27 research outputs found
Role of viral infection in the etiopathogenesis of breast cancer
The viral nature of many female genital cancers is now beyond question; however, the role of viral infection in the pathogenesis of breast cancer (BC) has not been adequately investigated. The paper defines the importance of a number of viruses in the etiopathogenesis of on- cogynecological diseases. It presents the results of examining 60 patients with Stages I-IV BC and 30 patients with fibrocystic mastopathy, in whom the presence of DNA-containing virus genomes in tumor tissue was compared, and the data of polymerase chain reaction study of genital tract smears. It is shown that human papillomaviruses and cytomegaloviruses do not play a fundamental role in the develop- ment of BC; there is no valid evidence for EpsteinβBarr virus
The recurrences of cervical cancer: Possibilities of molecular prediction
The incidence of recurrence of cervical cancer ranges from 10 to 40 %. The 5-year survival rate for patients with recurrent cervical cancer is about 5β15 % against the background of current drug therapy. Clinical and morphological characteristics of the tumor process are known, which are used as markers of an unfavorable prognosis for the development of cervical cancer recurrence. The search for molecular prognostic markers of the course of cervical cancer continues.The aim. To determine the level of immune cycle proteins in patients with cervical cancer 0βIV stages, depending on the occurrence of a relapse of the disease.Materials and research methods. A retrospective analysis of previously obtained results of a study on the local level of immune cycle proteins in patients with cervical cancer was performed. Three years after follow-up, 2 groups were formed: group 1 β patients treated for cervical cancer without signs of disease progression (n = 83); group 2 β patients with cervical cancer with local or systemic recurrence (n = 18). Used statistical methods: non-parametric methods of statistics using the Kruskal β Wallis test; ROC-analysis for significant values in order to calculate threshold values; determination of the quality of the identified predictive markers by calculating the sensitivity, specificity, accuracy.Results. Local initial threshold values have a predictive value for predicting the occurrence of cervical cancer recurrence: B7.2 < 10.7 pg/ml (Se = 0.87; Sp = 0.73; Ac = 0.76; AUC = 0.78), PD-L1 β€ 5.1 pg/ml (Se = 0.87; Sp = 0.68; Ac = 0.71; AUC = 0.76), sCD27 β₯ 32.0 pg/ml (Se = 0.75; Sp = 0.78; Ac = 0.78; AUC = 0.75).Conclusion. Determination of local levels of B7.2, PD-L1, sCD27 in patients with cervical cancer before treatment can be used to predict the development of disease recurrence during 3 years of follow-up
Complex ultrasound diagnostic assessment of the results of neoadjuvant chemotherapy for locally advanced cervical cancer (Stages IIBβIIIB)
Background. Current complex ultrasound diagnosis using novel imaging techniques can assess, to a high accuracy, different tumor parametersΒ during neoadjuvant chemotherapy (NCT) for locally advanced cervical cancer (CC) (Stages IIBβIIB). This assessment is very important andΒ necessary to define further treatment policy.Materials and methods. A total of 199 patients diagnosed with Stages IIBβIIIB CC, including 60 patients with Stage IIB (T2bN0M0), 4 withΒ Stage IIIΠ (T3aN0M0), and 135 with Stage IIIΠ (T2bN1M0, T3aN1M0, T3bN0β1M0) (according to the International Federationof Gynecology and Obstetrics (FIGO) classification), who received NCT at Stage 1 of treatment, were examined. Complex