Nitrogen-doped carbon nanotubes: growth, mechanism and structure

Nitrogen‐doped bamboo‐structured carbon nanotubes have been successfully grown using a series of cobalt/molybdenum catalysts. The morphology and structure of the nanotubes were analysed by transmission electron microscopy and Raman spectroscopy. The level of nitrogen doping, as determined by X‐ray photoelectron spectroscopy, was found to range between 0.5 to 2.5 at. %. The growth of bamboo‐structured nanotubes in the presence of nitrogen, in preference to single‐walled and multi‐walled nanotubes, was due to the greater binding energy of nitrogen for cobalt in the catalyst compared to the binding strength of carbon to cobalt, as determined by density functional theory

Identification of Sare0718 As an Alanine-Activating Adenylation Domain in Marine Actinomycete Salinispora arenicola CNS-205

BACKGROUND: Amino acid adenylation domains (A domains) are critical enzymes that dictate the identity of the amino acid building blocks to be incorporated during nonribosomal peptide (NRP) biosynthesis. NRPs represent a large group of valuable natural products that are widely applied in medicine, agriculture, and biochemical research. Salinispora arenicola CNS-205 is a representative strain of the first discovered obligate marine actinomycete genus, whose genome harbors a large number of cryptic secondary metabolite gene clusters. METHODOLOGY/PRINCIPAL FINDINGS: In order to investigate cryptic NRP-related metabolites in S. arenicola CNS-205, we cloned and identified the putative gene sare0718 annotated "amino acid adenylation domain". Firstly, the general features and possible functions of sare0718 were predicted by bioinformatics analysis, which suggested that Sare0718 is a soluble protein with an AMP-binding domain contained in the sequence and its cognate substrate is L-Val. Then, a GST-tagged fusion protein was expressed and purified to further explore the exact adenylation activity of Sare0718 in vitro. By a newly mentioned nonradioactive malachite green colorimetric assay, we found that L-Ala but not L-Val is the actual activated amino acid substrate and the basic kinetic parameters of Sare0718 for it are K(m) = 0.1164±0.0159 (mM), V(max) = 3.1484±0.1278 (µM/min), k(cat) = 12.5936±0.5112 (min(-1)). CONCLUSIONS/SIGNIFICANCE: By revealing the biochemical role of sare0718 gene, we identified an alanine-activating adenylation domain in marine actinomycete Salinispora arenicola CNS-205, which would provide useful information for next isolation and function elucidation of the whole cryptic nonribosomal peptide synthetase (NRPS)-related gene cluster covering Sare0718. And meanwhile, this work also enriched the biochemical data of A domain substrate specificity in newly discovered marine actinomycete NRPS system, which bioinformatics prediction will largely depend on

Nitrogen-doped carbon nanotubes: growth, mechanism and structure

Nitrogen‐doped bamboo‐structured carbon nanotubes have been successfully grown using a series of cobalt/molybdenum catalysts. The morphology and structure of the nanotubes were analysed by transmission electron microscopy and Raman spectroscopy. The level of nitrogen doping, as determined by X‐ray photoelectron spectroscopy, was found to range between 0.5 to 2.5 at. %. The growth of bamboo‐structured nanotubes in the presence of nitrogen, in preference to single‐walled and multi‐walled nanotubes, was due to the greater binding energy of nitrogen for cobalt in the catalyst compared to the binding strength of carbon to cobalt, as determined by density functional theory

Species co-occurrences in ectoparasite infracommunities: Accounting for confounding factors associated with space, time, and host community composition

1. We explored patterns of co-occurrence of ectoparasite species on individual hosts from Central Europe (Slovakia) and South America (Argentina) within and between higher taxa while controlling for confounding factors associated with variation between host individuals and host species, as well as spatial and temporal variation. We used a recently developed statistical approach, the hierarchical modelling of species communities. 2. Although the probability of pairwise associations of the majority of species in both regions did not differ from random, all significant species associations in Slovakia were positive, whereas the only three significant species associations in Argentina were negative. On average, associations between ectoparasite species belonging to different higher taxa were positive in Slovakia and negative in Argentina. 3. At the host species level, both positive and negative associations between species were detected in all higher taxa. This was also true for ectoparasite co-occurrences in the same site, habitat, or year; although the number of co-occurring species pairs with high posterior probability was much greater in Slovakia than in Argentina. 4. We conclude that consideration of species co-occurrences across the entire multi-host assemblage and control for confounding spatial and temporal factors provided important insights into parasite community structure.Fil: Krasnov, Boris R.. Ben Gurion University of the Negev; IsraelFil: Stanko, Michal. Slovak Academy of Sciences. Institute of Parasitology and Institute of Zoology; EslovaquiaFil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Khokhlova, Irina S.. Ben Gurion University of the Negev; Israe