Isolasi dan Elusidasi Struktur Senyawa Lignan dan Asam Lemak dari Ekstrak Daging Buah Phaleria Macrocarpa

ISOLASI DAN ELUSIDASI STRUKTUR SENYAWA LIGNAN DAN ASAM LEMAK DARI EKSTRAK DAGING BUAH PHALERIA MACROCARP

Brine Shrimp Lethality Test (Bslt) Dari Berbagai Fraksi Ekstrak Daging Buah Dan Kulit Biji Mahkota Dewa (Phaleria Macrocarpa)

Biological activity of a natural product involved in several certain characteristics will influence its pharmaceutical application. Secondary metabolites, considered as chemical compounds, are now thought to mediate plant defense mechanism by providing chemical barriers against animal and microbial predators. Brine Shrimp Lethality Test (BSLT) method has been used as preliminary test for screening the activity of chemical compounds in n­ hexane, ethyl acetate, and methanol extracts from mesocarp and seeds of Phaleria macrocarpa, fam. Thymelaeaceae. BSLT method used shrimp larvas of Artemia salina L. to study the mortality effect that was caused by the sample extracts. All of crude extracts showed bioactivity with LC50 values from 0.16 to 11.83 µg/ml (baseline 1000 µg/ml). It means, at the concentration the crude extracts can cause 50% mortality of A. salina L. shrimp larvas, after 24 hours incubation. These results clearly indicate that crude extracts of P. macrocarpa showed high potential biological activity

Isolasi Dan Elusidasi Senyawa Kimia Dari Kulit Pohon Garcinia Tetrandra Pierre

A preliiminary study was carried out to isolate theconstituents of the stem bark of Garcinia tetran dra Pierre(Guttiferae). Several species of this family had been known asmedicinal herb, some other contain bioactive constituents andnovel compounds. The isolation were carried out by macerationin n-hexane where crude extract was purified usingchromatographic technique on SIG60 coloumn and n- hexane ethylacetate as eluents by gradient. Two pure compunds werefound and their molecular structures were determined on thebasis of combined spectroscopic methods UV, IR, 1H-NMR, l3C-NmR and MS and comparison with literature. The compouridhad been identified as xanton, 15-hydroxy-20, 21, 22, 23-tetramethyl-3H,11H-dipyrano (3, 2-a ; 2'. 3'-i) xanten-17-one(A), known as Thwaitesixanton and a triterpene as Hopenol - a(Hop-(22) (29)-en 3-0 ol) (B)

Isolation and Identification of Simiarenol From the Leaves of Ficus Aurantiacea Griff (Moraceae)

Ficus aurantiace a Griff (Mor aceae) collected from BukitKaba forest park, Bengkulu, was studied for the isolation andidentification of its bioactive constituents. This plant is a climbingtree, having large colored fruits, orange to red marbled withwhite spots. The methanol-soluble extract of the leaves was eluted10 silica-gel column chromatography several times using mixturesof chloroform-methanol as the elution solvent, leading to theisolation of a known compound, simiarenol. The structure ofsimiarenol was judged by its data comparison to published values,and based all the evaluation results of its physical andspectroscopic data. such as UV, IR, MS, one- and two-dimensional NMR, HMQC and HMBC

Solubility Enhancement of Ethyl AcetateFraction of the Artocarpus Altilis (Parkinson) Fosberg Leaves with Addition of β-Cyclodextrin-HPMC by Using Kneading Method

Ethyl acetate fraction of the Artocarpus altilis (Parkinson) Fosberg extract have a potency to treat the cardiovascular diseases have poorly solubility in water. The purpose of this study was to improve the solubility of the extract. One of method to improve the solubility of the extract by mixing with cyclodextrin polymers and their derivatives. Hydroxyl propyl methylcellulose (HPMC) as a water-soluble polymer can enhance the β-cyclodextrin (β-CD) activity. Three comparisons extract and ß-cyclodextrin were: 1:2, 1:4, and 1:6 by mixing with the addition hydroxyl propyl methylcellulose 0.12%of the total weight of extract and β-CD for each formula. The sample was prepared by kneading method. The sample characterization was used Karl Fischer titration, Scanning Electron Microscopy and solubility study.Content of total flavonoid from the extract was 32.7%. The Result showed that the addition polymer combination of β-CD + HPMC caused increasing the solubility of extract in water7.04% (F1), 19.47% (F2) and 59.92% (F3) compared to extract control with significant differences at level of confidence 95% (p ≤ 0.05)

Synthesis of Sulochrin-125I and Its Binding Affinity as α-Glucosidase Inhibitor Using Radioligand Binding Assay (RBA) Method

Most of diabetes patients have type 2 diabetes mellitus or non insulin dependent diabetes mellitus. Treatment of type 2 diabetes mellitus can be conducted by inhibiting the α-glucosidase enzyme which converts carbohydrates into glucose. Sulochrin is one of the potential compounds which can inhibit the function of α-glucosidase enzyme. This study was carried out to obtain data of sulochrin binding with α-glucosidase enzyme as α-glucosidase inhibitor using Radioligand Binding Assay (RBA) method. The primary reagent required in RBA is the labeled radioactive ligand (radioligand). In this study, the radioligand was sulochrin-125I. Prior to the sulochrin-125I synthesis, sulochrin-I was synthesized. Sulochrin-I and sulochrin-125I were synthesized and their bindings were studied using RBA. The molecular formula of the synthesized Sulochrin-I is C17H15O7I and its molecular weight is 457.9940. The sulochrin-125I was synthesized from sulochrin-I by isotope exchange method. From RBA, the dissociation constant (Kd) and the maximum binding (Bmax) were found as 26.316 nM and 9.302 nM, respectively. The low Kd indicates that sulochrin is capable of binding to α-glucosidase.Received: 08 November 2013; Revised: 28 February 2014; Accepted: 28 March 201