ultrasound studyΒ was conducted before treatment initiation and after each NCT cycle. The therapeutic pathomorphism of a tumor was evaluated in surgicallyΒ treated patients.Results. The criteria have been determined for evaluating the efficiency of NCT for locally advanced CC, which are based on current ultrasonographicΒ techniques including B-mode, Doppler ultrasound (power, spectral, three-dimensional ones), as well as on the results of therapeuticΒ pathomorphism.Conclusion. The criteria for evaluating the efficiency of NCT for CC should be based on current complex ultrasonographic techniques
ΠΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½-Ξ³ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΠΉ ΡΠΎΡΡ
Purpose of the study: to analyze published data on the mechanisms of action of interferon gamma (IFN-Ξ³) in tumor growth and to evaluate the possibility of its use in the treatment of solid tumors. Material and Methods. More than 200 publications were found in the Scopus, Pubmed, eLibrary and other databases, the search keywords were: interferon gamma, tumor growth, cancer therapy. This review includes 54 papers. Results. IFN-Ξ³ is a pleiotropic cytokine with antiviral, antitumor, and immunomodulatory functions and plays an important role in coordinating the innate and adaptive immune response. The success of immuno-oncology drugs and chemotherapy in the treatment of malignant tumors depends on the stimulation of the production and adequate signaling of IFN-Ξ³. Suppression and loss of IFN-Ξ³ receptor and downstream signaling mediators, and amplifcation of molecules that inhibit the IFN-Ξ³ signaling pathway are common mechanisms for tumor cells to escape from the immune system. The development of malignant processes is accompanied by a change, more often a decrease, in the secretion of IFN-Ξ³, which attracts the attention of researchers to its exogenous administration. Determination of the IFN-Ξ³ signature may be a predictive marker of clinical response to anticancer drug therapy. The antitumor properties of IFN-Ξ³ are largely dose-dependent, which has been clearly shown in clinical and experimental studies. Low doses of the drug often promote tumor growth. On the contrary, the use of high doses is usually accompanied by an antitumor effect. IFN-Ξ³ or its inducers remain promising agents for cancer therapy. Combinatorial strategies involving IFN-Ξ³ may be a rational option to overcome tumor resistance to blockade of immune checkpoints. Conclusion. It is necessary to continue fundamental and applied research to study the feasibility of using interferon gamma as a therapeutic agent in tumor growth.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΏΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΠΎΠΏΡΠ±Π»ΠΈΠΊΠΎΠ²Π°Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΎ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠ°Ρ
Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π³Π°ΠΌΠΌΠ° (IFN-Ξ³) ΠΏΡΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΌ ΡΠΎΡΡΠ΅ ΠΈ ΠΎΡΠ΅Π½ΠΈΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ Π΅Π³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠΎΠ»ΠΈΠ΄Π½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΎ ΡΠ΅ΠΌΠ΅ Π½Π°ΠΉΠ΄Π΅Π½ΠΎ Π±ΠΎΠ»Π΅Π΅ 200 Π·Π°ΡΡΠ±Π΅ΠΆΠ½ΡΡ
ΠΈ ΡΠΎΡΡΠΈΠΉΡΠΊΠΈΡ
ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΉ, ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΡΡ
Π² Π±Π°Π·Π°Ρ
Π΄Π°Π½Π½ΡΡ
Scopus, Pubmed, eLibrary ΠΈ Π΄ΡΡΠ³ΠΈΡ
