1,283 research outputs found
Effect of heat and power extraction on turbojet-engine performance III : analytical determination of effects of shaft-power extraction
Effect of heat and power extraction on turbojet-engine performance I : analytical method of performance evaluation with compressor-outlet air bleed
Venus surface roughness and Magellan stereo data
Presented are results of some studies to develop tools useful for the analysis of Venus surface shape and its roughness. Actual work was focused on Maxwell Montes. The analyses employ data acquired by means of NASA's Magellan satellite. The work is primarily concerned with deriving measurements of the Venusian surface using Magellan stereo SAR. Roughness was considered by means of a theoretical analyses based on digital elevation models (DEM's), on single Magellan radar images combined with radiometer data, and on the use of multiple overlapping Magellan radar images from cycles 1, 2, and 3, again combined with collateral radiometer data
Using RNA Interference to Determine the Role of Varisin in the Innate Immune System of the Hard Tick Dermacentor variabilis (Acari: Ixodidae).
Defensins are an important component of the innate immune system of ticks. These small peptides are produced by various genera of ticks, and expressed in various tissues. In this study we used RNA interference to silence the expression of the defensin varisin produced by the hemocytes of the American dog tick, Dermacentor variabilis. Ticks were injected with double stranded varisin RNA prior to being placed on a rabbit. After feeding, the ticks were removed, bled, and the hemolymph plasma and hemocytes separated. Hemocytes were screened for the presence (or absence) of both varisin transcript and peptide. Varisin peptide was below detectable levels and the transcript showed a greater than 99% knockdown. The antimicrobial activity of the hemolymph plasma was reduced 2-4 fold compared to that of control injected ticks indicating varisin accounts for a large portion of the antimicrobial activity of the hemolymph
Recent results from the canfranc dark matter search with germanium detectors
Two germanium detectors are currently operating in the Canfranc Underground
Laboratory at 2450 m.w.e looking for WIMP dark matter. One is a 2 kg 76Ge IGEX
detector (RG-2) which has an energy threshold of 4 keV and a low-energy
background rate of about 0.3 c/keV/kg/day. The other is a small (234 g) natural
abundance Ge detector (COSME), of low energy threshold (2.5 keV) and an energy
resolution of 0.4 keV at 10 keV which is looking for WIMPs and for solar
axions. The analysis of 73 kg-days of data taken by COSME in a search for solar
axions via their photon Primakoff conversion and Bragg scattering in the Ge
crystal yields a 95% C.L. limit for the axion-photon coupling g < 2.8 10^-9
GeV^-1. These data, analyzed for WIMP searches provide an exclusion plot for
WIMP-nucleon spin-independent interaction which improves previous plots in the
low mass region. On the other hand, the exclusion plot derived from the 60
kg-days of data from the RG-2 IGEX detector improves the exclusion limits
derived from other ionization (non thermal) germanium detector experiments in
the region of WIMP masses from 30 to 100 GeV recently singled out by the
reported DAMA annual modulation effect.Comment: 6 pages, talk given at IDM2000, York, September 200
Influenza A Virus Hemagglutinin Antibody Escape Promotes Neuraminidase Antigenic Variation and Drug Resistance
Drugs inhibiting the influenza A virus (IAV) neuraminidase (NA) are the cornerstone of anti-IAV chemotherapy and prophylaxis in man. Drug-resistant mutations in NA arise frequently in human isolates, limiting the therapeutic application of NA inhibitors. Here, we show that antibody-driven antigenic variation in one domain of the H1 hemagglutinin Sa site leads to compensatory mutations in NA, resulting in NA antigenic variation and acquisition of drug resistance. These findings indicate that influenza A virus resistance to NA inhibitors can potentially arise from antibody driven HA escape, confounding analysis of influenza NA evolution in nature
Hepatitis C Virus Infection Causes Cell Cycle Arrest at the Level of Initiation of Mitosis
Chronic infection with the hepatitis C virus (HCV) is associated with increased risk for hepatocellular carcinoma (HCC). Chronic immune-mediated inflammation is likely to be an important factor in the development of HCV-associated HCC, but direct effects of HCV infection on the host cell cycle may also play a role. Although overexpression studies have revealed multiple interactions between HCV-encoded proteins and host cell cycle regulators and tumor suppressor proteins, the relevance of these observations to HCV-associated liver disease is not clear. We determined the net effect of these interactions on regulation of the cell cycle in the context of virus infection. Flow cytometry of HCV-infected carboxyfluorescein succinimidyl ester-labeled hepatoma cells indicated a slowdown in proliferation that correlated with abundance of viral antigen. A decrease in the proportions of infected cells in G1 and S phases with an accumulation of cells in G2/M phase was observed, compared to mock-infected controls. Dramatic decreases in markers of mitosis, such as phospho-histone H3, in infected cells suggested a block to mitotic entry. In common with findings described in the published literature, we observed caspase 3 activation, suggesting that cell cycle arrest is associated with apoptosis. Differences were observed in patterns of cell cycle disturbance and levels of apoptosis with different strains of HCV. However, the data suggest that cell cycle arrest at the interface of G2 and mitosis is a common feature of HCV infection
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