Phosphoproteins associated with cyclic nucleotide stimulation of ciliary motility in Paramecium

Permeabilized, MgATP-reactivated cells of Paramecium (models) respond to cyclic AMP and cyclic GMP by increasing forward swimming speed. In association with the motile response, cyclic AMP and 8-bromo-cyclic GMP (8-Br-cyclic GMP) stimulated protein phosphorylation. Cyclic AMP addition to permeabilized cells reproducibly stimulated the phosphorylation of 10 proteins, ranging in molecular weight from 15 to 110K (K = 10^3 M_r). 8-Br-cyclic GMP, which selectively activates the cyclic GMP-dependent protein kinase of Paramecium, stimulated the phosphorylation of a subset of the proteins phosphorylated by cyclic AMP. Ca^(2+) addition caused backward swimming and stimulated the phosphorylation of four substrates, including a 25K target that may also be phosphorylated in response to cyclic nucleotide addition. Ba^(2+) and Sr^(2+) also induced backward swimming, but did not cause detectable phosphorylation. To identify ciliary targets of cyclic nucleotide-dependent protein kinase activity, permeabilized cells were deciliated following reactivation of motility with Mg-[y-^(32)P]ATP in the presence or absence of cyclic nucleotide. Soluble proteins of the deciliation supernatant were enriched in 15 cyclic AMP-stimulated phosphoproteins, ranging in molecular weight from 15 to 95K. Most of the ciliary substrates were axonemal and could be released by high salt solution. A 29K protein that copurified in sucrose gradients with the 22S dynein, and a high molecular weight protein (greater than 300K) in the 19 S region were phosphorylated when cyclic AMP was added to permeabilized, motile cells. These data suggest that regulation of ciliary motility by cyclic AMP may include phosphorylation of dynein-associated proteins

Chronic neural probe for simultaneous recording of single-unit, multi-unit, and local field potential activity from multiple brain sites

Drug resistant focal epilepsy can be treated by resecting the epileptic focus requiring a precise focus localization using stereoelectroencephalography (SEEG) probes. As commercial SEEG probes offer only a limited spatial resolution, probes of higher channel count and design freedom enabling the incorporation of macro and microelectrodes would help increasing spatial resolution and thus open new perspectives for investigating mechanisms underlying focal epilepsy and its treatment. This work describes a new fabrication process for SEEG probes with materials and dimensions similar to clinical probes enabling recording single neuron activity at high spatial resolution. Polyimide is used as a biocompatible flexible substrate into which platinum electrodes and leads are... The resulting probe features match those of clinically approved devices. Tests in saline solution confirmed the probe stability and functionality. Probes were implanted into the brain of one monkey (Macaca mulatta), trained to perform different motor tasks. Suitable configurations including up to 128 electrode sites allow the recording of task-related neuronal signals. Probes with 32 and 64 electrode sites were implanted in the posterior parietal cortex. Local field potentials and multi-unit activity were recorded as early as one hour after implantation. Stable single-unit activity was achieved for up to 26 days after implantation of a 64-channel probe. All recorded signals showed modulation during task execution. With the novel probes it is possible to record stable biologically relevant data over a time span exceeding the usual time needed for epileptic focus localization in human patients. This is the first time that single units are recorded along cylindrical polyimide probes chronically implanted 22 mm deep into the brain of a monkey, which suggests the potential usefulness of this probe for human applications

Effective Average Action in N=1 Super-Yang-Mills Theory

For N=1 Super-Yang-Mills theory we generalize the effective average action Gamma_k in a manifest supersymmetric way using the superspace formalism. The exact evolution equation for Gamma_k is derived and, introducing as an application a simple truncation, the standard one-loop beta-function of N=1 SYM theory is obtained.Comment: 17 pages, LaTeX, some remarks added, misprints corrected, to appear in Phys. Rev.

Exact Flow Equations and the U(1)-Problem

The effective action of a SU(N)-gauge theory coupled to fermions is evaluated at a large infrared cut-off scale k within the path integral approach. The gauge field measure includes topologically non-trivial configurations (instantons). Due to the explicit infrared regularisation there are no gauge field zero modes. The Dirac operator of instanton configurations shows a zero mode even after the infrared regularisation, which leads to U_A(1)-violating terms in the effective action. These terms are calculated in the limit of large scales k.Comment: 22 pages, latex, no figures, with stylistic changes and some arguments streamlined, typos corrected, References added, to appear in Phys. Rev.

Multivalued Fields on the Complex Plane and Conformal Field Theories

In this paper a class of conformal field theories with nonabelian and discrete group of symmetry is investigated. These theories are realized in terms of free scalar fields starting from the simple $b-c$ systems and scalar fields on algebraic curves. The Knizhnik-Zamolodchikov equations for the conformal blocks can be explicitly solved. Besides of the fact that one obtains in this way an entire class of theories in which the operators obey a nonstandard statistics, these systems are interesting in exploring the connection between statistics and curved space-times, at least in the two dimensional case.Comment: (revised version), 30 pages + one figure (not included), (requires harvmac.tex), LMU-TPW 92-1

Exact and Truncated Dynamics in Nonequilibrium Field Theory

Nonperturbative dynamics of quantum fields out of equilibrium is often described by the time evolution of a hierarchy of correlation functions, using approximation methods such as Hartree, large N, and nPI-effective action techniques. These truncation schemes can be implemented equally well in a classical statistical system, where results can be tested by comparison with the complete nonlinear evolution obtained by numerical methods. For a 1+1 dimensional scalar field we find that the early-time behaviour is reproduced qualitatively by the Hartree dynamics. The inclusion of direct scattering improves this to the quantitative level. We show that the emergence of nonthermal temperature profiles at intermediate times can be understood in terms of the fixed points of the evolution equations in the Hartree approximation. The form of the profile depends explicitly on the initial ensemble. While the truncated evolution equations do not seem to be able to get away from the fixed point, the full nonlinear evolution shows thermalization with a (surprisingly) slow relaxation.Comment: 30 pages with 12 eps figures, minor changes; to appear in Phys.Rev.

The beta functions of a scalar theory coupled to gravity

We study a scalar field theory coupled to gravity on a flat background, below Planck's energy. Einstein's theory is treated as an effective field theory. Within the context of Wilson's renormalization group, we compute gravitational corrections to the beta functions and the anomalous dimension of the scalar field, taking into account threshold effects.Comment: 13 pages, plainTe

Winding number transitions at finite temperature in the Abelian-Higgs model

Following our earlier investigations we examine the quantum-classical winding number transition in the Abelian-Higgs system. It is demonstrated that the sphaleron transition in this system is of the smooth second order type in the full range of parameter space. Comparison of the action of classical vortices with that of the sphaleron supports our finding.Comment: final version, to appear in J. Phys.

Hard Non-commutative Loops Resummation

The non-commutative version of the euclidean $g^2\phi^4$ theory is considered. By using Wilsonian flow equations the ultraviolet renormalizability can be proved to all orders in perturbation theory. On the other hand, the infrared sector cannot be treated perturbatively and requires a resummation of the leading divergencies in the two-point function. This is analogous to what is done in the Hard Thermal Loops resummation of finite temperature field theory. Next-to-leading order corrections to the self-energy are computed, resulting in $O(g^3)$ contributions in the massless case, and $O(g^6\log g^2)$ in the massive one.Comment: 4 pages, 3 figures. The resummation procedure is now discussed also at finite ultraviolet cut-off. Minor changes in abstract and references. Final version to be published in Physical Review Letter