; ΠΊΠ»ΡΡΠ΅Π²ΡΠ΅ ΡΠ»ΠΎΠ²Π° ΠΏΠΎΠΈΡΠΊΠ°: ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½ Π³Π°ΠΌΠΌΠ°, ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠΉ ΡΠΎΡΡ, ΡΠ΅ΡΠ°ΠΏΠΈΡ ΡΠ°ΠΊΠ°. Π Π΄Π°Π½Π½ΡΠΉ ΠΎΠ±Π·ΠΎΡ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΎ 54 ΡΠ°Π±ΠΎΡΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. IFN-Ξ³ β ΠΏΠ»Π΅ΠΉΠΎΡΡΠΎΠΏΠ½ΡΠΉ ΡΠΈΡΠΎΠΊΠΈΠ½, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠΉ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΈΡΡΡΠ½ΠΎΠΉ, ΠΏΡΠΎΡΠΈΠ²ΠΎΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠΌΠΎΠ΄ΡΠ»ΠΈΡΡΡΡΠ΅ΠΉ ΡΡΠ½ΠΊΡΠΈΡΠΌΠΈ ΠΈ ΠΈΠ³ΡΠ°ΡΡΠΈΠΉ Π²Π°ΠΆΠ½ΡΡ ΡΠΎΠ»Ρ Π² ΠΊΠΎΠΎΡΠ΄ΠΈΠ½Π°ΡΠΈΠΈ Π²ΡΠΎΠΆΠ΄Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈ Π°Π΄Π°ΠΏΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ°. Π£ΡΠΏΠ΅ΡΠ½ΠΎΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ ΠΈΠΌΠΌΡΠ½ΠΎΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΈ Ρ
ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π·Π°Π²ΠΈΡΠΈΡ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅, ΠΎΡ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π²ΡΡΠ°Π±ΠΎΡΠΊΠΈ ΠΈ Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½ΠΎΠΉ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ ΡΠΈΠ³Π½Π°Π»ΠΎΠ² IFN-Ξ³. ΠΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠ΅ ΠΈ ΠΏΠΎΡΠ΅ΡΡ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠ° IFN-Ξ³ ΠΈ Π½ΠΈΠΆΠ΅ΡΡΠΎΡΡΠΈΡ
ΡΠΈΠ³Π½Π°Π»ΡΠ½ΡΡ
ΠΌΠ΅Π΄ΠΈΠ°ΡΠΎΡΠΎΠ², Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ», ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠΈΡ
ΡΠΈΠ³Π½Π°Π»ΡΠ½ΡΠΉ ΠΏΡΡΡ IFN-Ξ³, ΡΠ²Π»ΡΡΡΡΡ ΠΎΠ±ΡΡΠ½ΡΠΌΠΈ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠ°ΠΌΠΈ ΡΡΠΊΠΎΠ»ΡΠ·Π°Π½ΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΎΡ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ. Π Π°Π·Π²ΠΈΡΠΈΠ΅ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π΅ΡΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ (ΡΠ°ΡΠ΅ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ΠΌ), ΡΠ΅ΠΊΡΠ΅ΡΠΈΠΈ IFN-Ξ³, ΡΡΠΎ ΠΏΡΠΈΠ²Π»Π΅ΠΊΠ°Π΅Ρ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»Π΅ΠΉ ΠΊ Π΅Π³ΠΎ ΡΠΊΠ·ΠΎΠ³Π΅Π½Π½ΠΎΠΌΡ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΈΠ³Π½Π°ΡΡΡΡ IFN-Ξ³ ΠΌΠΎΠΆΠ΅Ρ ΡΠ²Π»ΡΡΡΡΡ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° Π½Π° ΠΏΡΠΎΡΠΈΠ²ΠΎΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ ΡΠ΅ΡΠ°ΠΏΠΈΡ. ΠΡΠΎΡΠΈΠ²ΠΎΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° IFN-Ξ³ Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΎΠΌ Π΄ΠΎΠ·ΠΎΠ·Π°Π²ΠΈΡΠΈΠΌΡ, ΡΡΠΎ Π½Π°Π³Π»ΡΠ΄Π½ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΡ
. ΠΠΈΠ·ΠΊΠΈΠ΅ Π΄ΠΎΠ·Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΡΠ°ΡΠ΅ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΡΡ ΡΠΎΡΡΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ, Π½Π°ΠΏΡΠΎΡΠΈΠ², ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΡΡΠΎΠΊΠΈΡ
Π΄ΠΎΠ· ΠΎΠ±ΡΡΠ½ΠΎ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π΅ΡΡΡ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠΌ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ. IFN-Ξ³ ΠΈΠ»ΠΈ Π΅Π³ΠΎ ΠΈΠ½Π΄ΡΠΊΡΠΎΡΡ ΠΎΡΡΠ°ΡΡΡΡ ΠΌΠ½ΠΎΠ³ΠΎΠΎΠ±Π΅ΡΠ°ΡΡΠΈΠΌΠΈ Π°Π³Π΅Π½ΡΠ°ΠΌΠΈ Π΄Π»Ρ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΉ. ΠΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΎΡΠ½ΡΠ΅ ΡΡΡΠ°ΡΠ΅Π³ΠΈΠΈ Ρ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ IFN-Ξ³ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΡΠ°ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΌ Π²Π°ΡΠΈΠ°Π½ΡΠΎΠΌ Π΄Π»Ρ ΠΏΡΠ΅ΠΎΠ΄ΠΎΠ»Π΅Π½ΠΈΡ ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ ΠΊ Π±Π»ΠΎΠΊΠ°Π΄Π΅ ΠΈΠΌΠΌΡΠ½Π½ΡΡ
ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΡ
