Internalization of the anti-carcinogenic IBB1, a major Bowman-Birk isoinhibitor from soybean (Glycine max), in HT29 colon cancer cells

Proceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín (Granada), en mayo de 2013.Protease inhibitors of the Bowman-Birk type, a major protease inhibitor family in legume seeds, which inhibit potently trypsin- and chymotrypsin-like proteases, are currently being investigated as colorectal chemopreventive agents. Although the therapeutic target/s and the action mechanism/s of Bowman-Birk inhibitors (BBI) have not yet been elucidated, the emerging evidence suggests that BBI exert their chemopreventive properties via protease inhibition; in this sense, serine proteases should be considered as primary targets in early stages of carcinogenesis. In this work, we have demonstrated that IBB1, a major protease inhibitor of the Bowman-Birk family in soybean (Glycine max), exerts anti-proliferative effect in human colorectal HT29 cancer cells at concentrations higher than 15 μM, in a dose dependent manner. By using confocal microscopy, we have demonstrated that IBB1 is taken up by HT29 colon cancer cells in a time-dependent manner, being the bulk of the internalized protease inhibitor localized in the cytoplasm where might interact with their potential therapeutic target/s.This work was supported by ERDF-co-financed grants AGL2011-26353 (Spanish Ministry of Economy and Competitiveness) and PE2010-CVI-5767 (Junta de Andalucía).Peer reviewe

Legumes as food ingredient: characterization, processing, and applications

Editores: Jiménez-López, José Carlos (CSIC); Clemente, Alfonso (CSIC

Characterization of Profilin Polymorphism in Pollen with a Focus on Multifunctionality

Profilin, a multigene family involved in actin dynamics, is a multiple partners-interacting protein, as regard of the presence of at least of three binding domains encompassing actin, phosphoinositide lipids, and poly-L-proline interacting patches. In addition, pollen profilins are important allergens in several species like Olea europaea L. (Ole e 2), Betula pendula (Bet v 2), Phleum pratense (Phl p 12), Zea mays (Zea m 12) and Corylus avellana (Cor a 2). In spite of the biological and clinical importance of these molecules, variability in pollen profilin sequences has been poorly pointed out up until now. In this work, a relatively high number of pollen profilin sequences have been cloned, with the aim of carrying out an extensive characterization of their polymorphism among 24 olive cultivars and the above mentioned plant species. Our results indicate a high level of variability in the sequences analyzed. Quantitative intra-specific/varietal polymorphism was higher in comparison to inter-specific/cultivars comparisons. Multi-optional posttranslational modifications, e.g. phosphorylation sites, physicochemical properties, and partners-interacting functional residues have been shown to be affected by profilin polymorphism. As a result of this variability, profilins yielded a clear taxonomic separation between the five plant species. Profilin family multifunctionality might be inferred by natural variation through profilin isovariants generated among olive germplasm, as a result of polymorphism. The high variability might result in both differential profilin properties and differences in the regulation of the interaction with natural partners, affecting the mechanisms underlying the transmission of signals throughout signaling pathways in response to different stress environments. Moreover, elucidating the effect of profilin polymorphism in adaptive responses like actin dynamics, and cellular behavior, represents an exciting research goal for the future

Assessment of the Allergenic Content of Olive Pollen from Portuguese Cultivars

1 página.-- Resumen de la comunicación presentada al 28º International Horticultural Congress celebrado del 22 al 27 de 2010 en Lisboa.-- S08.213 Olivetrends.Peer reviewe

Análisis preliminar del contenido alergénico del polen de variedades de olivo de Portugal

2 páginas.-- Comunicación presentada al XVI Simposio Internacional de Palinología de la A.P.L.E. celebrado en Mallorca (España) en Septiembre del 2008.Este trabajo ha sido financiado por los proyectos BFU 2004-00601/BFI (MEC), P06-AGR-01791 (Junta de Andalucía) y 2007PT0039 (CSIC/Fundaçao para a ciencia e a tecnología).Peer reviewe

Discrimination of Portuguese and Spanish olive cultivars using microsatellite markers

Resumen del trabajo presentado al 2nd International Symposium on Genomics of Plant Genetic Resources, P3.27, celebrado en Bolonia (Italia) del 24 al 27 de Abril de 2010.This work has been financed by the following projects: MEC BFU2008-00629, P06-AGR-01791 (J. Andalucía) and CSIC/Fundaçao Ciencia e Tecnología 2007PT0039.Peer reviewe

Lipid composition and associated gene expression patterns during pollen germination and pollen tube growth in Olea europaea L.

This work was supported by ERDF co-financed grants AGL2013-43042, AGL2017-84298 and BFU-2016-77243 from MINECO

Temperature and pyoverdine-mediated iron acquisition control surface motility of Pseudomonas putida

9 páginas, 6 figuras, 1 tabla.Pseudomonas putida KT2440 is unable to swarm at its common temperature of growth in the laboratory (30°C) but exhibits surface motility similar to swarming patterns in other Pseudomonas between 18°C and 28°C. These motile cells show differentiation, consisting on elongation and the presence of surface appendages. Analysis of a collection of mutants to define the molecular determinants of this type of surface movement in KT2440 shows that while type IV pili and lipopolysaccharide O-antigen are requisites flagella are not. Although surface motility of flagellar mutants was macroscopically undistinguishable from that of the wild type, microscopy analysis revealed that these mutants move using a distinct mechanism to that of the wild-type strain. Mutants either in the siderophore pyoverdine (ppsD) or in the FpvA siderophore receptor were also unable to spread on surfaces. Motility in the ppsD strain was totally restored with pyoverdine and partially with the wild-type ppsD allele. Phenotype of the fpvA strain was not complemented by this siderophore. We discuss that iron influences surface motility and that it can be an environmental cue for swarming-like movement in P. putida. This study constitutes the first report assigning an important role to pyoverdine iron acquisition in en masse bacterial surface movement.M.A. Matilla and M.I. Ramos-González are recipients of grants from the Spanish Ministry of Education and Science (MEC), and the Junta de Andalucía respectively. This work was supported by grants to group CV191 from the Junta de Andalucía and BFU2006-09078/BMC to M.I.R.G. from MEC.Peer reviewe