием в крови медиаторов системного воспаления, нарушениями окислительно-восстановительного метаболизма и дефицитом таких микроэлементов, как Cu, Zn, Fe – ключевых факторов ферментного звена антиоксидантной защиты.

Use of Gd-doped ZnO surfaces to increase fluorescence of globular proteins

Novel ZnO - Gd3+ materials were prepared by sol-gel method and photoluminescence properties of their films were studied for biomedical application. Biosensor’s properties ZnO - Gd3+ films were investigated under the action of rabbit immunoglobulin G (IgG) in concentrations 10-9, 10-11 and 10-13 M in solution depositing on the surface of film. The doping of ZnO film by Gd3+ in an amount from 1-12 mass.% with increments of 1 mass. % results in significant variations of fluorescence spectra ZnO - Gd3+ films. The ZnO substrate without of Gd3+ compounds allows detecting IgG in a concentration of 10-11 M by fluorescence spectroscopy method by registration in UV region (320-450 nm). By using ZnO - Gd3+ system it was obtained the increase the concentration limit of immunoglobulin (10-13M) detection. In the only case (the content of Gd3+ in ZnO film - 8 mass. %) was observed batochromic shift of fluorescence band maximum by 20 nm (from 364 to 385nm) when interacting with immunoglobulin regardless of the concentration of the last. © 2018 TANGER Ltd. All Rights Reserved

Optical properties of detonation nanodiamond complexes with globular proteins in water solution

It has been studied the changing of spectral characteristics of water-soluble 10 nm detonation nanodiamonds (DND) upon their complexation with a globular blood protein: bovine hemoglobin (Hb), human serum albumin (HSA) and rabbit immunoglobulin G (IgG) by fluorescence and absorption spectroscopy. The structure of the protein globule and its concentration in aqueous solution can significantly affect the complexation with DND (2·10-3 g/l) in the process of ultrasonic treatment (42 kHz). In all cases, it was observed when adding the DND the increasing of intensity of own luminescence of proteins (em = 330-346 nm) and bathochromic shift of protein luminescence bands. The magnitude of the shift of the maximum of protein luminescence bands at a protein concentration of 10-11 M was 5, 16, 18 nm, at the concentration 10-5 M - 7, 9, 2 nm for Hb, HSA and IgG, respectively. The aggregates’ size of DND was evaluated by AFM method in monocomponent film on the glass substrate. The results are of interest for investigations of the dynamic interaction of DND with biomacromolecules in vivo. © 2018 TANGER Ltd. All Rights Reserved

Dependence of Gd-doped ZnO surfaces fluorescent properties on dopant concentration

Substrates containing zinc oxide and different amounts of gadolinium on the surface of the zinc oxide nano particles for biosensorics were obtained for the first time by the standard sol-gel technique. This is a new material has not yet been extensively studied. In this paper, we present the results of the photoluminescent response of the zinc oxide surface when it is modified by various amounts of gadolinium during the classical sol-gel method of the surface obtaining. Aspects of creating a modified surface of zinc oxide are highlighted. The used concentrations of gadolinium vary from 1 to 33% caused both quenching and ignition of photoluminescence at the wavelength of 356 nm upon excitation into the absorption band of protein 280 nm. The photoluminescence intensity of zinc oxide without doping is 40 relative value units, the addition of 8% Gd increases the photoluminescence by 7 times (280 r.u.), the addition of 31% Gd decreases the photoluminescence by 40 times compared to undoped zinc oxide. The resulting possibility of increased luminescence when the doping of the films with gadolinium willing, apparently, to increase the ratio “signal/background”, and therefore increase the sensitivity of the biosensor with fluorescent registration. © 2018 TANGER Ltd. All Rights Reserved

Use of Gd-doped ZnO surfaces to increase fluorescence of globular proteins

Novel ZnO - Gd3+ materials were prepared by sol-gel method and photoluminescence properties of their films were studied for biomedical application. Biosensor’s properties ZnO - Gd3+ films were investigated under the action of rabbit immunoglobulin G (IgG) in concentrations 10-9, 10-11 and 10-13 M in solution depositing on the surface of film. The doping of ZnO film by Gd3+ in an amount from 1-12 mass.% with increments of 1 mass. % results in significant variations of fluorescence spectra ZnO - Gd3+ films. The ZnO substrate without of Gd3+ compounds allows detecting IgG in a concentration of 10-11 M by fluorescence spectroscopy method by registration in UV region (320-450 nm). By using ZnO - Gd3+ system it was obtained the increase the concentration limit of immunoglobulin (10-13M) detection. In the only case (the content of Gd3+ in ZnO film - 8 mass. %) was observed batochromic shift of fluorescence band maximum by 20 nm (from 364 to 385nm) when interacting with immunoglobulin regardless of the concentration of the last. © 2018 TANGER Ltd. All Rights Reserved

Dependence of Gd-doped ZnO surfaces fluorescent properties on dopant concentration

Substrates containing zinc oxide and different amounts of gadolinium on the surface of the zinc oxide nano particles for biosensorics were obtained for the first time by the standard sol-gel technique. This is a new material has not yet been extensively studied. In this paper, we present the results of the photoluminescent response of the zinc oxide surface when it is modified by various amounts of gadolinium during the classical sol-gel method of the surface obtaining. Aspects of creating a modified surface of zinc oxide are highlighted. The used concentrations of gadolinium vary from 1 to 33% caused both quenching and ignition of photoluminescence at the wavelength of 356 nm upon excitation into the absorption band of protein 280 nm. The photoluminescence intensity of zinc oxide without doping is 40 relative value units, the addition of 8% Gd increases the photoluminescence by 7 times (280 r.u.), the addition of 31% Gd decreases the photoluminescence by 40 times compared to undoped zinc oxide. The resulting possibility of increased luminescence when the doping of the films with gadolinium willing, apparently, to increase the ratio “signal/background”, and therefore increase the sensitivity of the biosensor with fluorescent registration. © 2018 TANGER Ltd. All Rights Reserved