ΡΠΎΡΠ΅ΠΊ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠ΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠ°ΡΡ ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΠ΅ ΠΈ ΠΏΡΠΈΠΊΠ»Π°Π΄Π½ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠ΅ΠΉ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° Π³Π°ΠΌΠΌΠ° Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π»Π΅ΡΠ΅Π±Π½ΠΎΠ³ΠΎ Π°Π³Π΅Π½ΡΠ° ΠΏΡΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΌ ΡΠΎΡΡΠ΅
Proteome-metabolome profiling of ovarian cancer ascites reveals novel components involved in intercellular communication
Β© 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Ovarian cancer ascites is a native medium for cancer cells that allows investigation of their secretome in a natural environment. This medium is of interest as a promising source of potential biomarkers, and also as a medium for cell-cell communication. The aim of this study was to elucidate specific features of the malignant ascites metabolome and proteome. In order to omit components of the systemic response to ascites formation, we compared malignant ascites with cirrhosis ascites. Metabolome analysis revealed 41 components that differed significantly between malignant and cirrhosis ascites. Most of the identified cancer-specific metabolites are known to be important signaling molecules. Proteomic analysis identified 2096 and 1855 proteins in the ovarian cancer and cirrhosis ascites, respectively; 424 proteins were specific for the malignant ascites. Functional analysis of the proteome demonstrated that the major differences between cirrhosis and malignant ascites were observed for the cluster of spliceosomal proteins. Additionally, we demonstrate that several splicing RNAs were exclusively detected in malignant ascites, where they probably existed within protein complexes. This result was confirmed in vitro using an ovarian cancer cell line. Identification of spliceosomal proteins and RNAs in an extracellular medium is of particular interest; the finding suggests that they might play a role in the communication between cancer cells. In addition, malignant ascites contains a high number of exosomes that are known to play an important role in signal transduction. Thus our study reveals the specific features of malignant ascites that are associated with its function as a medium of intercellular communication
Possible pathogenetic types of sporadical ovarian cancer
This article in question dwells on a possible pathogenetic model ovarian cancer, itβs histogenesis speciality, the role of ovulation, chronic in- flammation and stem cells. The scheme of two variant of avarian cancer progress and possible ways of prevention it are represented as well
Molecular biological factors in the diagnosis of cervical intraepithelial neoplasias
The authors have made a complex analysis of the molecular biological factors associated with cervical intraepithelial neoplasia. They have revealed that infection by oncogenic human papillomavirus types is associated with suppressed apoptosis and enhanced cellular proliferative activity, which can be effectively used in the diagnosis and prediction of cervical neoplasias to optimize management tac- tics and to improve the results of treatment
Intra-nosological diagnosis of endometrial cancer
The study is based on the analysis of comprehensive ultrasound examinations of 158 patients with a verified diagnosis of endometrial cancer (EC). It was conducted, by taking into account the concept of 3 types of microinvasive EC growth. Echosemiotics in each of these types is detailed. Ultrasound criteria for diagnosing the microinvasive process of EC, tumor invasion into the cervical canal are identified