The earlier identification of the seedless characteristic of the wampee [Clausena lansium (Lour.) Skeels] hybrid by a random amplified polymorphic DNA (RAPD) marker

Wampee as an important fruit is widely grown in South China and conventional breeding methods for the production of new seedless wampee cultivars in recent years were operated. Hybridization wasconducted using the seed as female parents and the seedless as male parents. However, the seeds could be gotten from cross combination. An efficient operation was that, non-woven fabrics bag could increase burliness rate in hybridization. In order to earlier identify seedless seedlings in hybrid progeny, the objective of this work is to identify molecular markers linked to seedless trait through bulked segregant analysis. Two bulks, seedless and seed, were prepared by pooling DNA of individual identified plants and used in polymerase chain reaction (PCR) analysis. Out of the 100 primers tested, only S06 primer could get a specific 930 bp restriction random amplified polymorphic DNA (RAPDamplicon to seedless bulk. As a result, a hybrid was tested with this primer in two cross combinations.Keywords: Earlier identification, seedless wampee, bulked segregant analysis, randomly amplified polymorphic DN

Therapeutic plasma exchange for neuromyelitis optica spectrum disorder: A multicenter retrospective study by the ASFA neurologic diseases subcommittee

© 2019 Wiley Periodicals, Inc. Importance: Neuromyelitis optica/neuromyelitis optica spectrum disorder patients\u27 response to therapeutic plasma exchange (TPE) is currently incompletely characterized. Objective: Our study aims to understand the clinical status improvement of neuromyelitis optica/neuromyelitis optica spectrum disorder patients treated with TPE. Design, Setting, and Participants: This is a multicenter retrospective study conducted between 1 January 2003 and 31 July 2017 at 13 US hospitals performing apheresis procedures. Subjects studied were diagnosed with neuromyelitis optica/neuromyelitis optica spectrum disorder who received TPE during presentation with acute disease. Main Outcomes and Measures: The primary outcome was clinical status improvement in patients treated with TPE. Secondary measures were procedural and patient characteristics associated with response to treatment. Results: We evaluated 114 patients from 13 institutions. There was a female predilection. The largest ethnic group affected was non-Hispanic Caucasian. The average age of diagnosis was 43.1 years. The average time to diagnosis was 3.1 years. On average, five procedures were performed during each treatment series. The most commonly performed plasma volume exchange was 1.0 to 1.25 using 5% albumin as replacement fluid. Most patients (52%) did not require an additional course of TPE and noted “mild” to “moderate” clinical status improvement. Maximal symptom improvement appeared by the fourth or fifth TPE treatment. Conclusion and Relevance: TPE improved the clinical status of patients. Adults responded more favorably than children. Procedural characteristics, including number of TPEs, plasma volume exchanged, and replacement fluid used, were similar between institutions. TPE was well-tolerated and had a low severe adverse event profile

Therapeutic plasma exchange for neuromyelitis optica spectrum disorder: A multicenter retrospective study by the ASFA neurologic diseases subcommittee.

© 2019 Wiley Periodicals, Inc. Importance: Neuromyelitis optica/neuromyelitis optica spectrum disorder patients\u27 response to therapeutic plasma exchange (TPE) is currently incompletely characterized. Objective: Our study aims to understand the clinical status improvement of neuromyelitis optica/neuromyelitis optica spectrum disorder patients treated with TPE. Design, Setting, and Participants: This is a multicenter retrospective study conducted between 1 January 2003 and 31 July 2017 at 13 US hospitals performing apheresis procedures. Subjects studied were diagnosed with neuromyelitis optica/neuromyelitis optica spectrum disorder who received TPE during presentation with acute disease. Main Outcomes and Measures: The primary outcome was clinical status improvement in patients treated with TPE. Secondary measures were procedural and patient characteristics associated with response to treatment. Results: We evaluated 114 patients from 13 institutions. There was a female predilection. The largest ethnic group affected was non-Hispanic Caucasian. The average age of diagnosis was 43.1 years. The average time to diagnosis was 3.1 years. On average, five procedures were performed during each treatment series. The most commonly performed plasma volume exchange was 1.0 to 1.25 using 5% albumin as replacement fluid. Most patients (52%) did not require an additional course of TPE and noted “mild” to “moderate” clinical status improvement. Maximal symptom improvement appeared by the fourth or fifth TPE treatment. Conclusion and Relevance: TPE improved the clinical status of patients. Adults responded more favorably than children. Procedural characteristics, including number of TPEs, plasma volume exchanged, and replacement fluid used, were similar between institutions. TPE was well-tolerated and had a low severe adverse event profile