Diagnóstico de propriedades agrícolas no município de Cristalina-GO, visando estabelecer um sistema de produção integrada de feijão comum (Phaseolus vulgaris L.).

Está se conduzindo um projeto para subsidiar a implantação de um Modelo de Produção Integrada de Feijão. Realizou-se um diagnóstico dos sistemas de produção em uso e de algumas características das propriedades candidatas a sediarem essas Unidades. Esse diagnóstico norteou a elaboração do sistema de produção para feijão irrigado, que está sendo validado a campo com a participação dos produtores

Detection of copy number variations in nelore beef cattle with high-density SNP genotyping data.

Genome-wide single nucleotide polymorphism (SNP) genotyping platforms have been widely used in studies in diverse areas, ranging from population genetics to applied genetic improvement and breeding. SNP genotyping data generated with these platforms can also be used for detecting and genotyping copy number variations (CNVs). CNVs are defined as a variable copy numbers of DNA segments ranging from 50bp to several megabases (Mbp), in comparison with a reference genome. Several studies have identified an abundance of CNVs in human and domestic animal genomes, where it has been shown that they are involved in phenotypic variability. This initial study reports a high resolution map of CNVs in Nelore beef cattle generated with the PennCNV software. CNVs were called in a dataset from 1709 animals of the Nelore breed genotyped with Illumina BovineHD BeadChip for a total of 735,242 markers. After non-restrictive quality filtering, a total of 246,290 CNVs were identified on autosomal chromosomes, representing 219,997 and 26,293 gain and loss events, respectively. CNVs lengths ranged from 20.02 Kb to 8.37 Mb with an average of 352 Kb and a median of 204.5 Kb. The number of SNPs in each detected CNV varied from 20 to 2,116 with an average of 104 and a median of 63. A total of 138,066 CNVs were present in regions with annotated genes.P553

Looking for the "missing" Nelore genotypes.

SNP genotyping with High Density (HD) chips offers a relative low cost when compared to genotyping by re- sequencing using Next Generation Sequencing technologies, which is a significant advantage when a large number of individuals are under study. Currently, there are commercial HD chips for several economically important species, for very reasonable prices per sample

Genomic variant hotspots in nelore cattle revealed by missing genotypes.

In order to investigate whether or not the ?missing? genotypes actually reveal genomic variant hotspots, we examined BovineHD missing genotypes from a total of 1,709 Nelore DNA samples and used sequence data from eight of them to identify putative polymorphisms flanking the assayed SNPs.PAG 2015. Pôster P0289

Seleção de caprinos Moxotó para Núcleo de Conservação a partir de marcadores microssatélites.

Resumo: Foram testados 14 marcadores microssatélites em um estudo piloto para inferir a diversidade genética em 25 amostras de DNA genômico de caprinos da raça Moxotó provenientes de um rebanho particular e selecionar quais deveriam ser incorporados em um Núcleo de Conservação da mesma raça Moxotó. Os resultados comparados indicaram elevada heterozigosidade (He e Ho médios = 0,572), presença de alelos diagnósticos e relação genética entre as duas populações (FIS médio = 0,417). Esses são parâmetros desejáveis para candidatos a serem introduzidos em Núcleos com baixa diversidade, contudo, a alta variabilidade também pode indicar eventos de introgressão. Dessa forma, após uma análise de coordenadas principais, foi observado um agrupamento dos indivíduos em três diferentes grupos. Recalculou-se o coeficiente de co-ancestralidade e os alelos diagnósticos. Os resultados indicaram relação genética e presença de alelos diagnósticos exclusivos em dois desses grupos. Ao selecionar animais para Núcleos de Conservação é preciso levar em consideração as necessidades do Núcleo: aumento de variabilidade, manutenção do padrão da raça, evitar introdução de alelos não pertencentes à raça. Verificou-se, assim, que os marcadores microssatélites constituem ferramenta adequada para auxiliar essa seleção. [Selection of Moxotó goats for Conservation Nucleus from microsatellite markers]. Abstract: We tested 14 microsatellite markers in a pilot study to infer the genetic diversity in 25 samples of genomic DNA of Moxotó goats from a particular herd and to select which should be incorporated into a conservation nucleus of Moxotó goats. The results showed high heterozygosity (He and Ho mean = 0.572), presence of diagnostic alleles and genetic relationship between the two populations (FIS mean = 0.417). These parameters are interesting for candidates to be introduced in Nuclei with low diversity, however, the high variability may also indicate introgression events. Thus, after a principal coordinates analyses, were observed that individuals formed three different groups. The coefficients of co-ancestry and diagnostic alleles were recalculated. The results showed presence of genetic relationship and exclusive diagnostic alleles in two groups. When selecting animals we must take into account the needs of the Conservation Nucleus: increase the variability, maintenance of breed standard, avoidance the introduction of other races alleles. It was found thus that microsatellite markers are suitable tool to help this selectio

Genome Sequencing and de novo assembly of the South American Tiger Catfish (Pseudoplatystoma punctifer).

The South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were sequenced (2x160bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1.12pg = 1.095Gbp). Initial sequence assembly was performed with SOAPdenovo and generated 686.171 scaffolds spanning 1.41 Gbp (N50 scaffold length: 908Kbp, L50 scaffold count: 420). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species, which will be a valuable resource for basic biology studies, and marker detection/selection for use in genetic improvement and resource conservation activities.PAG 2016. Pôster P0481

BRS 328 - Double haploid bread wheat cultivar.

The wheat cultivar 'BRS 328' was developed by Embrapa from a cross between KleinH3394 and PF 990744. The pre-harvest sprouting resistance of 'BRS 328' is good and the grain yieldhigh. It is classified as bread wheat cultivar in all regions where it is recommended for cultivation

BRS: parrudo.

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